Cloning and characterization of novel low molecular weight glutenin subunit genes from two Aegilops species with the C and D genomes

Chen, Peng; Li, Ru; Zhou, Ran; Zhiguo, E; He, Guoqing

doi:10.1007/s10722-009-9527-y

Cited by 4 publications

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References 34 publications

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“…Wheat dough possesses unique viscoelastic properties determined by the structure and interaction of storage proteins in making bread and noodles [ 1 ]. Wheat storage proteins are mainly composed of monomeric gliadins and polymeric glutenins [ 2 , 3 ], and these glutenins are divided into the low-molecular-weight glutenin subunits (LMW-GS) and the high-molecular-weight glutenin subunits (HMW-GS) according to their electrophoretic mobility in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) [ 4 ]. The HMW-GS have been recognized as the major determinants of dough and gluten properties [ 5 ], but LMW-GS also play an important role in determining wheat dough's viscoelastic properties [ 6 ].…”

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confidence: 99%

Cloning and Characterization of Low-Molecular-Weight Glutenin Subunit Alleles from Chinese Wheat Landraces (Triticum aestivumL.)

Zhao

Zhang

et al. 2014

The Scientific World Journal

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Low-molecular-weight glutenin subunits (LMW-GS) are of great importance in processing quality and participate in the formation of polymers in wheat. In this study, eight new LMW-GS alleles were isolated from Chinese wheat landraces (Triticum aestivum L.) and designated as Glu-A3-1a, Glu-A3-1b, Glu-B3-1a, Glu-B3-1b, Glu-B3-1c, Glu-D3-1a, Glu-D3-1b, and Glu-D3-1c, which were located at the Glu-A3, Glu-B3, and Glu-D3 loci, respectively. Based on the proteins encoded, the number of deduced amino acids of Glu-B3 alleles was approximately 50 more than those of Glu-A3 and Glu-D3 alleles. The first cysteine of Glu-A3 and Glu-D3 alleles was located at the N-terminal domain, while that of Glu-B3 alleles was found in the repetitive domain, which may lead to the different functioning in forming disulfide bonds. All the eight genes were LMW-m types and the new allele of Glu-B3-1a which had nine cysteine residues may be the desirable LMW-GS gene for improving bread-making quality.

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Section: Introductionmentioning

confidence: 99%

Cloning and Characterization of Low-Molecular-Weight Glutenin Subunit Alleles from Chinese Wheat Landraces (Triticum aestivumL.)

Zhao

Zhang

et al. 2014

The Scientific World Journal

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Molecular characterisation of novel LMW-m and LMW-s genes from four Aegilops species (Sitopsis section) and comparison with those from the Glu-B3 locus of common wheat

2016

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Low-molecular-weight glutenin subunits (LMW-GS) are a component of the gluten network and play a key role in determining the viscoelastic properties of wheat dough. Aegilops species have been shown to be an important source of variation for valuable traits for wheat breeding. However, very little is known about LMW-GS genes in section Sitopsis species, which are closely related to the B genome of common wheat. Ten accessions of Sitopsis species were evaluated for variability of LMW-GS genes, and 20 novel genes were obtained, of which nine were LMW-m and 11 were LMW-s genes. Only two were pseudogenes, corresponding to one LMW-m and one LMW-s gene. Six groups of genes were detected: three for each of the LMW-m and LMW-s genes. All groups of LMW-s genes and one of LMW-m genes (pGluU) detected were not related to B-genome genes from common wheat, whereas the remaining genes were. The single-nucleotide polymorphisms, and insertions and deletions, detected in active variants compared with those from common wheat could affect structure protein. The analysis of reactive epitopes for coeliac disease revealed that LMW-s subunits lacked toxicity, as did the pGluU LMW-m subunits; the other LMW-m subunits were less toxic than that from common wheat.

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Molecular characterization of LMW-GS genes from C, N, U and S<sup>s</sup> genomes among <i>Aegilops</i> species

Wang¹,

Chen²,

Guo³

et al. 2012

Cereal Research Communications

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Cloning and characterization of novel low molecular weight glutenin subunit genes from two Aegilops species with the C and D genomes

Cited by 4 publications

References 34 publications

Cloning and Characterization of Low-Molecular-Weight Glutenin Subunit Alleles from Chinese Wheat Landraces (Triticum aestivumL.)

Cloning and Characterization of Low-Molecular-Weight Glutenin Subunit Alleles from Chinese Wheat Landraces (Triticum aestivumL.)

Molecular characterisation of novel LMW-m and LMW-s genes from four Aegilops species (Sitopsis section) and comparison with those from the Glu-B3 locus of common wheat

Molecular characterization of LMW-GS genes from C, N, U and S<sup>s</sup> genomes among <i>Aegilops</i> species

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