Cloning and characterization of novel microsatellite DNA markers for the grass rockfish, <i>Sebastes rastrelliger</i>, and cross‐species amplification in 10 related <i>Sebastes</i> spp

Westerman, Mark; Buonaccorsi, Vincent P.; Stannard, Jason A.; Galver, Luana; Taylor, Cynthia; Lynn, Eric A.; Kimbrell, Carol A.; Vetter, Russell D.

doi:10.1111/j.1471-8286.2004.00837.x

Cited by 39 publications

(15 citation statements)

References 6 publications

(5 reference statements)

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“…5 alleles) loci on samples of > 20 larvae, it was expected that our use of 100 larvae and 5 loci was sufficient to address this issue. All loci were amplified by polymerase chain reaction (PCR) following the conditions described in Gomez-Uchida et al (2003) and Westerman et al (2005). Fluorescently labeled PCR products were separated by size via electrophoresis through either a 6% polyacrylamide gel (using an ABI 377XL automated sequencer) or capillaries filled with POP-7 polyacrylamide polymer (using an ABI 3130XL Genetic Analyzer).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Multiple paternity and maintenance of genetic diversity in the live-bearing rockfishes Sebastes spp.

Hyde

Kimbrell²,

Robertson³

et al. 2008

Mar. Ecol. Prog. Ser.

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Section: Methodsmentioning

confidence: 99%

“…Microsatellite loci were chosen from the libraries developed by Westerman et al (2005) (Sra5-9.1, Sra7-2.2, ) and GomezUchida et al (2003) (Spi4, Spi6, Spi10). All fish within the captive populations were genotyped at 6 (Sebastes rastrelliger) or 8 (S. ruberrimus) microsatellite loci (see Table 1).…”

Section: Methodsmentioning

confidence: 99%

Multiple paternity and maintenance of genetic diversity in the live-bearing rockfishes Sebastes spp.

Hyde

Kimbrell²,

Robertson³

et al. 2008

Mar. Ecol. Prog. Ser.

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“…For all adults, 22 previously published microsatellite markers (Table 1; Roques et al 1999, Wimberger et al 1999, Miller et al 2000, Gomez-Uchida et al 2003, Westerman et al 2005 were genotyped and tested for utility in parentage analysis. Locus-bylocus deviations from Hardy-Weinberg equilibrium and inbreeding coefficients were as sessed using Genetix v4.05.2 (Table 1; Belkhir et al 2004).…”

Section: Parentage Analysismentioning

confidence: 99%

Mate selection in captive-breeding rockfishes Sebastes spp.: inference from parentage analysis and the major histocompatibility complex (MHC)

Johansson¹,

Clifford²,

Fodness³

et al. 2012

Mar. Ecol. Prog. Ser.

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Rockfish species of the genus Sebastes are notable for being numerous and diverse. Rockfishes are unusual among fish because they fertilize their eggs internally and release live, swimming larvae. They undergo complex courting behaviors, which may allow females to be selective about their mates. The major histocompatibility complex (MHC) is implicated as having an important influence on mate selection in other fishes, especially in sticklebacks and salmonids. Research suggests that females choose mates that optimize the MHC genotypes of their offspring. Previous research on rockfishes indicates that multiple functional MHC sequences may be found in each species, and that multiple mating is common in the genus, possibly as a bet-hedging strategy against uncertain or incomplete mate-selection information. In this project, we characterized the MHC genotypes of copper (S. caurinus) and quillback (S. maliger) rockfish parents, assessed parentage of 14 larval broods, and assessed the MHC genotypes of the parents to determine if MHC-mediated mate choice was occurring. As in previous studies, we found that rockfishes possess multiple, highly variable MHC genes, and that females may mate with multiple males. We also found evidence of female preference for particular males. However, we found no strong evidence of selection based on MHC genotype. Females were not consistently selective based on relatedness, allele count, proportion of shared alleles, or minimum, mean, or maximum DNA or amino acid genetic distance. Instead, it appears that females were selective based on other measures of mate quality not considered in this study, with some hedging of bets through multiple mating also occurring.

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“…From the extracted genomic DNA, we amplified 6 micro satellite loci de signed from Sebastes rastrelliger , GenBank Accession numbers: AF269054-57, AF269059, AF 269061, respectively; Buonaccorsi et al 2004, Westerman et al 2005, using polymerase chain reaction (PCR). We followed the protocol outlined in Burford & Lar son (2007) and Burford & Bernardi (2008) for fragment amplification and scoring of these 6 microsatellite loci.…”

Section: Dna Extraction and Amplificationmentioning

confidence: 99%

Age-structured genetic analysis reveals temporal and geographic variation within and between two cryptic rockfish species

Burford

Carr²,

Bernardi³

2011

Mar. Ecol. Prog. Ser.

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The spatial patterns of genetic structure among juveniles of long-lived species can reveal the extent of interannual and geographic variation in realized larval dispersal as well as the processes that determine ecologically relevant patterns of population connectivity. However, few studies examine this temporal and spatial variation over large portions of a species' geographic range or between cryptic species that overlap in their range. Despite the potential for long-distance dispersal in blue rockfish Sebastes mystinus, a previous study of adults revealed two geographically distinct, cryptic species. To determine year-to-year variation in the patterns of spatial connectivity and to elucidate the potential ecological mechanisms involved in shaping new yearclasses and maintaining cryptic species, we sampled juveniles within California, USA, where both cryptic species coexist. Using microsatellite markers, we found geographic and temporal variation in the number of individuals from these distinct cryptic species within 2 new year-classes of juvenile S. mystinus. We also found differences in the geographic patterns of genetic structure of the 2 cryptic species. Whereas one species exhibited little or no spatial genetic structure across the study region, the other exhibited a complex geographic pattern of genetic structure, with little or no genetic structure among regions, but small-scale structure within a region. The results of this study demonstrate that the spatial scales and patterns of realized dispersal of pelagic larvae vary geographically, interannually, and between closely related species with similar life-history strategies. Therefore, estimates of dispersal based on larval duration and patterns of adult structure need to be interpreted cautiously.

show abstract

Cloning and characterization of novel microsatellite DNA markers for the grass rockfish, Sebastes rastrelliger, and cross‐species amplification in 10 related Sebastes spp

Cited by 39 publications

References 6 publications

Multiple paternity and maintenance of genetic diversity in the live-bearing rockfishes Sebastes spp.

Multiple paternity and maintenance of genetic diversity in the live-bearing rockfishes Sebastes spp.

Mate selection in captive-breeding rockfishes Sebastes spp.: inference from parentage analysis and the major histocompatibility complex (MHC)

Age-structured genetic analysis reveals temporal and geographic variation within and between two cryptic rockfish species

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