Cloning and characterization of myr 6, an unconventional myosin of the dilute/myosin-V family.

Zhao, Liping; Koslovsky, Janet; Reinhard, Jutta; Bähler, Martin; Witt, Axel; Provance, David William; Mercer, John A.

doi:10.1073/pnas.93.20.10826

Cited by 81 publications

(75 citation statements)

References 38 publications

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“…6 MYO5B is a large gene (372 kb, 40 exons) encoding a multifunctional protein expressed in a variety of brain structures, including dendritic spines, at high levels. 39,40 Myosins are known to transport proteins in neurons through binding to their C-terminal globular domain, 41 and MYO5B specifically regulates vesicle trafficking. 42 Strikingly, MYO5B has been shown to regulate EGFR cycling in both canine (MDCK) and human cells (A431).…”

Section: Discussionmentioning

confidence: 99%

Whole-genome association study of bipolar disorder

et al. 2008

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We performed a genome-wide association scan in 1461 patients with bipolar (BP) 1 disorder, 2008 controls drawn from the Systematic Treatment Enhancement Program for Bipolar Disorder and the University College London sample collections with successful genotyping for 372 193 single nucleotide polymorphisms (SNPs). Our strongest single SNP results are found in myosin5B (MYO5B; P = 1.66 Â 10 À7 ) and tetraspanin-8 (TSPAN8; P = 6.11 Â 10 À7 ). Haplotype analysis further supported single SNP results highlighting MYO5B, TSPAN8 and the epidermal growth factor receptor (MYO5B; P = 2.04 Â 10 À8 , TSPAN8; P = 7.57 Â 10 À7 and EGFR; P = 8.36 Â 10 À8 ). For replication, we genotyped 304 SNPs in family-based NIMH samples (n = 409 trios) and University of Edinburgh case-control samples (n = 365 cases, 351 controls) that did not provide independent replication after correction for multiple testing. A comparison of our strongest associations with the genome-wide scan of 1868 patients with BP disorder and 2938 controls who completed the scan as part of the Wellcome Trust Case-Control Consortium indicates concordant signals for SNPs within the voltage-dependent calcium channel, L-type, alpha 1C subunit (CACNA1C) gene. Given the heritability of BP disorder, the lack of agreement between studies emphasizes that susceptibility alleles are likely to be modest in effect size and require even larger samples for detection.

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Section: Discussionmentioning

confidence: 99%

Whole-genome association study of bipolar disorder

et al. 2008

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“…It would be worthwhile to know what kind of functional relationship may exist between these neurochemical characteristics of the dorsal cochlear nuclei and the coincident MVa expression. Zhao et al (1996) demonstrated that another member of the myosin V class, the so-called myr 6 (myosin Vb), is specifically expressed in hippocampus, choroids plexus and amygdala of normal rats. Consequently, this paralogue may be a candidate to probe for the participation of brain myosins in temporal lobe hyperexcitable networks.…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical localization of myosin va in the adult rat brain

et al. 2003

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Abstract-Brain myosin Va (MVa) is a molecular motor associated with plastic changes during development. MVa has previously been detected in the cell body and in dendrites of neuronal cells in culture, in cells of the guinea-pig cochlea, as well as in cerebellar cells. Adult Wistar rats (n‫,)41؍‬ 250 -300 g, were perfused with standard methods for immunohistochemistry, using a polyclonal, affinity-purified rabbit antibody against MVa tail domain. Anti-MVa antibody specifically stained neuronal nuclei from forebrain to cerebellar regions, and more intensely sensory nuclei. Differences in MVa immunoreactivity were detected between brain nuclei, ranging from very intense to weak staining. The analysis of MVa and glial fibrillary acidic protein staining in adjacent brain sections demonstrated a clear-cut neuronal labeling rather than an astroglial staining. The studies presented here represent a comprehensive map of MVa regional distribution in the CNS of the adult rat and may contribute to the basic understanding of its role in brain function and plasticity, particularly in relationship to phenomena that involve molecular motors, such as neurite outgrowth, organelle transport and neurotransmitter-vesicle cycling. It is important to highlight that this is a pioneer immunohistochemical study on the distribution of MVa on the whole brain of adult rats, a first step toward the understanding of its function in the CNS.

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“…There are three myosin V family members in mammals (Mercer et al, 1991;Zhao et al, 1996;Rodriguez and Cheney, 2002) that have been implicated in genetic diseases and membrane trafficking (Mooseker and Cheney, 1995;Hasson and Mooseker, 1996;Titus, 1997). A role for myosin Vb in endocytic trafficking, specifically at a recycling step (Lapierre et al, 2001;Volpicelli et al, 2002), has been suggested based on the ability of the myosin Vb tail domain to retard recycling of the M4 muscarinic acetylcholine receptor (Volpicelli et al, 2002) and the Tf receptor (Lapierre et al, 2001) and to result in the accumulation of Tf in pericentriolar vesicles (but see Provance et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

CART: An Hrs/Actinin-4/BERP/Myosin V Protein Complex Required for Efficient Receptor Recycling

Yan

Wei

Kujala

et al. 2005

MBoC

115

121

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Altering the number of surface receptors can rapidly modulate cellular responses to extracellular signals. Some receptors, like the transferrin receptor (TfR), are constitutively internalized and recycled to the plasma membrane. Other receptors, like the epidermal growth factor receptor (EGFR), are internalized after ligand binding and then ultimately degraded in the lysosome. Routing internalized receptors to different destinations suggests that distinct molecular mechanisms may direct their movement. Here, we report that the endosome-associated protein hrs is a subunit of a protein complex containing actinin-4, BERP, and myosin V that is necessary for efficient TfR recycling but not for EGFR degradation. The hrs/actinin-4/BERP/myosin V (CART [cytoskeleton-associated recycling or transport]) complex assembles in a linear manner and interrupting binding of any member to its neighbor produces an inhibition of transferrin recycling rate. Disrupting the CART complex results in shunting receptors to a slower recycling pathway that involves the recycling endosome. The novel CART complex may provide a molecular mechanism for the actin-dependence of rapid recycling of constitutively recycled plasma membrane receptors. INTRODUCTIONEndocytosis is required for the uptake of essential nutrients from the extracellular environment as well as for retrieval of proteins and lipids that are added to the plasma membrane during fusion of regulated and constitutive secretory vesicles (De Camilli and Takei, 1996;Koenig and Ikeda, 1996;Robinson et al., 1996;Mukherjee et al., 1997;Schmid, 1997;Betz and Angleson, 1998;Koenig et al., 1998;Stoorvogel, 1998;D'Hondt et al., 2000;Gruenberg, 2001). The endocytic pathway can be separated into numerous stages based on the movement of cargo and the identification of morphologically defined compartments (De Camilli and Takei, 1996;Koenig and Ikeda, 1996;Robinson et al., 1996;Mukherjee et al., 1997;Schmid, 1997;Betz and Angleson, 1998;Koenig et al., 1998;Stoorvogel, 1998;D'Hondt et al., 2000;Gruenberg, 2001). Early events in the endocytic process include membrane invagination and vesicle budding from the plasma membrane, formation of transport vesicles, and fusion with early endosomes. Later events include cargo sorting, and additional transport/fusion steps, including those responsible for transport to the lysosome for degradation, and those responsible for recycling back to various compartments (De Camilli and Takei, 1996;Koenig and Ikeda, 1996;Robinson et al., 1996;Mukherjee et al., 1997;Schmid, 1997;Betz and Angleson, 1998;Koenig et al., 1998;Stoorvogel, 1998;D'Hondt et al., 2000;Gruenberg, 2001). Although much progress has been made in elucidating the molecular processes involved in early endocytic events, such as those involved in the genesis of clathrin-coated endocytic transport vesicles, an equally clear understanding of later events remains elusive.The early endosome is a crucial point in the endocytic pathway to sort cargo for transport to late endosomes for eventual degradation in the...

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Cloning and characterization of myr 6, an unconventional myosin of the dilute/myosin-V family.

Cited by 81 publications

References 38 publications

Whole-genome association study of bipolar disorder

Whole-genome association study of bipolar disorder

Immunohistochemical localization of myosin va in the adult rat brain

CART: An Hrs/Actinin-4/BERP/Myosin V Protein Complex Required for Efficient Receptor Recycling

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