Cloning and characterization of mouse Klk27, a novel tissue kallikrein expressed in testicular Leydig cells and exhibiting chymotrypsin‐like specificity

Matsui, Hitoshi; Moriyama, Akihiko; Takahashi, Takayuki

doi:10.1046/j.1432-1033.2000.01786.x

Cited by 22 publications

(14 citation statements)

References 37 publications

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“…These values are larger than those calculated from the amino acid sequences of these proteins. Such differences in molecular size between the theoretical and experimental values have previously been reported for other proteins, including serine proteinase family members [11,12,18,36]. Interestingly, the testicular tissues in the present study contained Gzmn in two forms (M r ϭ 31 kDa and 30 kDa).…”

Section: Discussionsupporting

confidence: 65%

“…At present, 11 Gzm genes have been identified for the mouse, including granzyme L (Gzml), which is presumed to be a pseudogene with early termination sites [3]. Our current data regarding RT-PCR analysis for the 10 functional Gzm species revealed that in addition to Gzmn and Gzmb, four other genes (Gzma, Gzmc, Gzmk, and Gzmm) are expressed in the mouse testes (Figs.…”

Section: Discussionmentioning

confidence: 86%

“…(Stratagene) with 5 g of poly(A) ϩ RNA and was packaged using Gigapack III extract (Stratagene). Screening was conducted for approximately 1 ϫ 10 6 plaques from the library using the 448-bp EcoRV fragment according to the procedure previously described [11,12]. This resulted in a new full-length granzyme clone of 975 bp.…”

Section: Cdna Cloning Of Mouse Gzmnmentioning

confidence: 99%

“…In situ hybridization studies were performed as described previously [11]. A full-length Gzmn cDNA (975 bp) was used to generate both antisense and sense digoxigenin (DIG)-labeled riboprobes with a DIG RNA Labeling Mix (Roche Diagnostics, Mannheim, Germany).…”

Section: In Situ Hybridization Analysismentioning

confidence: 99%

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Granzyme N, a Novel Granzyme, Is Expressed in Spermatocytes and Spermatids of the Mouse Testis1

Takano¹,

Matusi²,

Takahashi³

2004

Biology of Reproduction

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We cloned a cDNA for a novel granzyme, granzyme N (Gzmn), from a mouse testes cDNA library. The testes contained two distinct species of Gzmn mRNA, one of which codes for a complete protein of 248 amino acids with three essential residues required for catalytic activity. The Gzmn mRNA was specifically expressed in the testes of adult mice. The Gzmn expression was found to initiate in the testes at 3 wk of age and to become more prominent as the animal reached sexual maturity. In situ hybridization analysis revealed that both spermatocytes and spermatids of the adult mouse testes express Gzmn mRNA. Consistent with these findings, the protein was immunohistochemically detected in the spermatocytes and spermatids, although some of the germ cells showed no positive staining. Gzmn was demonstrated to be a secretory and N-glycosylated protein that exists in two protein forms in the testes extract. In the cryptorchid testes, the expression of Gzmn transcript was drastically reduced on Postoperative Day 10, whereas the protein level was gradually decreased starting on Day 6. The local heating (43 degrees C, 20 min) of the testes did not change the Gzmn expression level at either 8 or 16 h after treatment. These results suggest that Gzmn is not involved in the process of germ cell apoptosis induced by heat shock, but that it may be involved in spermatogenesis in the mouse testes.

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Section: Discussionsupporting

confidence: 65%

Section: Discussionmentioning

confidence: 86%

Section: Cdna Cloning Of Mouse Gzmnmentioning

confidence: 99%

Section: In Situ Hybridization Analysismentioning

confidence: 99%

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Granzyme N, a Novel Granzyme, Is Expressed in Spermatocytes and Spermatids of the Mouse Testis1

Takano¹,

Matusi²,

Takahashi³

2004

Biology of Reproduction

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“…Kallikrein 24 has been shown to be androgen but not estrogen regulated in vitro (45), and the expression of these three kallikreins has also been found to be reduced in the testis of AR mutant mice (42). In the testis, kallikreins 21, 24, and 27 are exclusively expressed in the Leydig cells, and their expressions are shown to increase during development, being highest at the age of 8 wk (45)(46)(47). Thus, they can be considered as adult-type Leydig cell markers.…”

Section: W T a R O M + E R K O A R O M + X E R K O B E R K Omentioning

confidence: 99%

Increased Exposure to Estrogens Disturbs Maturation, Steroidogenesis, and Cholesterol Homeostasis via Estrogen Receptor α in Adult Mouse Leydig Cells

Strauss¹,

Kallio²,

Desai³

et al. 2009

Endocrinology

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Deteriorated male reproductive health has been connected to overexposure to estrogens or to imbalanced androgen-estrogen ratio. Transgenic male mice expressing human aromatase (AROM ϩ mice) serve as an apt model for the study of the consequences of an altered androgenestrogen ratio. Our previous studies with AROM ϩ mice showed that low androgen levels together with high estrogen levels result in cryptorchidism and infertility. In the present study, the AROM ϩ mice were shown to have severe abnormalities in the structure and function of Leydig cells before the appearance of spermatogenic failure. Decreased expression of adult-type Leydig cell markers (Ptgds, Vcam1, Insl3, Klk21, Star, Cyp17a1, and Hsd17b3) indicated an immature developmental stage of the Leydig cells, which appears to be the first estrogen-dependent alteration. Genes involved in steroidogenesis (Star, Cyp17a1, and Hsd17b3) were suppressed despite normal LH levels. The low expression level of kallikreins 21, 24, and 27 potentially further inhibited Leydig cell function via remodeling extracellular matrix composition. In connection with disrupted steroidogenesis, Leydig cells showed enlarged mitochondria, a reduced amount of smooth endoplasmic reticulum, and an accumulation of cholesterol and precursors for cholesterol synthesis. The results of studies with AROM ϩ mice crossed with estrogen receptor ␣ or ␤ (ER␣ and ER␤, respectively) knockout mice lead to the conclusion that the structural and functional disorders caused by estrogen exposure were mediated via ER␣, whereas ER␤ was not involved. (Endocrinology 150: [2865][2866][2867][2868][2869][2870][2871][2872] 2009)

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Increased oxidized low‐density lipoprotein in mice exposed to a high‐fat diet impaired spermatogenesis by inhibiting testosterone synthesis via the Klk1bs/Eid3 pathway

Yuan,

Hong,

Wang

et al. 2024

Clinical & Translational Med

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Cloning and characterization of mouse Klk27, a novel tissue kallikrein expressed in testicular Leydig cells and exhibiting chymotrypsin‐like specificity

Cited by 22 publications

References 37 publications

Granzyme N, a Novel Granzyme, Is Expressed in Spermatocytes and Spermatids of the Mouse Testis1

Granzyme N, a Novel Granzyme, Is Expressed in Spermatocytes and Spermatids of the Mouse Testis1

Increased Exposure to Estrogens Disturbs Maturation, Steroidogenesis, and Cholesterol Homeostasis via Estrogen Receptor α in Adult Mouse Leydig Cells

Increased oxidized low‐density lipoprotein in mice exposed to a high‐fat diet impaired spermatogenesis by inhibiting testosterone synthesis via the Klk1bs/Eid3 pathway

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