Cloning and characterization of genes encoding trehalose-6-phosphate synthase (TPS1) and trehalose-6-phosphate phosphatase (TPS2) from

Abstract

In many organisms, trehalose protects against several environmental stresses, such as heat, desiccation, and salt, probably by stabilizing protein structures and lipid membranes. Trehalose synthesis in yeast is mediated by a complex of trehalose-6-phosphate synthase (TPS1) and trehalose-6-phosphate phosphatase (TPS2). In this study, genes encoding TPS1 and TPS2 were isolated from Zygosaccharomyces rouxii (designated ZrTPS1 and ZrTPS2, respectively). They were functionally identified by their complementation of the tps1 and tps2 yeast deletion mutants, which are unable to grow on glucose medium and with heat, respectively. Full-length ZrTPS1 cDNA is composed of 1476 nucleotides encoding a protein of 492 amino acids with a molecular mass of 56 kDa. ZrTPS2 cDNA consists of 2843 nucleotides with an open reading frame of 2700 bp, which encodes a polypeptide of 900 amino acids with a molecular mass of 104 kDa. The amino acid sequence encoded by ZrTPS1 has relatively high homology with TPS1 of Saccharomyces cerevisiae and Schizosaccharomyces pombe, compared with TPS2. Western blot analysis showed that the antibody against S. cerevisiae TPS1 recognizes ZrTPS1. Under normal growth conditions, ZrTPS1 and ZrTPS2 were highly and constitutively expressed, unlike S. cerevisiae TPS1 and TPS2. Salt stress and heat stress reduced the expression of the ZrTPS1 and ZrTPS2 genes, respectively.

Inferring Gene Family Histories in Yeast Identifies Lineage Specific Expansions

“…In C. albicans it has been shown that trehalose mobilisation is important for tolerance of hydrogen peroxide [54]. Z. rouxii is well known to be tolerant to salt and osmotic stress [55], [56] and it has been suggested that the high expression of trehalose synthesis genes under non-stress conditions [57] may be important in a range of stress responses [58]. It may be the case that Z. rouxii ' s naturally high rate of trehalose synthesis makes the species tolerant of a wide range of stresses and the expansion of a family associated with hydrogen peroxide resistance is a complementary adaptation to a specific environmental stress.…”

Cloning and pattern of expression of trehalose-6-phosphate synthase cDNA from Catantops pinguis (Orthoptera: Catantopidae)

“…Trehalose has a dual function as a storage carbohydrate and a stress protection metabolite (Kwon et al, 2003;Thevelein, 1996). Transcriptional levels of TPS1 and TPS2 are increased by heat shock, cold or ethanol stress, salt stress, nutrient starvation, and other osmotic stresses (Winderickx et al, 1996;Wolschek & Kubicek, 1997;Soto et al, 2002;Pereira et al, 2001).…”

Enhancement of the biocontrol agent Candida oleophila (strain O) survival and control efficiency under extreme conditions of water activity and relative humidity

“…Therefore, numerous researchers reported that the stress tolerance of microorganisms could be improved by inducing the accumulation of intracellular sugars (Teixidõ et al, 1998a,b). Trehalose, a non-reducing disaccharide and a major reserve carbohydrate, has been shown to be effective as a protectant metabolite against environmental stresses, such as heat, dehydration, freezing and hyperosmotic stress (Kown et al, 2003).…”