Cloning and characterization of dRFX, the Drosophila member of the RFX family of transcription factors

Durand, Bénédicte; Vandaele, Camille; Spencer, Doran; Pantalacci, Sophie; Couble, Pierre

doi:10.1016/s0378-1119(00)00093-7

Cited by 30 publications

(41 citation statements)

References 30 publications

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“…The complete Rfx 3.9kb cDNA (Durand et al, 2000) was cloned in pUAST vector and subsequently injected in flies, as described (Spradling, 1986). Both GAL4 drivers, P{GawB}elav C155 and P{GAL4}sca , which express the protein in the entire nervous system (Luo et al, 1994) and in the peripheral nervous system (Guo et al, 1996) Rescued flies show a complete reversal of the uncoordinated phenotype and normal viability and reversal of the mutant phenotype was analyzed for the dendrite morphology by GFP labeling in adult legs and wings.…”

Section: Rfx Rescuementioning

confidence: 99%

“…RFX 253 or RFX protein was produced by in vitro transcription and translation. EMSA was performed as previously described (Durand et al, 2000).…”

Section: Sequencing and Electromobility Shift Assay (Emsa)mentioning

confidence: 99%

“…Close observation of larvae show a peculiar foraging behavior with weak food digging efficiency. This could result from an altered peripheral sensitivity, a hypothesis that was suggested by the fact that Rfx is expressed in sensory neurons during embryogenesis (Vandaele et al, 2000). We thus decided to investigate the gustatory and olfactory-driven behavior of mutant larvae.…”

Section: Rfx Larvae Are Insensitive To Chemical or Odorant Stimulimentioning

confidence: 99%

“…Mammalian RFX5 is essential for the transcription of MHC class II genes in the immune response (for a review, see Reith and Mach, 2001), but little is known about the cellular functions of the other mammalian RFX proteins. Two Rfx genes can be identified in Drosophila (Durand et al, 2000) (FlyBase: http://flybase.harvard.edu:7081/). Rfx is homologous to daf-19 and to mammalian Rfx1, Rfx2 and Rfx3, whereas the second gene shares conserved motifs with Rfx5, the most divergent mammalian Rfx (A. L., unpublished).…”

Section: Introductionmentioning

confidence: 99%

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DrosophilaRegulatory factor X is necessary for ciliated sensory neuron differentiation

et al. 2002

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Section: Rfx Rescuementioning

confidence: 99%

“…RFX 253 or RFX protein was produced by in vitro transcription and translation. EMSA was performed as previously described (Durand et al, 2000).…”

Section: Sequencing and Electromobility Shift Assay (Emsa)mentioning

confidence: 99%

Section: Rfx Larvae Are Insensitive To Chemical or Odorant Stimulimentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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DrosophilaRegulatory factor X is necessary for ciliated sensory neuron differentiation

et al. 2002

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“…That the sensory mechanism localizes to the cilium is supported by the fact that mutations in genes that affect JO ciliary development and morphology lead to deafness. These include components of intraflagellar transport (IFT) such as the anterograde kinesin motor subunits kinesin-like protein 64D (Klp64D) and the kinesin associated protein (DmKAP) (Sarpal et al, 2003), the retrograde cytoplasmic dynein motor encoded by beethoven (btv) (Eberl et al, 2000, Sharma et al, in preparation), the anterograde IFT-B particle proteins no mechanoreceptor potential B (nompB) (Han et al, 2003), outer segment protein 2 (oseg2; also called osm-1) and outer segment protein 5 (oseg5) (Avidor-Reiss et al, 2004), the retrograde IFT-A particle proteins oseg1 and reduced mechanoreceptor potential A (rempA; also called oseg3) (Avidor-Reiss et al, 2004, Lee et al, in preparation) and the ciliary transcription regulator Drosophila regulatory factor X (dRfx) (Durand et al, 2000, Dubruille et al, 2002. Mutations that appear to affect the ciliary axoneme also lead to deafness.…”

Section: How the Drosophila Antenna Work As An Earmentioning

confidence: 99%

Development of Johnston's organ in Drosophila

Eberl¹,

Boekhoff-Falk²

2007

Int. J. Dev. Biol.

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Hearing is a specialized mechanosensory modality that is refined during evolution to meet the particular requirements of different organisms. In the fruitfly, Drosophila, hearing is mediated by Johnston's organ, a large chordotonal organ in the antenna that is exquisitely sensitive to the near-field acoustic signal of courtship songs generated by male wing vibration. We summarize recent progress in understanding the molecular genetic determinants of Johnston's organ development and discuss surprising differences from other chordotonal organs that likely facilitate hearing. We outline novel discoveries of active processes that generate motion of the antenna for acute sensitivity to the stimulus. Finally, we discuss further research directions that would probe remaining questions in understanding Johnston's organ development, function and evolution.

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Regulatory factor X2 (RFX2) binds to the H1t/TE1 promoter element and activates transcription of the testis‐specific histone H1t gene

Wolfe

Wilkerson

Prado

et al. 2003

J of Cellular Biochemistry

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Transcription of the mammalian testis-specific linker histone H1t gene occurs only in pachytene primary spermatocytes during spermatogenesis. Studies of the wild type (Wt) and mutant H1t promoters in transgenic mice show that transcription of the H1t gene is dependent upon the TE promoter element. We purified an 85 kDa protein from rat testis nuclear extracts using the TE1 subelement as an affinity chromatography probe and analysis revealed that the protein was RFX2. The TE1 element is essentially an X-box DNA consensus element and regulatory factor X (RFX) binds specifically to this element. Polyclonal antibodies directed against RFX2 supershift the low mobility testis nuclear protein complex formed in electrophoretic mobility shift assays (EMSA). RFX2 derived from primary spermatocytes, where the transcription factor is relatively abundant, binds with high affinity to the TE1 element. Coexpression of RFX2 together with an H1t promoter/reporter vector activates the H1t promoter in a cultured GC-2spd germinal cell line, but mutation of either the TE1 subelement or the TE2 subelements represses activity. These observations lead us to conclude that the TE1 and TE2 subelements of the testis-specific histone H1t promoter are targets of the transcription factor RFX2 and that this factor plays a key role in activating transcription of the H1t gene in primary spermatocytes. Published 2003 Wiley-Liss, Inc.

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Cloning and characterization of dRFX, the Drosophila member of the RFX family of transcription factors

Cited by 30 publications

References 30 publications

DrosophilaRegulatory factor X is necessary for ciliated sensory neuron differentiation

DrosophilaRegulatory factor X is necessary for ciliated sensory neuron differentiation

Development of Johnston's organ in Drosophila

Regulatory factor X2 (RFX2) binds to the H1t/TE1 promoter element and activates transcription of the testis‐specific histone H1t gene

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