Cloning and characterization of different human sequences related to the onc gene (v-myc) of avian myelocytomatosis virus (MC29).

Abstract

We have studied the genomic organization of human cellular sequences (c-myc) homologous to the transforming gene (v-myc) of avian myelocytomatosis virus (MC29). Southern blotting experiments using v-myc probes showed that several fragments of the human genome contain sequences related to the central part of v-myc but only few of them are homologous to the 3' portion of the viral gene. Several recombinant phages which represent different regions of the genome containing c-myc-related sequences were isolated from a human DNA library. Two clones (lambda-LMC-12 and -41) overlap over approximately 17 kilobases of DNA where a sequence homologous to that of the entire v-myc is present. Restriction mapping experiments and heteroduplex analysis show that c-myc sequences of this locus are interrupted by one intron, suggesting that lambda-LMC-12 and -41 contain the complete functional c-myc gene. Three other clones (lambda-LMC-3, -4, and -26) do not overlap and contain sequences related to only approximately 0.3 kilobase of v-myc but lack 5' and 3' portions of the gene. These sequences are not interrupted by introns and are more divergent from v-myc than is the complete gene, suggesting that they may represent either pseudogenes or parts of distantly related genes.

Structure and expression of the oncogene c-myc in fresh tumor material from patients with hematopoietic malignancies.

“…The restriction map of XhR1-15 shown in Fig. 1 appears nearly identical to that described by Dalla-Favera et al (12) and is consistent with the human c-myc nucleotide sequence (2,9). Additional restriction sites in the human genome surrounding the cloned fragment were determined and are also shown in Fig.…”

“…1). These other sequences, which have homology to the 5' half of v-myc, have been suggested to be pseudogenes (12). Only RNA transcribed from the full-length locus will be detected with the ClaI-EcoRI probe.…”

Failure of central nervous system myelination in MBP/c-myc transgenic mice: evidence for c-myc cytotoxicity

“…To construct the MBP/c-myc transgene, a KpnI ± XbaI fragment containing the 3 kb MBP promoter was inserted in the 5' orientation at the KpnI ± XbaI site of a genomic clone of the human c-myc gene (Dalla-Favera et al, 1982). In order to insure high levels of expression of the c-myc transgene, exon 1, which contains negative regulatory elements, was removed (Xu et al, 1991).…”

c-MYC activates protein kinase A (PKA) by direct transcriptional activation of the PKA catalytic subunit beta (PKA-Cβ) gene

“…Vimentin probe was kindly provided by R Liem. c-MYC exon 3 and exon 1 probes were obtained from the pMC41RC and pMC41ER plasmids, respectively (Dalla-Favera et al, 1982). The SV40 poly(A) tail probe and human b-actin probe were previously described (Wu et al, 1999b).…”