Cloning and characterization of cell strains derived from human corneal stroma and sclera

Kashiwagi, Yoshiko; Nishitsuka, Koichi; Namba, Hiroyuki; Kamiryo, Masaru; Takamura, Hiroshi; Yamashita, Hidetoshi

doi:10.1007/s10384-009-0749-5

Cited by 5 publications

(5 citation statements)

References 24 publications

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“…The establishment of primary cell lines from mesenchymal tissue has been reported with the use of human papilloma virus early function genes 21,22. The stem-like stromal cells of GCT propagate in culture and maintain their phenotype for several passages and can be studied in vitro in a manner similar to that of established and commercially available cell lines.…”

Section: Discussionmentioning

confidence: 99%

Gene transfection in primary stem-like cells of giant cell tumor of bone

Singh¹,

Mak²,

Power³

et al. 2010

SCCAA

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The neoplastic stem-like stromal cell of giant cell tumor of bone (GCT) survives for multiple passages in primary culture with a stable phenotype, and exhibits multipotent characteristics. The pathophysiology of this tumor has been studied through the primary culture of these cells. However, successful gene transfer of these cells has not been reported to date. In this short report, we describe the development of the first reported technique that results in efficient gene transfection in primary stem-like cells of GCT.

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Section: Discussionmentioning

confidence: 99%

Gene transfection in primary stem-like cells of giant cell tumor of bone

Singh¹,

Mak²,

Power³

et al. 2010

SCCAA

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“…It is, therefore, believed that mRNA expression of HAS in the three cell strains was not caused by TGF-b1 and PDGF-BB. We reported previously that HA production and HAS2 mRNA expression were increased by TGF-b1 and PDGF-BB treatment in human corneal stroma-derived and scleraderived cells [16]. Regulation of HA production and HAS2 mRNA expression by TGF-b1 and PDGF-BB treatment in human conjunctiva-derived cells is different from that of human corneal stroma-derived and sclera-derived cells.…”

Section: Discussionmentioning

confidence: 95%

“…In a variety of cellular systems, alterations in HA biosynthesis correlate with increases in HAS mRNA expression [11][12][13][14]. HAS gene and protein expression are differentially regulated by several cytokines and growth factors related to wound healing, for example transforming growth factor-b (TGF-b) and platelet-derived growth factor-BB (PDGF-BB) [11,12,[15][16][17][18][19][20][21][22][23]. In the conjunctiva, HAS1 protein expression has been documented in monkeys and cows [24,25].…”

Section: Introductionmentioning

confidence: 99%

Hyaluronan production and hyaluronan synthase expression in three human conjunctiva-derived cell strains

et al. 2011

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“…GCT mesenchymal stromal cells became the homogeneous cell type whereas the multinucleated giant cells were eliminated from culture and maintained the phenotype up to the tenth passage were used for experiments [19, 20]. Here, we are describing gene transfer and stable cells generation in patient-derived primary GCT stromal cells.…”

Section: Discussionmentioning

confidence: 99%

Expressional Analysis of GFP-Tagged Cells in an In Vivo Mouse Model of Giant Cell Tumor of Bone

Singh

Singh²,

Mak³

et al. 2013

TOORTHJ

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Giant cell tumor of bone in a neoplastic stromal cell which survives for multiple passages in primary cell culture with a stable phenotype. In the pathological environment of GCT, the neoplastic nature of the mesenchymal stromal component drives local hematopoietic precursors to undergo fusion and form multinucleated osteoclast like giant cells. There is currently very limited knowledge about the pathogenesis of GCT due to the lack of suitable in vivo models for this tumor. Here we report stable gene transfer of Green fluorescence protein (GFP) in GCT stromal cells. In the present study, we have used GCT stromal cells that stably express enhanced green fluorescence protein (GFP) that are used in a new in vivo culture model. Our results show the utility of the GFP tagged cell lines that stably express GFP signals up to 52 weeks of continuous growth. The in vivo model described herein can serve as an excellent system for in vivo therapeutic and mechanistic evaluation of existing and novel targets for GCT.

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Cloning and characterization of cell strains derived from human corneal stroma and sclera

Abstract: Cell strains derived from corneal stroma and sclera fibroblast cells can be established using HPV16 E6/E7 immortalized genes of the same origin. The phenotypic cell characteristics did not change after transfection, immortalization, or successive passages in culture.

Cited by 5 publications

References 24 publications

Gene transfection in primary stem-like cells of giant cell tumor of bone

Gene transfection in primary stem-like cells of giant cell tumor of bone

Hyaluronan production and hyaluronan synthase expression in three human conjunctiva-derived cell strains

Expressional Analysis of GFP-Tagged Cells in an In Vivo Mouse Model of Giant Cell Tumor of Bone

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