Cloning and characterization of cDNAs encoding the ϵ-subunit of eukaryotic initiation factor-2B from rabbit and human

Asuru, Agatha I.; Mellor, Harry; Thomas, N. Shaun B.; Yu, Lu; Chen, Jane Jane; Crosby, John S.; Hartson, Steven D.; Kimball, Scot R.; Jefferson, Leonard S.; Matts, Robert L.

doi:10.1016/0167-4781(96)00054-1

Cited by 2 publications

(3 citation statements)

References 33 publications

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“…The second distinguishing feature of BZAP45 is its C-terminal nucleotide binding fold. This region exhibits a high degree of similarity with domains present in eukaryotic translation initiation factors (eIFs) that are highly conserved between yeast and human ( − ). The nucleotide binding fold of BZAP45 is most similar to that of eIF-5 and the Epsilon subunit of eIF-2B ( − ) which function as GDP−GTP exchange factors.…”

Section: Discussionmentioning

confidence: 99%

“…This region exhibits a high degree of similarity with domains present in eukaryotic translation initiation factors (eIFs) that are highly conserved between yeast and human ( − ). The nucleotide binding fold of BZAP45 is most similar to that of eIF-5 and the Epsilon subunit of eIF-2B ( − ) which function as GDP−GTP exchange factors. The presence of the bZIP domain and nucleotide binding fold suggests that BZAP45 is a natural chimera of domains derived from two distinct classes of proteins.…”

Section: Discussionmentioning

confidence: 99%

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Purification and Functional Analysis of a Novel Leucine-Zipper/Nucleotide-Fold Protein, BZAP45, Stimulating Cell Cycle Regulated Histone H4 Gene Transcription

et al. 2001

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Regulation of histone gene transcription at the G1/S phase transition via the Site II cell cycle control element is distinct from E2F-dependent mechanisms operative at the growth factor-related restriction point. E2F-independent activation of histone H4 gene expression combines contributions of several promoter factors, including HiNF-M/IRF2 and the HiNF-D/CDP-cut complex which contains pRB, CDK1, and cyclin A as non-DNA binding subunits. Mutational analyses suggest additional rate-limiting factors for Site II function. Using sequence-specific Site II DNA affinity chromatography, we identified a 45 kDa protein (KIAA0005 or BZAP45) that is embryonically expressed and phylogenetically conserved. Based on amino acid sequence analysis, BZAP45 contains a unique decapeptide that is part of a putative leucine-zipper protein with a nucleotide (ATP or GTP) binding fold. Bacterial expression of a full-length cDNA produces a 45 kDa protein. Binding studies reveal that highly purified BZAP45 does not interact with Site II, suggesting that BZAP45 function may require partner proteins. Forced expression of BZAP45 strongly stimulates H4 promoter (nt -215 to -1)/CAT reporter gene activity. Deletion analyses and point mutations indicate that BZAP45 enhances H4 gene transcription through Site II. Thus, BZAP45 is a novel regulatory factor that contributes to transcriptional control at the G1/S phase transition.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Purification and Functional Analysis of a Novel Leucine-Zipper/Nucleotide-Fold Protein, BZAP45, Stimulating Cell Cycle Regulated Histone H4 Gene Transcription

et al. 2001

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“…EIF2B5. Although EIF2B5 is not involved in DNA repair per se, it has a role in the initiation of translation (49). A single patient has been reported so far with ovarioleukodystrophy and a compound heterozygosity for EIF2B5 (50).…”

Section: Genes Involved In Dna Repairmentioning

confidence: 99%

Whole exome sequencing in a cohort of familial premature ovarian insufficiency cases reveals a broad array of pathogenic or likely pathogenic variants in 50% of families

Rogers¹,

Kerlan²,

Delemer³

et al. 2022

Fertility and Sterility

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Cloning and characterization of cDNAs encoding the ϵ-subunit of eukaryotic initiation factor-2B from rabbit and human

Cited by 2 publications

References 33 publications

Purification and Functional Analysis of a Novel Leucine-Zipper/Nucleotide-Fold Protein, BZAP45, Stimulating Cell Cycle Regulated Histone H4 Gene Transcription

Purification and Functional Analysis of a Novel Leucine-Zipper/Nucleotide-Fold Protein, BZAP45, Stimulating Cell Cycle Regulated Histone H4 Gene Transcription

Whole exome sequencing in a cohort of familial premature ovarian insufficiency cases reveals a broad array of pathogenic or likely pathogenic variants in 50% of families

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