Abstract:An ascorbate peroxidase (APX) cDNA, designated MaAPX1, was isolated from banana fruit by suppression subtractive hybridization (SSH). MaAPX1 shares an extensive sequence identity (79 to 83%) with other plant APX homologues. Southern blot analysis revealed only two copies of the APX gene in the banana genome. Reverse-transcriptase PCR analysis of MaAPX1 expression confirmed its expression in the root, leaf, flower and fruit, with higher levels detected in the leaf compared to other organs. Real-time quantitativ… Show more
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