Cloning and Characterization of an Anaerobically Induced cDNA Encoding Glucose-6-Phosphate Isomerase from Maize

Abstract

Anaerobic stress in maize (Zea mays L.) leads to a drastic alteration in gene expression and results in the selective synthesis of a small number of polypeptides named anaerobic proteins (Sachs et al., 1980). Other plants studied showed similar responses when subjected to an anaerobic environment (Russell et al., 1990). To date, the majority of the anaerobic proteins that have been identified were found to be enzymes involved in the glycolytic pathway or Glc-P metabolism. Recently, however, Peschke and Sachs (1994) reported two anaerobically induced transcripts that do not encode enzymes involved in Glc metabolism and apparently serve other roles in the response of maize to flooding stress. The expression of the anaerobic proteins has been proposed to be regulated at the levels of both transcription and translation (Sachs et al., 1980; Hake et al., 1985). GPI (EC 5.3.1.9) is an essential glycolytic enzyme that catalyzes the reversible isomerization of Glc-6-P and Fru-6-P, a reaction that also immediately precedes Suc biosynthesis in plants. This enzyme has also been called phosphohexose isomerase and phophoglucose isomerase. Two isozyme forms of GPI, one localized in the cytosol and the other in the plastid, have been reported in other plants and may also exist in maize (Weeden and Gottlieb, 1982; Kelley and Freeling, 1984). In general, plants examined contain a single gene for GPI-C, with the exception of Clarkia, which has two copies, resulting from gene duplication within that species (Weeden and Gottlieb, 1979). Three forms of GM have been reported in maize (Salamini et al., 1972). GPI-I encoded by the phil gene is expressed in both the devel

Sperm cells of Zea mays have a complex complement of mRNAs

“…Another, Zmsp131, encodes glucose‐6‐phosphate isomerase (GPI). Three forms of GPI have been reported in maize (Lal and Sachs, 1995; Salamini et al ., 1972). Because GPI catalyzes the second step in glycolysis, it is likely expressed in all the cells.…”

The introgression of a functional nuclear gene from Poa to Festuca ovina

“…We also examined Bromus sterilis, Poa supina, Poa trivialis, Poa chaixii, all diploid outcrossing species, plus Aira praecox, a diploid self-fertilizing species which according to Torrecilla et al (2004) falls in the same monophyletic clade as the fine-leaved fescues. All these species have 2nZ14 (Lid 1979 Lal & Sachs 1995), was used to construct primers for the initial amplification and sequencing of allele PgiC1 d from F. ovina. We used information from the PgiC1 genomic sequence from Clarkia lewisii (Thomas et al 1993) to infer intron/exon boundaries.…”

A DNA marker for the duplicated cytosolic PGI genes in sheep's fescue (Festuca ovina L.)

“…The published cDNA sequence for the cytosolic PGI gene in Zea mays (Lal & Sachs, 1995) was used to develop primer combinations that were tested on Festuca o ina material. Many, but not all, primer combinations led to the production of distinct DNA fragments, indicating a close homology between the PgiC genes in Zea and Festuca.…”