Cloning and characterization of a tuberous root-specific promoter from cassava (Manihot esculenta Crantz)

Putten, H.J.J. Koehorst-van; Wolters, Anne‐Marie A.; Pereira-Bertram, Isolde M.; Berg, Hans H. J. van den; Krol, Alexander R. van der; Visser, R.G.F.

doi:10.1007/s00425-012-1796-6

Cited by 17 publications

(11 citation statements)

References 30 publications

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“…These differences are probably because we were using Adira genotypes from BALITKABI, Indonesia instead of AM560-2 genotypes developed by CIAT, Columbia [45]. The same results were reported by other cassava researcher when the sequences were isolated from different genetic background [60]. Interestingly, MeEF1A3 promoter had 90% similarity to corresponding sequence at the scaffold 03015 from database.…”

Section: Discussionsupporting

confidence: 57%

Isolation and Characterization of Three Cassava Elongation Factor 1 Alpha (MeEF1A) Promoters

2014

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In plant genetic engineering, the identification of gene promoters leading to particular expression patterns is crucial for the development of new genetically modified plant generations. This research was conducted in order to isolate and characterize several new promoters from cassava (Manihot esculenta Crantz) elongation factor 1 alpha (EF1A) gene family. Three promoters MeEF1A3, MeEF1A4 and MeEF1A5 were successfully isolated. Sequence analyses showed that all of the promoters contain three conserved putative cis-acting elements which are located upstream of the transcription start site. These elements are included a TEF1, a TELO and TATA boxes. In addition, all of the promoters also have the 5′UTR intron but with a different lengths. These promoters were constructed translationally with gusA reporter gene (promoter::gusA fusion) in pBI-121 binary vector to build a new binary vector using Overlap Extension PCR Cloning (OEPC) technique. Transient expression assay that was done by using agroinfiltration method was used to show functionality of these promoters. Qualitative and quantitative analysis from GUS assay showed that these promoters were functional and conferred a specific activity in tobacco seedlings (Nicotiana tabacum), tomato fruits (Solanum lycopersicum) and banana fruits (Musa acuminata). We hypothesized that MeEF1A6 could be categorized as a constitutive promoter because it was able to drive the gene expression in all transformed tissue described in here and also comparable to CaMV35S. On the other hand, MeEF1A3 drove specific expression in the aerial parts of seedlings such as hypocotyl and cotyledon thus MeEF1A5 drove specific expression in fruit tissue. The results obtained from transient analysis showed that these promoters had a distinct activity although they came from same gene family. The DNA sequences identified here are new promoters potentially use for genetic engineering in cassava or other plants.

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Section: Discussionsupporting

confidence: 57%

Isolation and Characterization of Three Cassava Elongation Factor 1 Alpha (MeEF1A) Promoters

2014

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“…Recently, tissue-specific promoters have become the focus of plant genetic engineering. Researchers have isolated, cloned, and studied many tissue-specific promoters from different plants (Guo et al, 2010; Koehorst-van Putten et al, 2012; Chen et al, 2013; Zhu et al, 2014; Liu et al, 2015; Xue et al, 2018). The third type of promoter, the inducible promoter, is activated by abiotic or biotic stresses or hormone induction.…”

Section: Introductionmentioning

confidence: 99%

Identification and characterization of PsDREB2 promoter involved in tissue-specific expression and abiotic stress response from Paeonia suffruticosa

Liu

Zhu

Tan

et al. 2019

PeerJ

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Dehydration-responsive element-binding factor 2 (DREB2) belongs to the C-repeat-binding factor (CBF)/DREB subfamily of proteins. In this study, a 2,245 bp PsDREB2 promoter fragment was isolated from the genome of Paeonia suffruticosa. The fragment was rich in A/T bases and contained TATA box sequences, abscisic acid (ABA)-response elements, and other cis-elements, such as MYB and CAAT box. The promoter was fused with the β-glucuronidase (GUS) reporter gene to generate an expression vector. Arabidopsis thaliana was transformed with a flower dipping method. Gus activity in different tissues and organs of transgenic plants was determined via histochemical staining and quantified via GUS fluorescence. The activity of promoter regulatory elements in transgenic plants under drought, low-temperature, high-salt, and ABA stresses was analyzed. The results showed that the PsDREB2 gene promoter was expressed in the roots, stems, leaves, flowers, and silique pods but not in the seeds of transgenic Arabidopsis. Furthermore, the promoter was induced by drought, low temperature, high salt, and ABA. Hence, the PsDREB2 promoter is tissue- and stress-specific and can be used in the genetic engineering of novel peony cultivars in the future.

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“…Meanwhile, the GBSSI promoter showed very low expression in the first three organs, but very high expression in the latter one. These findings indicated that the cassava GBSSI promoter is the best candidate to achieve tuberous root-specific expression in cassava (Koehorst-van Putten et al, 2012a). Recent research conducted by team of Chinese Academy of Sciences (CAS) successfully produced high amylose transgenic cassava by down regulating BE genes, which are responsible for amylopectin biosynthesis (Zhou et al, 2020).…”

Section: Introductionmentioning

confidence: 96%

Growth and yield performance of four generations of high amylose transgenic Adira 4 cassava

Sudarmonowati¹,

Fitriani²

2021

ipas

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Attempt to improve cassava starch quality have been conducted by producing the first high amylose transgenic Adira 4 Indonesian cassava. This atudy aimed to evaluate agronomic performances of transgenic plants, assessment of four generations in specific containment screenhouse and limited field trial were conducted with improved method. The survival and growth rate of cuttings was enhanced by applying growth regulator such as Atonik and improving planting procedures. The growth and yield performances were measured by comparing related variables across four generations of selected lines. Dipping in 1 mL.L-1 Atonik solution with shading in the early planting were only required when the plant growth was poor and not favourable soil and weather. The result showed that survival of third and fourth generations of Lines A55-5 and A55-15 could reach 100 % when it was treated with Atonik. According to 14 lines assessed, Line A55-5 was one of the promising ones due to its growth, multiplication performance and almost double amylose content compared to wild type. Meanwhile, Line A55-12 showed the highest yield. The finding will benefit functional food industries when appropriate gene construct and approaches were applied for better growth and yield results.

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Cloning and characterization of a tuberous root-specific promoter from cassava (Manihot esculenta Crantz)

Cited by 17 publications

References 30 publications

Isolation and Characterization of Three Cassava Elongation Factor 1 Alpha (MeEF1A) Promoters

Isolation and Characterization of Three Cassava Elongation Factor 1 Alpha (MeEF1A) Promoters

Identification and characterization of PsDREB2 promoter involved in tissue-specific expression and abiotic stress response from Paeonia suffruticosa

Growth and yield performance of four generations of high amylose transgenic Adira 4 cassava

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