Cloning and characterization of a new exo-cellulase gene,<i>cel3</i>, in<i>Irpex lacteus</i>

Hamada, Naoko; Fuse, Naoki; Shimosaka, Makoto; Kodaira, Ritsuko; Amano, Yoshihiko; Kanda, Takahisa; Okazaki, Mitsuo

doi:10.1111/j.1574-6968.1999.tb13473.x

Cited by 19 publications

(7 citation statements)

References 18 publications

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“…The conversion of cellulose into simple forms of carbon may be useful for the production of bioenergy (Bisaria and Ghose, 1981; Schallmey et al , 2004). In general, the degradation of cellulose occurs by the induction of three types of enzymes: endo-1,4-β-D-glucanase (endocellulase), exo-1,4-β-D-glucanase (exocellulase) and β-glucosidase (cellubiose hydrolase) (Eveleigh, 1981; Hamada et al , 1999). Enzymes called endoglucanases or endocellulases represent a group of cellulases active in the cleavage of random regions within cellulose, producing oligosaccha-rides with variable sizes.…”

Section: Introductionmentioning

confidence: 99%

Endo-and exoglucanase activities in bacteria from mangrove sediment

Júnior¹,

Dias²,

Fasanella³

et al. 2013

Braz. J. Microbiol.

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The mangrove ecosystem is an unexplored source for biotechnological applications. In this unique environment, endemic bacteria have the ability to thrive in the harsh environmental conditions (salinity and anaerobiosis), and act in the degradation of organic matter, promoting nutrient cycles. Thus, this study aimed to assess the cellulolytic activities of bacterial groups present in the sediment from a mangrove located in Ilha do Cardoso (SP, Brazil). To optimize the isolation of cellulolytic bacteria, enrichments in two types of culture media (tryptone broth and minimum salt medium), both supplemented with 5% NaCl and 1% of cellulose, were performed. Tests conducted with the obtained colonies showed a higher occurrence of endoglycolytic activity (33 isolates) than exoglycolytic (19 isolates), and the degradation activity was shown to be modulated by the presence of NaCl. The isolated bacteria were clustered by BOX-PCR and further classified on the basis of partial 16S rRNA sequences as Alphaproteobacteria, Gammaproteobacteria, Actinobacteria, Firmicutes or Bacteroidetes. Therefore, this study highlights the importance of studies focusing on the endemic species found in mangroves to exploit them as novel biotechnological tools for the degradation of cellulose.

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Section: Introductionmentioning

confidence: 99%

Endo-and exoglucanase activities in bacteria from mangrove sediment

Júnior¹,

Dias²,

Fasanella³

et al. 2013

Braz. J. Microbiol.

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“…and homologous with P chrysosporium and A bisporus keep similarity of 74% (Chow et al, 1994). Hamada et al (1999) manifested 79% similarity of amino acid catalytic domain comparison cel3 gene with cel2 gene of Irpex lacteus. Truncated cel3 gene in E. coli having only C-terminal region of endoglucanase is able to retain catalytic activity of carboxymethyl cellulose as amino acid sequence are conserved in C-terminal domain of cel3 gene (Jiang et al, 2010).…”

Section: Structure Of Cel3mentioning

confidence: 92%

“…Yagüe et al (1996) also isolated and characterized cellulase gene cel1 and cel2 gene of Irpex lacteus to study regulation of gene sequence where it was found to encode exo-type cellulase, Ex1 and Ex-2. Hamada et al (1999) concluded cellulase genes of Irpex lacteus namely, cel1, cel2 and cel3 were highly similar in their tripartite structure but Hamada et al (1999) mentioned that the cel3 fragment didn't hybridize with cel1 and cel2 whereas Northern hybridization revealed no difference between transcription of cel1, cel2 and cel3 genes in cell of Irpex lacteus. Such gene can be utilized by microorganisms to break primary structure of plant cell wall (Saha et al, 2006).…”

Section: Cel3 Gene In Microorganismsmentioning

confidence: 99%

“…Chow et al (1994) reported cellulase gene cel1 and cel3 cab expression can be controlled at transcriptional level by crystalline cellulose and (Ptashne, 1988) suggested that the promoter region of both genes might contain cellulose responsive elements easily recognized by activator. Phylogeny analysis of glycosyl hydrolase enzymes encoded by namely cel1, cel2 and cel3 have evolved independently and clearly classifiable into two groups as exo-type and endo-type cellulase having cel3 alignment better with exo-type cellulase (Hamada et al, 1999).…”

Section: Cel3 Gene Homologousmentioning

confidence: 99%

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Isolation of Glucose Fermenting Nitrogen Fixing Gene (nifH) Containing Endophytic Bacteria from Seed and Roots of Wheat (Triticum aestivation l.)

Devkota,

Puri,

Yadav

et al. 2023

Preprint

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The present study was conducted to study the nifH gene containing endophytic bacterial frequency in wheat seed and roots obtained from the soil of Chitwan and Kaski. One hundred and four isolates were studied for the presence of the nifH gene. There was a diversity in isolate characters obtained from root sample Root (R), direct seed sample (ds), and plant sample (P). None of the isolates from any sources showed indole-producing ability. About 18 isolates (15% of the total) contained the nifH gene through amplification of the gene by universal primers PolF and PolR. About 6 isolates from seed sample ds and 12 isolates from root sample R contained nifH gene. None of the isolates from root sample P manifested the presence of the nifH gene. Among 18 nifH-containing isolates, only 6 isolates manifested presence of cel3 gene of 400 bp, whereas, 11 isolates showed cel3 gene of 200 bp. All nifH gene containing isolates were confirmed to be bacteria by PCR amplification of 16s rRNA gene by universal primer 27F and 1492R and visualization of agarose gel matrix with bp range of approximately 530 under UV ray. Further research scope exists to use these microbes as a bio fertilizer in plant growth promotion studies by inoculation.

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“…The white rot fungus basidomycete Irpex lacteus grows on fallen wood and decomposes it by secreting multiple enzymes including cellulases, hemicellulases and lignin-degrading enzymes with its strain MC-2 being used as a producer of a commercial enzyme preparation called Driselase marketed by Kyowa Hakko, Co., Ltd. [75]. Hamada et al [114,115] 2005) also isolated a cellulase gene cen1 encoding for a GH5 endoglucanase I termed En-1 (a molecular weight of about 52 kDa) in the basidomycete Irpex lacteus with relatively low specific activities (7.76-25.9 µmol D-glucose/mg protein.min) determined with the soluble substrate CMC [116]. While the basidomycete Irpex lacteus GH5 endoglucanase-I En-1 activity in hydrolyzing Avicel was not reported in the study, the researchers did show that En-1 in combination with the exocellulase Ex-1 had dramatic synergistic effects in enhancing the hydrolysis of crystalline cellulose substrates of Avicel and PASC [116].…”

Section: Properties Of Fungal Biomass Degradation Hemi-cellulases and Cellulasesmentioning

confidence: 99%

Metagenomic Discovery and Characterization of Multi-Functional and Monomodular Processive Endoglucanases as Biocatalysts

et al. 2021

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Biomass includes cellulose, hemicelluloses, pectin and lignin; constitutes the components of dietary fibre of plant and alge origins in animals and humans; and can potentially provide inexhaustible basic monomer compounds for developing sustainable biofuels and biomaterials for the world. Development of efficacious cellulases is the key to unlock the biomass polymer and unleash its potential applications in society. Upon reviewing the current literature of cellulase research, two characterized and/or engineered glycosyl hydrolase family-5 (GH5) cellulases have displayed unique properties of processive endoglucanases, including GH5-tCel5A1 that was engineered and was originally identified via targeted genome sequencing of the extremely thermophilic Thermotoga maritima and GH5-p4818Cel5_2A that was screened out of the porcine hindgut microbial metagenomic expression library. Both GH5-tCel5A1 and GH5-p4818_2A have been characterized as having small molecular weights with an estimated spherical diameter at or < 4.6 nm; being monomodular without a required carbohydrate-binding domain; and acting as processive β-1,4-endoglucanases. These two unique GH5-tCel5A1 and GH5-p4818_2A processive endocellulases are active in hydrolyzing natural crystalline and pre-treated cellulosic substrates and have multi-functionality towards several hemicelluloses including β-glucans, xylan, xylogulcans, mannans, galactomannans and glucomannans. Therefore, these two multifunctional and monomodular GH5-tCel5A1 and GH5-p4818_2A endocellulases already have promising structural and functional properties for further optimization and industrial applications.

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Cloning and characterization of a new exo-cellulase gene,cel3, inIrpex lacteus

Cited by 19 publications

References 18 publications

Endo-and exoglucanase activities in bacteria from mangrove sediment

Endo-and exoglucanase activities in bacteria from mangrove sediment

Isolation of Glucose Fermenting Nitrogen Fixing Gene (nifH) Containing Endophytic Bacteria from Seed and Roots of Wheat (Triticum aestivation l.)

Metagenomic Discovery and Characterization of Multi-Functional and Monomodular Processive Endoglucanases as Biocatalysts

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