Cloning and Characterization of a Candidate Gene from the Medicinal Plant Catharanthus roseus Through Transient Expression in Mesophyll Protoplasts

Duarte, Patrícia; Ribeiro, Diana; Henriques, Gisela; Hilliou, Frédérique; Rocha, Ana Sofia; Lima, Francisco Esmaile de Sales; Amorim, Isabel R.; Sottomayor, Mariana

doi:10.5772/23323

Molecular Cloning - Selected Applications in Medicine and Biology 2011

DOI: 10.5772/23323

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Cloning and Characterization of a Candidate Gene from the Medicinal Plant Catharanthus roseus Through Transient Expression in Mesophyll Protoplasts

Patrícia Duarte¹,

Diana Ribeiro²,

Gisela Henriques³

et al.

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2018

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Vacuole-Targeted Proteins: Ins and Outs of Subcellular Localization Studies

Carqueijeiro¹,

Sepúlveda²,

Mosquera³

et al. 2018

Plant Vacuolar Trafficking

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Accurate and efficient demonstrations of protein localizations to the vacuole or tonoplast remain strict prerequisites to decipher the role of vacuoles in the whole plant cell biology and notably in defence processes. In this chapter, we describe a reliable procedure of protein subcellular localization study through transient transformations of Catharanthus roseus or onion cells and expression of fusions with fluorescent proteins allowing minimizing artefacts of targeting.

show abstract

Vacuole-Targeted Proteins: Ins and Outs of Subcellular Localization Studies

Carqueijeiro¹,

Sepúlveda²,

Mosquera³

et al. 2018

Plant Vacuolar Trafficking

View full text Add to dashboard Cite

show abstract

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Cloning and Characterization of a Candidate Gene from the Medicinal Plant Catharanthus roseus Through Transient Expression in Mesophyll Protoplasts

Cited by 1 publication

References 29 publications

Vacuole-Targeted Proteins: Ins and Outs of Subcellular Localization Studies

Vacuole-Targeted Proteins: Ins and Outs of Subcellular Localization Studies

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