Cloning and characterization of a novel endothelin receptor subtype in the avian class

Lecoin, Laure; Sakurai, Takeshi; Ngo, Minh Triet; Abe, Yoichiro; Yanagisawa, Masashi; Douarin, Nicole M. Le

doi:10.1073/pnas.95.6.3024

Cited by 97 publications

(82 citation statements)

References 44 publications

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“…A paralogue of EDNRB (designated EDNRB2), found to be specific to the melanocytic lineage, was cloned in quail by Lecoin et al (1998). A mutant in quail, which had an amino acid substitution and reduced gene expression in the ENDRB2 gene, displayed white plumage with wild-type colored spots (Miwa et al 2007).…”

Section: Discussionmentioning

confidence: 99%

Gene Duplication of endothelin 3 Is Closely Correlated with the Hyperpigmentation of the Internal Organs (Fibromelanosis) in Silky Chickens

et al. 2012

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During early development in vertebrates, pluripotent cells are generated from the neural crest and migrate according to their presumptive fate. In birds and mammals, one of the progeny cells, melanoblasts, generally migrate through a dorsolateral route of the trunk region and differentiate to melanocytes. However, Silky is an exceptional chicken in which numerous melanoblasts travel via a ventral pathway and disperse into internal organs. Finally, these ectopic melanocytes induce heavy dermal and visceral melanization known as Fibromelanosis (Fm). To identify the genetic basis of this phenotype, we confirmed the mode of inheritance of Fm as autosomal dominant and then performed linkage analysis with microsatellite markers and sequence-tagged site markers. Using 85 backcross progeny from crossing Black Minorca chickens (BM-C) with F 1 individuals between White Silky (WS) and BM-C Fm was located on 10.2-11.7 Mb of chicken chromosome 20. In addition, we noticed a DNA marker that all Silky chickens and the F 1 individuals showed heterozygous genotyping patterns, suggesting gene duplication in the Fm region. By quantitative real-time PCR assay, Silky line-specific gene duplication was detected as an 130-kb interval. It contained five genes including endothelin 3 (EDN3), which encoded a potent mitogen for melanoblasts/melanocytes. EDN3 with another three of these duplicated genes in Silky chickens expressed almost twofold of those in BM-C. Present results strongly suggest that the increase of the expression levels resulting from the gene duplication in the Fm region is the trigger of hypermelanization in internal organs of Silky chickens.

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Section: Discussionmentioning

confidence: 99%

Gene Duplication of endothelin 3 Is Closely Correlated with the Hyperpigmentation of the Internal Organs (Fibromelanosis) in Silky Chickens

et al. 2012

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“…Figure 4a illustrates the RT ± PCR analysis that we performed on RNA from tsMyb/EGFR cells grown at both 378C and 428C. This analysis detected expression of tyrosinase (1006) which is required for the synthesis of melanin, the slug zinc ®nger protein which is expressed on migrating neural crest cells (Nieto et al, 1994), the bHLH protein Mitf which is essential for melanocyte di erentiation (Opdecamp et al, 1997), the endothelin receptor (EDNRB2, Lecoin et al, 1998), melanotransferrin (EOS47, McNagny et al, 1996, the chicken homologue of the melanoma associated antigen MUC18 (HEMCAM, Vainio et al, 1996) and c-Myb. We also consistently detected the expression of HEMCAM using the antibody JS3 (not shown).…”

Section: The Transformed and DI Erentiating Cells Express Melanocyte mentioning

confidence: 99%

v-Myb can transform and regulate the differentiation of melanocyte precursors

Bell

Frampton

1999

Oncogene

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The activity of the c-Myb transcription factor is essential for the development of de®nitive multi-and uni-lineage progenitors of the haemopoietic system. Re¯ecting this requirement, c-Myb has been oncogenically activated by transduction in the E26 avian retrovirus which elicits an acute leukaemia by transforming haemopoietic progenitors. Here, we report the novel ®nding that Myb in cooperation with EGF receptor signalling can be used to generate clonally expanded populations of transformed cells which have the phenotype of melanocyte precursors. Through the use of a conditional temperature sensitive mutant of Myb, we show that in the transformed cells Myb regulates commitment to melanocyte di erentiation and possibly proliferation. These results add to our understanding of the roles of c-Myb beyond the haemopoietic system and to our knowledge and means of investigating the importance of transcription factors in the melanocyte linage.

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“…44,45 In our laboratory, we could demonstrate the strong implication of the vasoactive peptide endothelin-3 in the development of the melanocytic and glial lineages both in culture and in vivo. 18,35,[46][47][48][49] …”

Section: Cytokine-like Factors In Neuropoiesismentioning

confidence: 99%

The stem cells of the neural crest

Douarin

Calloni²,

Dupin³

2008

Cell Cycle

Self Cite

126

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In the vertebrate embryo, the neurectodermal neural crest cells (NCC) have remarkably broad potencies, giving rise, after a migratory phase, to neurons and glial cells in the peripheral nervous system, and to skin melanocytes, being all designated here as "neural" derivatives. NC-derived cells also include non-neural, "mesenchymal" cell types like chondrocytes and bone cells, myofibroblasts and adipocytes, which largely contribute to the head structures in amniotes. Similar to the blood cell system, the NC is therefore a valuable model to investigate the mechanisms of cell lineage diversification in vertebrates. Whether NCC are endowed with multiple differentiation potentials or if, conversely, they are a mosaic of different committed cells is an important ongoing issue to understand the ontogeny of NC derivatives in normal development and pathological conditions. Here we focus on recent findings that established the presence in the early migratory NC of the avian embryo, of a multipotent progenitor endowed with both mesenchymal and neural differentiation capacities. This "mesenchymal-neural" clonogenic cell lies upstream of all the other NC progenitors known so far and shows increased frequency when single cell cultures are treated with the Sonic Hedgehog signaling molecule. These findings are discussed in the context of the broad potentials of NC stem cells recently evidenced in certain adult mammalian tissues.

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Cloning and characterization of a novel endothelin receptor subtype in the avian class

Cited by 97 publications

References 44 publications

Gene Duplication of endothelin 3 Is Closely Correlated with the Hyperpigmentation of the Internal Organs (Fibromelanosis) in Silky Chickens

Gene Duplication of endothelin 3 Is Closely Correlated with the Hyperpigmentation of the Internal Organs (Fibromelanosis) in Silky Chickens

v-Myb can transform and regulate the differentiation of melanocyte precursors

The stem cells of the neural crest

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