Cloning and characterization of a novel UDP-glycosyltransferase gene induced by DON from wheat

Abstract

Fusarium head blight, caused primarily by Fusarium graminearum, is a destructive disease of wheat throughout the world. However, the mechanisms of host resistance to FHB are still largely unclear. Deoxynivalenol (DON) produced by F. graminearum which enhances the pathogen to spread could be converted into inactive form D3G by UDP-glycosyltransferases (UGTs). A DON responsive UGT gene, designated as TaUGT4, was first cloned from wheat in this study. The putative open reading frame (ORF) of TaUGT4 was 1386 bp, encoding 461 amino acids protein. TaUGT4 was placed on chromosome 2D using a set of nulli-tetrasomic lines of wheat cultivar Chinese Spring (CS). When fused with eGFP at C terminal, TaUGT4 was shown to localize in cytoplasm of the transformed tobacco cells. The transcriptional analysis revealed that TaUGT4 was strongly induced by F. graminearum or DON in both of FHB-resistant cultivar Sumai 3 and susceptible cultivar Kenong 199, especially in Sumai 3 under DON treatment. Similar increase of TaUGT4 expression was observed in Sumai 3 and Kenong 199 in response to salicylic acid (SA) treatment. But interestingly, the transcripts level of TaUGT4 in Sumai 3 showed significantly higher than that in Kenong 199 after treated with methyl jasmonate (MeJA). According to the expression patterns, TaUGT4 might lead to different effects between FHB-resistant genotype and susceptible genotype in the process against F. graminearum inoculation. It had also been discussed in this paper that JA signaling pathway might play a significant role in the resistance against F. graminearum compared to SA signaling pathway.

TaUGT6, a Novel UDP-Glycosyltransferase Gene Enhances the Resistance to FHB and DON Accumulation in Wheat

“…Fluorescence observation revealed that TaUGT6 was distributed throughout cells including in cell membrane and nuclei, similar to that in pA7-eGFP (Figure 3). Its subcellular localization was similar to that reported for TaUGT3 but different from those reported for TaUGT4 and TaUGT5 (Ma et al, 2010(Ma et al, , 2015Zhao et al, 2018).…”

“…In wheat, few UGT genes have been identified and proven to be functional in FHB resistance. TaUGT1 was repressed, and TaUGT2 and TaUGT4 with high similarity to HvUGT13248 were induced after F. graminearum inoculation, but no further functional analysis was conducted (Lin et al, 2008;Ma et al, 2015). Thus far, only TaUGT3, TaUGT5, and Traes_2BS_14CA35D5D (orthologous to Bradi5g03300) in wheat have been transformed into plants to prove their role in FHB resistance and DON tolerance (Gatti et al, 2018;Xing et al, 2018;Zhao et al, 2018).…”

“…TaUGT6 and several other UGT proteins (TaUGT1, TaUGT2, TaUGT3, TaUGT4, TaUGT5, HvUGT13248, Bradi5g03300, and DOGT1) that had been previously reported to contribute to FHB resistance were selected for alignment by DNAMAN using the pairwise method ( Poppenberger et al, 2003 ; Lin et al, 2008 ; Ma et al, 2015 ; Pasquet et al, 2016 ; Li et al, 2017 ; Xing et al, 2018 ; Zhao et al, 2018 ). BLAST analysis of the full-length amino acid sequences of TaUGT6 was performed to identify close homologs of TaUGT6 in the Phytozome database 1 .…”

“…TaUGT1 , TaUGT2 , and TaUGT4 were only analyzed at the transcriptional level following F . graminearum infection and were not further characterized ( Lin et al, 2008 ; Ma et al, 2015 ). TaUGT12887/TraesCS5B02G148300 conferred increased DON resistance when expressed in yeast ( Schweiger et al, 2013b ).…”

Genome-wide analysis of family-1 UDP glycosyltransferases (UGT) and identification of UGT genes for FHB resistance in wheat (Triticum aestivum L.)

“…Recently the HvUGT-10 W1 gene isolated from an FHB resistant barley variety (10 W1) also conferred FHB tolerance [ 34 ]. In wheat only a few candidate UGT genes such as TaUGTB2 , TaUGT1 , TaUGT2 , TaUGT3 , TaUGT4 , and TaUGT12887 have been identified and the later 4 genes have been shown to be closely related to FHB resistance; however, a large scale systematic investigation of UGTs in the wheat genome is still lacking [ 35 – 39 ].…”