Cloning and characterization of a pyrethroid pesticide decomposing esterase gene, Est3385, from Rhodopseudomonas palustris PSB-S

Abstract

Full length open reading frame of pyrethroid detoxification gene, Est3385, contains 963 nucleotides. This gene was identified and cloned based on the genome sequence of Rhodopseudomonas palustris PSB-S available at the GneBank. The predicted amino acid sequence of Est3385 shared moderate identities (30–46%) with the known homologous esterases. Phylogenetic analysis revealed that Est3385 was a member in the esterase family I. Recombinant Est3385 was heterologous expressed in E. coli, purified and characterized for its substrate specificity, kinetics and stability under various conditions. The optimal temperature and pH for Est3385 were 35 °C and 6.0, respectively. This enzyme could detoxify various pyrethroid pesticides and degrade the optimal substrate fenpropathrin with a Km and Vmax value of 0.734 ± 0.013 mmol·l−1 and 0.918 ± 0.025 U·µg−1, respectively. No cofactor was found to affect Est3385 activity but substantial reduction of enzymatic activity was observed when metal ions were applied. Taken together, a new pyrethroid degradation esterase was identified and characterized. Modification of Est3385 with protein engineering toolsets should enhance its potential for field application to reduce the pesticide residue from agroecosystems.

Insight Into Microbial Applications for the Biodegradation of Pyrethroid Insecticides

“…Cloning was carried out from metagenomic library of soil samples and sequence analysis revealed that 819 bp pye3 gene codes for 273 amino acid protein. Enzyme was further characterized on the basis of enzyme kinetics ( K m and K cat activity) (Li et al, 2008; Luo et al, 2018).…”

Phytoremediation and Bioremediation of Pesticide-Contaminated Soil

“…Genetic engineering research has been conducted to develop microorganisms that are effective for the biodegradation of pesticides and pesticide residues [47,60,179,205,287]. Hussain and coworkers have reviewed research progress on this topic [111].…”

Whole Genome Sequencing and Analysis of Fenvalerate Degrading Bacteria Citrobacter freundii CD-9

“…The carboxylesterase EstSt7 from Sulfolobus tokadaii, has been shown to hydrolyze a variety of synthetic pyrethroids, including type I and type II pyrethroid pesticides (Wei et al 2013). The pyrethroid-degrading gene coding for Est3385 from R. palustris PSB-S was also cloned and expressed using previously published genes (Luo et al 2018). The construction of pesticide-degrading engineered bacteria with broad-spectrum and e cient degradation ability by mining more functional genes has attracted much attention, and will open new avenues for the remediation of environmental pollution.…”