Cloning and Characterization of a Rice Field Eel vasa-Like Gene cDNA and Its Expression in Gonads During Natural Sex Transformation

Ding, Ye; Lv, Daoyuan; Song, Ping; Peng, Maoyu; Chen, Yungui; Guo, Ming; Yang, Qiwen; Hu, Yinchang

doi:10.1007/s10528-006-9066-6

Cited by 30 publications

(17 citation statements)

References 43 publications

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“…The tissue expression profile of the Vasa gene in adult toadfish was performed by qPCR without differentiating between the transcripts, because most of these transcripts showed minimal molecular differences. Vasa expression was principally restricted to the gonads, in agreement with the role of Vasa as a translational regulator in germinal line development (Braat et al 1999a), and as in other adult fish species (Xu et al 2005, Ye et al 2007, Nagasawa et al 2009, Li et al 2010, Blázquez et al 2011, Cao et al 2012, Lin et al 2012, Presslauer et al 2012, Xiao et al 2013. However, the Vasa gene could also be in volved in the regulation of translation of certain mRNA, which is essential for the specification of somatic cells where Vasa protein is present (Ikenishi & Tanaka 2000).…”

Section: Discussionsupporting

confidence: 71%

“…In toadfish testes, the expression pattern of Vasa gene showing positive signals in spermatogonia, spermatocytes and spermatids was similar to the cellular distribution pattern reported in Senegalese sole (Pacchiarini et al 2013b). However, in most fish species, Vasa gene expression was observed only in spermatogonia and spermatocytes (Kobayashi et al 2000, Xu et al 2005, Ye et al 2007, Cao et al 2012, Lin et al 2012, Xiao et al 2013, Pacchiarini et al 2013a, whereas in bluefin tuna Vasa mRNA was restricted to spermatogonia only (Nagasawa et al 2009).…”

Section: Discussionmentioning

confidence: 99%

“…In many fish species, Vasa mRNA ISH signals are most intense in oogonia and early vitellogenic oocytes, decreasing in more advanced vitellogenenic stages (Braat et al 1999b, Xu et al 2005, Nagasawa et al 2009, Lin et al 2012, Ye at al. 2007, Pacchiarini et al 2013a.…”

Section: Discussionmentioning

confidence: 99%

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Cloning and characterization of Vasa gene expression pattern in adults of the Lusitanian toadfish Halobatrachus didactylus

Úbeda-Manzanaro

Rebordinos²,

Sarasquete³

2014

Aquat. Biol.

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The Vasa gene is essential for germ cell development in eukaryotes. It encodes a RNA helicase, a member of the DEAD box protein family. Using the RACE method, we cloned the Vasa cDNA of the Lusitanian toadfish Halobatrachus didactylus, and analyzed quantitative and qualitative Vasa expression and its protein immunolocalization. We reported a main product of about 2.4 kb which encodes a protein of 615 amino acids, but other minority Vasa products were also identified by RACE-PCR. This gene is predominantly expressed in the ovaries and testes, although some relatively low extragonadal expression levels have also been identified. In situ hybridization and immunolocalization analysis during gametogenesis in the testes showed that toadfish Vasa mRNA was detected in spermatogonia, spermatocytes and spermatids. However, in the ovaries, Vasa mRNA was detected in early vitellogenic oocytes and in more advanced vitellogenic stages, showing a very weak signal in oogonia, whereas the Vasa protein was evidenced in the cytoplasm of oogonia and previtellogenic oocytes, becoming weaker as the vitellogenic and maturation processes progress. These results suggest that toadfish Vasa homologues can play an important role in gametogenesis and germ cell development, but it could also be functionally implicated in other processes that are not as well known.

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Section: Discussionsupporting

confidence: 71%

Section: Discussionmentioning

confidence: 99%

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Cloning and characterization of Vasa gene expression pattern in adults of the Lusitanian toadfish Halobatrachus didactylus

Úbeda-Manzanaro

Rebordinos²,

Sarasquete³

2014

Aquat. Biol.

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“…This is an intriguing phenomenon and has been under extensive investigation; however, the mechanism of natural sex change in the ricefield eel remains unclear (Ye et al 2007). The purpose of this study was to elucidate the involvement of cyp19 gene in the process of sex change in the ricefield eel.…”

Section: Introductionmentioning

confidence: 99%

Two cytochrome P450 aromatase genes in the hermaphrodite ricefield eel Monopterus albus: mRNA expression during ovarian development and sex change

Zhang

Yang

et al. 2008

Journal of Endocrinology

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Previously, the ricefield eel (Monopterus albus) was speculated to have only one cytochrome p450 aromatase gene. In this study, however, the cDNAs encoding two distinct cytochrome p450 aromatases, cyp19a1a and cyp19a1b, were isolated. The genomic organizations of both cyp19 genes were conserved when compared with other teleosts. Northern blot detected an abundant expression of cyp19a1a in the ovary, and cyp19a1b in the hypothalamus. RT-PCR coupled with Southern blot showed that cyp19a1a was expressed predominantly in the gonads of both sexes, with higher levels in the ovary than testis, while cyp19a1b was expressed in all the tissues examined in the male, but only in the brain and pituitary in the female. The levels of cyp19a1a mRNA in the ovary were increased significantly during vitellogenesis, but decreased significantly at mature stage. The levels of cyp19a1b mRNA in the brain and pituitary did not vary significantly during vitellogenesis. As ovarian development shifted from vitellogenesis to maturation, the levels of cyp19a1b mRNA was decreased significantly in the brain, but increased significantly in the pituitary. During natural sex change from female to male, the levels of cyp19a1a mRNA in the gonad were significantly decreased. The levels of cyp19a1b mRNA in the hypothalamus were significantly increased at the early intersexual phase, whereas the expression levels in the pituitary were significantly decreased at the intersexual phases. Taken together, these results showed a novel sexual dimorphism of cyp19a1b mRNA tissue distribution, and both CYP19 genes were associated with the ovarian development and natural sex change of the ricefield eel.

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“…Therefore, this species is a good model for comparative genomic studies on vertebrate sexual development . Studies on sex determination/differentiation in the rice field eel have mainly focused on a few individual genes, such as the sox family (SRY-related box gene) [Zhou et al, 2002], dmrt family (doublesex and mab3-related transcription factor) [Jang et al, 2006], vasa (vasa-related gene) [Ye et al, 2007], cyp19a (cytochrome P450 aromatase, family 19, subfamily A) [Yu et al, 2008], and jnk1 (c-Jun N-terminal kinases) [Xiao et al, 2010]. Previous studies on the genetic control of sexual differentiation in the rice field eel have rarely involved investigations on the roles of miRNA expression patterns in this process.…”

mentioning

confidence: 99%

Characterization and Differential Expression Patterns of Conserved microRNAs and mRNAs in Three Genders of the Rice Field Eel <b><i>(Monopterus albus)</i></b>

Gao

Guo

et al. 2014

Sex Dev

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MicroRNAs (miRNAs) are endogenous small RNAs that can regulate target mRNAs by binding to their sequences in the 3′ untranslated region. The expression of miRNAs and their biogenetic pathway are involved in sexual differentiation and in the regulation of the development of germ cells and gonadal somatic cells. The rice field eel (Monopterus albus) undergoes a natural sexual transformation from female to male via an intersex stage during its life cycle. To investigate the molecular mechanisms of this sexual transformation, miRNAs present in the different sexual stages of the rice field eel were identified by high-throughput sequencing technology. A significantly differential expression among the 3 genders (p < 0.001) was observed for 48 unique miRNAs and 3 miRNAs*. Only 9 unique miRNAs showed a more than 8-fold change in their expression among the 3 genders, including mal-miR-430a and mal-miR-430c which were higher in females than in males. However, mal-miR-430b was only detected in males. Several potential miRNA target genes (cyp19a, cyp19b, nr5a1b, foxl2 amh, and vasa) were also investigated. Real-time RT-PCR demonstrated highly specific expression patterns of these genes in the 3 genders of the rice field eel. Many of these genes are targets of mal-miR-430b according to the TargetScan and miRTarBase. These results suggest that the miR-430 family may be involved in the sexual transformation of the rice field eel.

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Cloning and Characterization of a Rice Field Eel vasa-Like Gene cDNA and Its Expression in Gonads During Natural Sex Transformation

Cited by 30 publications

References 43 publications

Cloning and characterization of Vasa gene expression pattern in adults of the Lusitanian toadfish Halobatrachus didactylus

Cloning and characterization of Vasa gene expression pattern in adults of the Lusitanian toadfish Halobatrachus didactylus

Two cytochrome P450 aromatase genes in the hermaphrodite ricefield eel Monopterus albus: mRNA expression during ovarian development and sex change

Characterization and Differential Expression Patterns of Conserved microRNAs and mRNAs in Three Genders of the Rice Field Eel <b><i>(Monopterus albus)</i></b>

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