Cloning and Characterization of a Probenazole-Inducible Gene for an Intracellular Pathogenesis-Related Protein in Rice

Abstract

Probenazole (3-allyloxy-1,2-benzisothiazole-1,1-dioxide) induces disease resistance in rice against rice blast fungus. To investigate the molecular mechanism of probenazole-induced resistance, we isolated and characterized a cDNA clone of a probenazole-inducible gene in rice, which encoded a protein designated PBZ1. Sequence analysis revealed that significant homology at the amino acid level exists between the predicted PBZ1 protein and intracellular pathogenesis-related (IPR) proteins. Accumulation of PBZ1 mRNA was not induced by wounding, but markedly induced by inoculation with rice blast fungus. In addition, it was induced sooner by inoculation with an incompatible race than that with a compatible race. On the other hand, when the accumulation of the PBZ1 mRNA was examined after treatment with probenazole-related compounds, it was not fully correlated with anti-rice blast activity. However, it was induced after treatment with N-cyano-methyl-2-chloro-isonicotinamide (NCI), which belongs to another group of compounds known to induce disease resistance. Thus, although the accumulation of the PBZ1 mRNA was not fully correlated with anti-rice blast activity, our findings suggest that the PBZ1 gene has an important function during the disease resistance response in rice.

Sekiguchi Lesion Gene Encodes a Cytochrome P450 Monooxygenase That Catalyzes Conversion of Tryptamine to Serotonin in Rice

“…C, HPLC analysis of reaction products after the microsomal SL proteins were assayed for enzyme activity using tryptamine as a substrate. from fungal pathogens (25). 6 Expression of these defense genes was clearly induced at 12 h after serotonin treatment, but no induction was found with tryptamine ( Fig.…”

The rice Rim2 transcript accumulates in response to Magnaporthe grisea and its predicted protein product shares similarity with TNP2-like proteins encoded by CACTA transposons

“…These genes include those for 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) (Nelson et al 1994), lipoxygenase (Peng et al 1994), phenylalanine ammonia-lyase (PAL) and chitinase (Xu et al 1996). Transcripts of some other rice genes, such as PBZ1 (Midoh and Iwata 1996), RMa1 and RMa3 , accumulate in response to infection by M. grisea. Likewise, transcription of rice genes for HMGR, chitinase and GDP-dissociation inhibitors (GDIs) is induced by elicitors derived from M. grisea cell wall (Nelson et al 1994;Xu et al 1996;Kim et al 1999).…”

The RNA Hydrolysis and the Cytokinin Binding Activities of PR-10 Proteins Are Differently Performed by Two Isoforms of the Pru p 1 Peach Major Allergen and Are Possibly Functionally Related

“…They were first identified in parsley (Petroselinum crispum; Somssich et al, 1988) and later isolated from several plant species and tissues (Matton and Brisson, 1989;Crowell et al, 1992;Warner et al, 1994;Breda et al, 1996;Midoh and Iwata, 1996;Huang et al, 1997;Lo et al, 1999;Yu et al, 2000;Wu et al, 2003). PR-10 proteins are typically small (from 155-163 residues) cytoplasmic proteins with acidic pI (Liu and Ekramoddoullah, 2006).…”