Cloning and characterisation of tandem-repeat type galectin in rainbow trout (Oncorhynchus mykiss)

Molecular characterization of a tandem-repeat galectin-9 (RuGlec9) from Korean rose bitterling (Rhodeus uyekii)

“…This result is consistent with previous observations showing ubiquitous expression, especially in immune-related tissues, including bone marrow, spleen, thymus, and lymph nodes, in humans and in the hemocytes of pearl oysters [5,18]. The 1.6-kb mRNA of the tandem-repeat galectin from rainbow trout was also widely expressed in the spleen, head, kidney, thymus, peritoneal exudate cells, ovaries, gills, and heart [16]. This is the first time that galectin-9 expression was detected in the testis, although previous studies reported the expression and possible function of galectin-1 and -3 in the testis [23e25].…”

“…Several galectin-9 sequences have been identified from teleost fish, including Atlantic salmon Salmo salar (ACI67584), rainbow trout Oncorhynchus mykiss (ACO08221), catfish Ictalurus punctatus (NP_001187343), and zebrafish Danio rerio (NP_001001817) [16]. More recently, it was shown that tandem-repeat galectin-9 homologs may be involved in innate immunity in S. salar and the pearl oyster Pinctada fucata after infection with infectious pancreatic necrosis virus (IPNV) and Vibrio alginolyticus, respectively [17,18].…”

A tandem-repeat galectin involved in innate immune response of the pearl oyster Pinctada fucata

“…Marine Genomics j o u r n a l h o m e p a g e : w w w. e l s ev i e r. c o m / l o c a t e / m a r g e n tissues (Barondes et al, 1994a,b;Inagawa et al, 2001;Yu et al, 2007;Kim et al, 2008). By binding to a wide array of glycoconjugates, galectins have been implicated in a variety of cellular functions including cell adhesion/ migration, differentiation/proliferation, development/apoptosis, tumor cell metastasis and immunity (Hernandez and Baum, 2002;Huang et al, 2007;Yoshino et al, 2008).…”

“…prototype, chimera type and tandem-repeat type (Barondes et al, 1994a,b;Copper, 2002;Leffler et al, 2004;Yu et al, 2007). The prototypes possess molecular weights of 14-18 kDa with a single galactoside binding domain, and are found in many cells and tissues (Inagawa et al, 2001;Yu et al, 2007;Kim et al, 2008). Gal-3, with an apparent molecular weight between 26 and 30 kDa, is the only chimera type galectin found to date, has a collagen like domain and is abundant in activated macrophages and epithelial cells (Inagawa et al, 2001;Yu et al, 2007).…”

“…The prototypes possess molecular weights of 14-18 kDa with a single galactoside binding domain, and are found in many cells and tissues (Inagawa et al, 2001;Yu et al, 2007;Kim et al, 2008). Gal-3, with an apparent molecular weight between 26 and 30 kDa, is the only chimera type galectin found to date, has a collagen like domain and is abundant in activated macrophages and epithelial cells (Inagawa et al, 2001;Yu et al, 2007). The tandem-repeat types (Gal-4, -6, -8, -9 and -12) possess molecular weights of 33-39 kDa, which have two carbohydrate binding domains joined by a linker peptide and are present in various Marine Genomics 4 (2011) …”