Cloning and characterisation of a zona pellucida 3 cDNA from a marsupial, the brushtail possum Trichosurus vulpecula

McCartney, Carmen A.; Mate, Karen E.

doi:10.1017/s0967199499000337

Cited by 29 publications

(10 citation statements)

References 25 publications

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“…The conservation of the ZP domain in such evolutionary divergent species as teleost fish, amphibians, birds, marsupials (Mate and McCartney, 1998;McCartney and Mate, 1999), and mammals suggests that not only has this protein domain been duplicated in vertebrates but that it has been conserved and used as an egg envelope protein in species that diverged several million years ago.…”

Section: Gene Organizationmentioning

confidence: 99%

Oocyte zona pellucida proteins

2007

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Section: Gene Organizationmentioning

confidence: 99%

Oocyte zona pellucida proteins

2007

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“…The zona pellucida glycoconjugate content of several marsupial species was investigated using differential lectin histochemistry [Chapman et al, 2000;Breed at al., 2002] to show interspecific variation in glycoconjugate content, with mannose-containing glycoconjugates exhibiting the greatest variation. Considerable homology exists between the three marsupial zona proteins, ZP1, 2 and 3, and those in eutherians [Mate and McCartney, 1998;McCartney and Mate, 1999;Haines et al, 1999] suggesting that the carbohydrate component might be significant for cell-zona attachment.…”

Section: Embryonic Coatsmentioning

confidence: 99%

Conceptus Polarity and Cell-Zona Adhesion during Early Cleavage (Fertilized Tubal Egg to 8-Cell Stage) in the Marsupial <i>Sminthopsis macroura</i>

Kress

Selwood

2003

Cells Tissues Organs

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This study outlines the ultrastructural changes that occur in Sminthopsis macroura tubal zygotes to the 8-cell stage in relation to observations of development in vitro, oocyte polarity and cell-zona adhesion. The extremely polarized mature oocytes and zygotes have nuclear material at one pole and accumulated vesicular bodies at the other. The first division is associated with extrusion of vesicular bodies and some cytoplasm as a membrane-bound yolk mass into the perivitelline space. Early cleavage is accompanied by the appearance of an extensive, highly structured extracellular matrix (ECM) comprised of amorphous substance, granules and filaments. At the 2- and 4-cell stage the decrease in density of the ECM in the vicinity of the blastomeres may facilitate cell-zona contact. At the 8-cell stage, discharge of vesicular bodies, which mostly appear to be empty, may contribute to the ECM by increasing the area of plasma membrane for synthesis of a hyaluronan-like ECM. As in other marsupials, the precedence of cell-zona adhesion over cell-cell contacts prevents morula formation. The earliest cell-zona contacts appear when microvilli contact the zona in the uterine zygote 12–16 h after uterine entry and continue at later stages. This early contact is possible because of the absence of a dense subzonal ECM in this species. Between late zygote and late 4-cell stage the cytoplasm also contains, beside a large amount of vesicular bodies, demarcated areas where smooth endoplasmic reticulum encloses mitochondria, vesicles, granular material and fibrillar arrays. The latter develop in the late zygote stage and are found outside demarcated areas as well, often closely surrounding large vesicles, probably helping vesicle extrusion. A putative germ plasm was identified at the 4-cell stage.

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“…A number of recent studies have established that ZPA (= mouse ZP2) [Mate and McCartney, 1998], ZPB (= mouse ZP1) [Haines et al, 1999] and ZPC (= mouse ZP3) [McCartney and Mate, 1999] are present in marsupials. A summary of the sequence identity of the nucleic acids and amino acids of the three zona proteins is shown in table 2.…”

Section: Zona Pellucidamentioning

confidence: 99%

Marsupial Egg and Embryo Coats

Selwood

2000

Cells Tissues Organs

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Egg and embryo coats of marsupials are reviewed. Marsupial eggs are enclosed by a zona pellucida, mucoid coat and an outer shell coat. An extra-cellular matrix coat that lines the zona pellucida also occurs in some species. The zona pellucida consists of three zona proteins (ZPA, ZPB, ZPC) with considerable similarity to those of eutherian mammals. The zona is thought to be the sperm receptor site and, in some species, but not others, is a barrier to polyspermy. Immunostaining, in situ hybridisation, histochemistry and electron microscopy indicate that the zona originates in the oocyte at the onset of oocyte growth. It changes greatly in shape during ovulation and early development and plays an essential role in blastocyst formation. The mucoid coat is an acid glycoprotein that is produced by non-ciliated cells at all levels of the oviduct and has been implicated in providing a barrier to polyspermy, nourishment of the embryo and an osmotically stable environment for the embryo. Its width varies widely in marsupials. The shell coat is secreted by the epithelia of the utero-tubal junction and upper uterus during oestrus and early cleavage. Evidence is provided for a second wave of secretion at bilaminar and trilaminar blastocyst stages. Shell-free embryos do not survive in vitro during bilaminar and early trilaminar blastocyst stages. Hatching from the shell coat occurs between 65 and 85% of the gestation period usually when the embryo is undergoing early somitogenesis. The extra-cellular matrix is secreted by the oocyte or by the cells of early cleavage stages and initially separates cells from the zona pellucida and from each other. It plays an essential role in blastocyst formation. The zona, mucoid and shell coats provide a framework for blastocyst construction and normal embryos do not form in their absence in some species.

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Cloning and characterisation of a zona pellucida 3 cDNA from a marsupial, the brushtail possum Trichosurus vulpecula

Cited by 29 publications

References 25 publications

Oocyte zona pellucida proteins

Oocyte zona pellucida proteins

Conceptus Polarity and Cell-Zona Adhesion during Early Cleavage (Fertilized Tubal Egg to 8-Cell Stage) in the Marsupial <i>Sminthopsis macroura</i>

Marsupial Egg and Embryo Coats

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