Cloning and cellular expression of aquaporin 9 in ovine fetal membranes

Wang, Shengbiao; Chen, Jiexiong; Huang, Bing; Ross, Michael G.

doi:10.1016/j.ajog.2005.01.019

Cited by 24 publications

(8 citation statements)

References 25 publications

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“…Signal intensity for AQP1 was highest in amnion and least in allantois, while AQP3, AQP9, and AQP11 were most expressed in chorion and cotyledon but least in amnion. These observations are consistent with the previous reports (Johnston et al 2000;Wang et al 2005). Endogenous b-actin was used as an internal reference.…”

Section: Target Genesupporting

confidence: 93%

“…AQP3 mRNA was found to localize in trophoblast cells of the chorion and fibroblasts of the amnion, while AQP1 was absent from amniotic epithelial cells but present in vascular endothelial cells of amnion and chorion (Johnston et al 2000). AQP9 was found to express in ovine amniotic epithelium (Wang et al 2005). The presence of AQP8 and AQP11 in amnion of sheep has not been reported.…”

Section: Introductionmentioning

confidence: 88%

“…AQP9 was found to express in ovine amniotic epithelium (Wang et al. ). The presence of AQP8 and AQP11 in amnion of sheep has not been reported.…”

Section: Introductionmentioning

confidence: 99%

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Aquaporins in ovine amnion: responses to altered amniotic fluid volumes and intramembranous absorption rates

Cheung

Anderson

Brace

2016

Physiological Reports

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Aquaporins (AQPs) are transmembrane channel proteins that facilitate rapid water movement across cell membranes. In amniotic membrane, the AQP‐facilitated transfer of water across amnion cells has been proposed as a mechanism for amniotic fluid volume (AFV) regulation. To investigate whether AQPs modulate AFV by altering intramembranous absorption (IMA) rate, we tested the hypothesis that AQP gene expression in the amnion is positively correlated with IMA rate during experimental conditions when IMA rate and AFV are modified over a wide range. The relative abundances of AQP1, AQP3, AQP8, AQP9, and AQP11 mRNA and protein were determined in the amnion of 16 late‐gestation ovine fetuses subjected to 2 days of control conditions, urine drainage, urine replacement, or intraamniotic fluid infusion. AQP mRNA levels were determined by RT‐qPCR and proteins by western immunoblot. Under control conditions, mRNA levels among the five AQPs differed more than 20‐fold. During experimental treatments, mean IMA rate in the experimental groups ranged from 100 ± 120 mL/day to 1370 ± 270 mL/day. The mRNA levels of the five AQPs did not change from control and were not correlated with IMA rates. The protein levels of AQP1 were positively correlated with IMA rates (r 2 = 38%, P = 0.01) while the remaining four AQPs were not. These findings demonstrate that five AQPs are differentially expressed in ovine amnion. Our study supports the hypothesis that AQP1 may play a positive role in regulating the rate of fluid transfer across the amnion, thereby participating in the dynamic regulation of AFV.

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Section: Target Genesupporting

confidence: 93%

Section: Introductionmentioning

confidence: 88%

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Aquaporins in ovine amnion: responses to altered amniotic fluid volumes and intramembranous absorption rates

Cheung

Anderson

Brace

2016

Physiological Reports

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“…Because even the major clades emerged within a short time span, it has proven difficult to root the mammalian tree (Nishihara et al, 2009;Dos et al, 2012). Much evidence favours the configuration in the tree we used for our analysis (Douady et al, 2002), where Afrotheria and Xenarthra are sister taxa and sister to all other mammals (Waters et al, 2007;Meredith et al, 2011;Dos et al, 2012), but there are dissenting views (Beck et al, 2006;McCormack et al, 2012). In respect of the character states found in the eutherian common ancestor, our findings are in general agreement with the analyses of O'Leary et al, although they placed Xenarthra near the root of the tree (O' Leary et al, 2013).…”

Section: The Mammalian Tree Is a Work In Progressmentioning

confidence: 99%

“…Maintenance of constant volumes can be explained only by uptake to the fetal vessels of the amniochorion and chorioallantois, known as the intramembranous pathway (Gilbert, 1999). This may be mediated by aquaporin 9, a water channel that is expressed in amniotic and allantoic epithelia, but not in the chorion or placentomes (Wang et al, 2005).…”

Section: Amniotic and Allantoic Fluidsmentioning

confidence: 99%

Advances in flow cytometry in basic and applied equine andrology

2017

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Molecular phylogenetics has made a substantial contribution to our understanding of the relationships between mammalian orders and has generated trees that can be used to examine the evolution of anatomical and physiological traits. We here summarize findings on fetal membranes and placentation in Ferungulata, a clade comprising carnivores, pangolins, and even-and odd-toed ungulates. Their early ontogeny shows several conserved traits such as superficial attachment of the blastocyst, amnion formation by folding, a large allantoic sac and a temporary yolk sac placenta. In contrast, several characters of the chorioallantoic placenta are derived, including the diffuse and cotyledonary placental types in ungulates and zonary placenta in carnivores, specializations of the interhaemal barrier, the presence of areolae or haemophagous regions and lack of stromal decidual cells. Ungulates produce large amounts of placental proteins including placental lactogens and pregnancy-associated glycoproteins. Evolutionary innovations of the placental system may contribute to the high diversity of lifestyles within Ferungulata and be linked to the evolution of highly precocial offspring in ungulates.

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