Cloning and biochemical characterization of indole-3-acetic acid-amino acid synthetase PsGH3 from pea

Ostrowski, Maciej; Mierek‐Adamska, Agnieszka; Porowińska, Dorota; Goc, Anna; Jakubowska, Anna

doi:10.1016/j.plaphy.2016.05.031

Cited by 9 publications

(5 citation statements)

References 47 publications

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“…The proteins encoded by these genes are 92% identical, and the plasmid-located copy has been expressed heterologously and functionally characterized [ 37 ]. IAAL is postulated to convert free IAA into less active conjugated forms [ 38 ]. Heterologous expression of IAAL in tobacco and potato led to abnormal developmental changes [ 39 ].…”

Section: Resultsmentioning

confidence: 99%

Re-programming of Pseudomonas syringae pv. actinidiae gene expression during early stages of infection of kiwifruit

et al. 2018

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BackgroundPseudomonas syringae is a widespread bacterial species complex that includes a number of significant plant pathogens. Amongst these, P. syringae pv. actinidiae (Psa) initiated a worldwide pandemic in 2008 on cultivars of Actinidia chinensis var. chinensis. To gain information about the expression of genes involved in pathogenicity we have carried out transcriptome analysis of Psa during the early stages of kiwifruit infection.ResultsGene expression in Psa was investigated during the first five days after infection of kiwifruit plantlets, using RNA-seq. Principal component and heatmap analyses showed distinct phases of gene expression during the time course of infection. The first phase was an immediate transient peak of induction around three hours post inoculation (HPI) that included genes that code for a Type VI Secretion System and nutrient acquisition (particularly phosphate). This was followed by a significant commitment, between 3 and 24 HPI, to the induction of genes encoding the Type III Secretion System (T3SS) and Type III Secreted Effectors (T3SE). Expression of these genes collectively accounted for 6.3% of the bacterial transcriptome at this stage. There was considerable variation in the expression levels of individual T3SEs but all followed the same temporal expression pattern, with the exception of hopAS1, which peaked later in expression at 48 HPI. As infection progressed over the time course of five days, there was an increase in the expression of genes with roles in sugar, amino acid and sulfur transport and the production of alginate and colanic acid. These are both polymers that are major constituents of extracellular polysaccharide substances (EPS) and are involved in biofilm production. Reverse transcription-quantitative PCR (RT-qPCR) on an independent infection time course experiment showed that the expression profile of selected bacterial genes at each infection phase correlated well with the RNA-seq data.ConclusionsThe results from this study indicate that there is a complex remodeling of the transcriptome during the early stages of infection, with at least three distinct phases of coordinated gene expression. These include genes induced during the immediate contact with the host, those involved in the initiation of infection, and finally those responsible for nutrient acquisition.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-5197-5) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Re-programming of Pseudomonas syringae pv. actinidiae gene expression during early stages of infection of kiwifruit

et al. 2018

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“…The proteins encoded by these genes are 92% identical, and the plasmid-located copy has been expressed heterologously and functionally characterized [37]. IAAL is postulated to convert free IAA into less active conjugate forms [38]. Heterologous expression of IAAL in tobacco and potato led to abnormal developmental changes [39].…”

Section: Resultsmentioning

confidence: 99%

Re-programming ofPseudomonas syringaepv.actinidiaegene expression during early stages of infection of kiwifruit

McAtee

Brian

Curran

et al. 2018

Preprint

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BackgroundPseudomonas syringae is a widespread bacterial species complex that includes a number of significant plant pathogens. Amongst these, P. syringae pv. actinidiae (Psa) initiated a worldwide pandemic in 2008 on cultivars of Actinidia chinensis var. chinensis. To gain information about the expression of genes involved in pathogenicity we have carried out transcriptome analysis of Psa during the early stages of kiwifruit infection.ResultsGene expression in Psa was investigated during the first five days after infection of kiwifruit plantlets, using RNA-seq. Principal component and heatmap analyses showed distinct phases of gene expression during the time course of infection. The first phase was an immediate transient peak of induction around three hours post inoculation (HPI) that included genes that code for a Type VI Secretion System and nutrient acquisition (particularly phosphate). This was followed by a significant commitment, between 3 and 24 HPI, to the induction of genes encoding the Type III Secretion System (T3SS) and Type III Secreted Effectors (T3SE). Expression of these genes collectively accounted for 6.3% of the bacterial transcriptome at this stage. There was considerable variation in the expression levels of individual T3SEs but all followed the same temporal expression pattern, with the exception of HopAS1, which peaked later in expression at 48 HPI. As infection progressed over the time course of five days, there was an increase in the expression of genes with roles in sugar, amino acid and sulfur transport and the production of alginate and colanic acid. These are both polymers that are major constituents of extracellular polysaccharide substances (EPS) and are involved in biofilm production. Reverse transcription-quantitative PCR (RT-qPCR) on an independent infection time course experiment showed that the expression profile of selected bacterial genes at each infection phase correlated well with the RNA-seq data.ConclusionsThe results from this study indicate that there is a complex remodeling of the transcriptome during the early stages of infection, with at least three distinct phases of coordinated gene expression. These include genes induced during the immediate contact with the host, those involved in the initiation of infection, and finally those responsible for nutrient acquisition.

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“…The likely reason for that is differences in the kinetics of the enzymatic reactions of auxin biosynthesis and metabolisation. There is some evidence that Yucca flavin-dependent monooxygenases, which catalyse the rate-limiting step in IAA synthesis, have slow kinetics [ 110 , 111 ], whereas IAA–amino synthases encoded by genes of the family GH3 are described as enzymes with fast kinetics [ 80 , 112 , 113 , 114 ].…”

Section: Discussionmentioning

confidence: 99%

Expression Analysis of Key Auxin Biosynthesis, Transport, and Metabolism Genes of Betula pendula with Special Emphasis on Figured Wood Formation in Karelian Birch

Tarelkina

Novitskaya

Галибина

et al. 2020

Plants

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Auxin status in woody plants is believed to be a critical factor for the quantity and quality of the wood formed. It has been previously demonstrated that figured wood formation in Karelian birch (Betula pendula Roth var. carelica (Merckl.) Hämet-Ahti) is associated with a reduced auxin level and elevated sugar content in the differentiating xylem, but the molecular mechanisms of the abnormal xylogenesis remained largely unclear. We have identified genes involved in auxin biosynthesis (Yucca), polar auxin transport (PIN) and the conjugation of auxin with amino acids (GH3) and UDP-glucose (UGT84B1) in the B. pendula genome, and analysed their expression in trunk tissues of trees differing in wood structure. Almost all the investigated genes were overexpressed in Karelian birch trunks. Although Yucca genes were overexpressed, trunk tissues in areas developing figured grain had traits of an auxin-deficient phenotype. Overexpression of GH3s and UGT84B1 appears to have a greater effect on figured wood formation. Analysis of promoters of the differentially expressed genes revealed a large number of binding sites with various transcription factors associated with auxin and sugar signalling. These data agree with the hypothesis that anomalous figured wood formation in Karelian birch may be associated with the sugar induction of auxin conjugation.

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Cloning and biochemical characterization of indole-3-acetic acid-amino acid synthetase PsGH3 from pea

Cited by 9 publications

References 47 publications

Re-programming of Pseudomonas syringae pv. actinidiae gene expression during early stages of infection of kiwifruit

Re-programming of Pseudomonas syringae pv. actinidiae gene expression during early stages of infection of kiwifruit

Re-programming ofPseudomonas syringaepv.actinidiaegene expression during early stages of infection of kiwifruit

Expression Analysis of Key Auxin Biosynthesis, Transport, and Metabolism Genes of Betula pendula with Special Emphasis on Figured Wood Formation in Karelian Birch

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