Cloning and analysis of the replication origin and the telomeres of the large linear plasmid pSLA2-L in Streptomyces rochei

Hiratsu, Keiichiro; Mochizuki, Susumu; Kinashi, Haruyasu

doi:10.1007/pl00008689

Cited by 38 publications

(50 citation statements)

References 33 publications

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“…SCP1, SLP2, pSLA2, pSLA2-L, pSCL1, pSCL2, pRL2, pRL4, pFRL2 and pSHK1) commonly contain one internal replication origin (Shiffman & Cohen, 1992;Chang et al, 1996;Redenbach et al, 1999;Huang et al, 2003;Hiratsu et al, 2000;Wu et al, 2006;Xu et al, 2006a;Zhang et al, 2009). Recently, Zhang et al (2010) found that 54 kb pFRL1 contained two replication loci.…”

Section: Discussionmentioning

confidence: 99%

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Three functional replication origins of the linear and artificially circularized plasmid SCP1 of Streptomyces coelicolor

et al. 2013

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Previous reports showed that the large linear plasmid SCP1 of Streptomyces coelicolor A3(2) contains a 5.4 kb centrally located replication locus. We report here that SCP1 actually contains three internal replication loci. Subcloning of the 5.4 kb sequence identified a 3.2 kb minimal locus (rep1A/repB/iteron) that determined propagation in Streptomyces lividans. The two newly identified replication genes, rep2A and rep3A, resembled the rep gene of Streptomyces circular plasmid pZL12. Transcription start points of the three replication genes were determined. The three replication loci could independently determine propagation in linear mode in S. lividans. Individual and sequential deletions of the rep1A and rep3A genes were successful. The SCP1-derived linear plasmids with deletions of the rep1A and/or rep3A genes still propagated in similar copy numbers, were inherited largely stable and were transferred efficiently by conjugation in S. coelicolor. Interestingly, SCP1 can be artificially circularized to yield a 280 kb circular plasmid, circular SCP-1 (C-SCP1), which contains the three replication loci. Strikingly, the copy numbers, inheritance and transfer of C-SCP1 resembled that of the linear SCP1 plasmids. Transcripts of the rep1A, rep2A and rep3A genes in linear or artificially circularized SCP1 were detected at all the time points of strain growth.

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Section: Discussionmentioning

confidence: 99%

“…Artificially circularized SCP1 at 621-284 635 bp by an aac (3) , 1999;Huang et al, 2003;Xu et al, 2006a;Zhang et al, 2009), while rep (hypothetical protein)/non-coding sequences (ncs) have been identified in pSLA2-L, pSCL2, pRL2, pRL4 and pFRL2 (Hiratsu et al, 2000;Wu et al, 2006;Zhang et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Three functional replication origins of the linear and artificially circularized plasmid SCP1 of Streptomyces coelicolor

et al. 2013

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“…Palindromes are important motifs that are involved in many cellular processes, such as DNA replication and gene regulation (Thukral et al, 1991;Hiratsu et al, 2000). To explore the change in palindrome abundance in pseudogenes during evolution, we compared palindrome abundances among the three pseudogene groups (see "MATERIALS AND METHODS" section) that differ in evolutionary distance.…”

Section: Evolution Of Palindrome Abundancementioning

confidence: 99%

Evolutionary direction of processed pseudogenes

Liu

Cui

Liang

et al. 2016

Sci. China Life Sci.

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While some pseudogenes have been reported to play important roles in gene regulation, little is known about the possible relationship between pseudogene functions and evolutionary process of pseudogenes, or about the forces responsible for the pseudogene evolution. In this study, we characterized human processed pseudogenes in terms of evolutionary dynamics. Our results show that pseudogenes tend to evolve toward: lower GC content, strong dinucleotide bias, reduced abundance of transcription factor binding motifs and short palindromes, and decreased ability to form nucleosomes. We explored possible evolutionary forces that shaped the evolution pattern of pseudogenes, and concluded that mutations in pseudogenes are likely determined, at least partially, by neighbor-dependent mutational bias and recombination-associated selection. GC content, mutual information, transcription factor binding motifs, mutational bias, recombination Citation:Liu, G

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“…The ooo autonomously replicating sequence of Streptomyces linear plasmids generally contains an origin of replication and one or more genes required for replication (24). These genes are located near the origin and may be subsidiary in some cases (25). Replication generates a 3' leading-strand overhang at the telomeres (Figure 1).…”

Section: Replication Of Linear Plasmidsmentioning

confidence: 99%

Linear plasmids and their replication

Ahsan

Kabir

2013

S.J. Microbiol.

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It is still a common belief that plasmids are circular. However, linear plasmids have been reported to exist more than a decade ago. Two types of linear plasmids are known. One type contains covalently closed ends and are commonly found in Borrelia, the causative agent of tick fever. The other type is characterized by the covalent attachment of proteins at the 5' ends and exists in a number of bacterial genera including Streptomyces, Rhodococcus, Mycobacterium and Planobispora. Recently, a linear plasmid in Salmonella enterica serovar Typhi of the Enterobacteriaceae family have been reported for the first time. This paper reviews various postulated mechanisms of replication of linear plasmids and focuses on the components of the replication machinery of linear plasmids studied to date.

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Cloning and analysis of the replication origin and the telomeres of the large linear plasmid pSLA2-L in Streptomyces rochei

Cited by 38 publications

References 33 publications

Three functional replication origins of the linear and artificially circularized plasmid SCP1 of Streptomyces coelicolor

Three functional replication origins of the linear and artificially circularized plasmid SCP1 of Streptomyces coelicolor

Evolutionary direction of processed pseudogenes

Linear plasmids and their replication

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