Cloning and analysis of the positively acting regulatory gene amdR from Aspergillus nidulans.

Andrianopoulos, Alex; Hynes, Michael J.

doi:10.1128/mcb.8.8.3532

Cited by 102 publications

(75 citation statements)

References 37 publications

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“…This is in contrast to the very low levels of transcription observed for the amdR regulatory gene (1). The abundance of the facB transcript is consistent with a gene that plays a structural as well as a regulatory role in acetate metabolism.…”

Section: Discussioncontrasting

confidence: 50%

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Isolation and analysis of the acetate regulatory gene, facB, from Aspergillus nidulans.

Katz¹,

Mj²

1989

Mol. Cell. Biol.

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The facB gene of Aspergillus nidulans is thought to be involved in acetate induction of enzymes required for acetate utilization and of the acetamidase encoded by the multiply regulated amdS gene. In addition, some evidence suggests that the facB gene has a structural as well as a regulatory role in acetate metabolism. The facB gene was cloned from a cosmid library by complementation of the facB101 loss-of-function mutation. Transformants receiving multiple copies of facB displayed stronger growth on acetamide media, indicating increased amdS expression, while growth on acetate was inhibited in these multicopy transformants. A 3.1-kilobase acetate-inducible facB transcript was detected by Northern (RNA) blot analysis. Examination of message levels in wild-type and mutant strains indicated that the facB gene is subject to carbon catabolite repression. Previous work has indicated that the presence of multiple copies of the 5' end of the amdS gene can result in titration of regulatory proteins. Additional copies of the facB gene were shown to specifically overcome the effect of facB product titration.

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Section: Discussioncontrasting

confidence: 50%

“…DNA-mediated transformation of A. nidulans has been described elsewhere (22 DNA and RNA preparation. Genomic DNA was prepared from A. nidulans by the method of Yelton et al (24) with the modifications described by Andrianopoulos and Hynes (1). RNA was prepared by a procedure developed for the isolation of N. crassa RNA (21).…”

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confidence: 99%

Isolation and analysis of the acetate regulatory gene, facB, from Aspergillus nidulans.

Katz¹,

Mj²

1989

Mol. Cell. Biol.

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“…These included 409 genes encoding antigens and cell-surface proteins (32; The Glycosylphosphatidylinositol (GPI) Lipid Anchor Project, http://mendel.imp.ac.at/gpi/gpi_genomes.html; and 38; The Allergome Project, http://www.allergome.org), 81 genes encoding allergens (references 38 [The Allergome Project, http://www.allergome.org] and 52), 319 genes encoding the major facilitator superfamily (MFS) and ATP-binding cassette (ABC) membrane transporter proteins (52,58; http://www .membranetransport.org/), 392 transcription factors (1,5,21,22,34,47,52,64,66,73,76), and the 383 genes present in 22 secondary metabolism A. fumigatus gene clusters (55).…”

Section: Methodsmentioning

confidence: 99%

Global Transcriptome Changes Underlying Colony Growth in the Opportunistic Human Pathogen Aspergillus fumigatus

et al. 2012

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Aspergillus fumigatus is the most common and deadly pulmonary fungal infection worldwide. In the lung, the fungus usually forms a dense colony of filaments embedded in a polymeric extracellular matrix. To identify candidate genes involved in this biofilm (BF) growth, we used RNA-Seq to compare the transcriptomes of BF and liquid plankton (PL) growth. Sequencing and mapping of tens of millions sequence reads against the A. fumigatus transcriptome identified 3,728 differentially regulated genes in the two conditions. Although many of these genes, including the ones coding for transcription factors, stress response, the ribosome, and the translation machinery, likely reflect the different growth demands in the two conditions, our experiment also identified hundreds of candidate genes for the observed differences in morphology and pathobiology between BF and PL. We found an overrepresentation of upregulated genes in transport, secondary metabolism, and cell wall and surface functions. Furthermore, upregulated genes showed significant spatial structure across the A. fumigatus genome; they were more likely to occur in subtelomeric regions and colocalized in 27 genomic neighborhoods, many of which overlapped with known or candidate secondary metabolism gene clusters. We also identified 1,164 genes that were downregulated. This gene set was not spatially structured across the genome and was overrepresented in genes participating in primary metabolic functions, including carbon and amino acid metabolism. These results add valuable insight into the genetics of biofilm formation in A. fumigatus and other filamentous fungi and identify many relevant, in the context of biofilm biology, candidate genes for downstream functional experiments.

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“…The amdR gene of A. nidulans is required for omega amino acid induction of both the gatA gene, encoding the ␥-aminobutyric acid (GABA) transaminase enzyme necessary for GABA catabolism, and the gabA gene, encoding the major GABA permease. Loss-offunction amdR mutations (e.g., amdR44) prevent the use of GABA as a sole carbon or nitrogen source (1,3,28). The gabA gene, unlike the gatA gene, is also regulated by nitrogen control via the areA gene product (9,28).…”

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The tamA gene of Aspergillus nidulans contains a putative zinc cluster motif which is not required for gene function

et al. 1996

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Expression of many nitrogen catabolic enzymes is controlled by nitrogen metabolite repression in Aspergillus nidulans. Although the phenotypes of tamA mutants have implicated this gene in nitrogen regulation, its function is unknown. We have cloned the tamA gene by complementation of a new tamA allele. The tamA sequence shares significant homology with the UGA35/DAL81/DURL gene of Saccharomyces cerevisiae. In vitro mutagenesis of sequences encoding a putative zinc cluster DNA binding domain indicated that this motif is not required for in vivo TamA function.

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Cloning and analysis of the positively acting regulatory gene amdR from Aspergillus nidulans.

Cited by 102 publications

References 37 publications

Isolation and analysis of the acetate regulatory gene, facB, from Aspergillus nidulans.

Isolation and analysis of the acetate regulatory gene, facB, from Aspergillus nidulans.

Global Transcriptome Changes Underlying Colony Growth in the Opportunistic Human Pathogen Aspergillus fumigatus

The tamA gene of Aspergillus nidulans contains a putative zinc cluster motif which is not required for gene function

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