Cloning and analysis of the K1 capsule biosynthesis genes of Escherichia coli: lack of homology with Neisseria meningitidis group B DNA sequences

Echarti, C.; Hirschel, Bernard; Boulnois, Graham J.; Varley, Jennifer; Waldvogel, Francis; Timmis, Kenneth N.

doi:10.1128/iai.41.1.54-60.1983

Cited by 54 publications

(13 citation statements)

References 28 publications

(13 reference statements)

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“…1973), may have the same origin according to the homology data of the sialyltransferases of these species, The common evolutionary origin of encapsulation is also supported by the fact that the molecular organization is similar in all these species (Kroll efa/.. 1989: Roberts e( a/.. 1988. Our homology data are in contrast to a previous report of Echarti et al {1983). who found no homologies between the cloned capsule genes of E coli K1 and DNA of N. meningitidis group B by Southern blot analysts.…”

Section: Discussioncontrasting

confidence: 99%

Evidence for a common molecular origin of the capsule gene loci in Gram‐negative bacteria expressing group II capsular polysaccharides

Frosch

Edwards

Bousset

et al. 1991

Molecular Microbiology

182

135

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Capsular polysaccharides of Gram-negative bacteria contribute to a large extent to the pathogenicity of these organisms. We show here that the molecular organization of the capsule gene loci in different serogroups of Neisseria meningitidis is similar to that of Haemophilus influenzae and Escherichia coli. A common molecular origin of the mechanisms of encapsulation is indicated by strong homology of the genes involved in transport of the capsular polysaccharides to the cell surface in all these organisms. The proteins involved in capsular polysaccharide transport fit the characteristics of ABC (ATP-binding cassette) transporters. Furthermore, by sequence comparison of the sialytransferases of N. meningitidis B and E. coli K1, the capsule of which is composed of alpha 2,8-linked polyneuraminic acid, a significant degree of homology was observed, indicating that the capsular polysaccharide type itself has the same evolutionary origin in these two pathogens.

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Section: Discussioncontrasting

confidence: 99%

Evidence for a common molecular origin of the capsule gene loci in Gram‐negative bacteria expressing group II capsular polysaccharides

Frosch

Edwards

Bousset

et al. 1991

Molecular Microbiology

182

135

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“…Because the type III capsule of GBS is a complex polysaccharide composed of 100 or more repeating units of four different monosaccharides, many genes may be involved in the biosynthesis of this extracellular polymer. In support of the polygenic nature of capsule biosynthesis, the molecular analysis of E. coli Ki capsule, a simple sialic acid polymer, has demonstrated that approximately 15 kb of DNA encoding at least 12 proteins is required for the synthesis, transport, and organization of the capsule on the cell surface (4,15,16). In addition, some of the Kl capsule genes share significant DNA homology with genes involved in the production of other K-type capsules in E. coli (12).…”

Section: Discussionmentioning

confidence: 99%

Molecular analysis of a region of the group B streptococcus chromosome involved in type III capsule expression

1989

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Type III group B streptococci (GBS) are the most common cause of neonatal sepsis and meningitis in the United States. The important role of the type III polysaccharide capsule and of the terminal sialic acid moiety of the capsule in the virulence of GBS has been demonstrated by using Tn916 mutagenesis. Several of the transposon insertion sites that resulted in defective type III capsule synthesis were located in a 30-kilobase (kb) region of the chromosome. Hybridization analysis of two other type III strains that differed in their relative virulence and of GBS serotypes Ia, Ib, Ic, and II showed that this region of the chromosome was highly conserved. A repetitive 1.4-kb sequence was found only in the 30-kb region of the more virulent type III strain, COH 1. The Escherichia coli maxicell in vivo expression system and an in vitro coupled transcriptiontranslation system successfully identified the proteins expressed from the 30-kb region. Comparison of the proteins expressed from the same DNA fragments in these two assays indicated that some of these proteins may contain leader sequences that would ultimately result in their secretion to the cell surface. Identification and further characterization of the genes and their products will provide the foundation for understanding the genetic and biochemical events in GBS capsular polysaccharide production.

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“…Gamma-delta (Tnl000) insertion (9) derivatives of pPL299 were isolated by using an F+-containing strain (RB308; Table 1) as described previously (6).…”

Section: Genetic Methods Mobilization Of Pkt231-based Hybridmentioning

confidence: 99%

Enzyme recruitment in vitro: use of cloned genes to extend the range of haloaromatics degraded by Pseudomonas sp. strain B13

Lehrbach¹,

Zeyer²,

Reineke³

et al. 1984

J Bacteriol

Self Cite

123

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DNA fragments containing the xylD and xylL genes of TOL plasmid pWW0-161 of Pseudomonas putida, which code for the catabolic enzymes toluate 1,2-dioxygenase and dihydrodihydroxybenzoic acid dehydrogenase, respectively, and the nahG gene of the NAH plasmid NAH7, which codes for salicylate hydroxylase, were cloned in pBR322 vector plasmid. Deletion and insertion mutagenesis were used to localize these genes with respect to crucial endonuclease cleavage sites. The pBR322-based plasmids were ligated to the broad host range cloning vector pKT231, or derivatives of it, and the hybrid plasmids were introduced into Pseudomonas sp. B13(WR1), a bacterium able to degrade 3-chlorobenzoate but not 4chlorobenzoate, 3,5-dichlorobenzoate, salicylate, or chlorosalicylates. The cloned xylD gene expanded the catabolic range of WRl to include 4-chlQrobenzoate, whereas the cloned xylD-xylL genes enabled the isolation of derivatives of WRl that degraded 3-chlorobenzoate, 4-chlorobenzoate, and 3,5-dichlorobenzoate. The cloned nahG gene extended the catabolic range of WRl to include salicylate and 3-, 4-, and 5-chlorosalicylate.

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Cloning and analysis of the K1 capsule biosynthesis genes of Escherichia coli: lack of homology with Neisseria meningitidis group B DNA sequences

Cited by 54 publications

References 28 publications

Evidence for a common molecular origin of the capsule gene loci in Gram‐negative bacteria expressing group II capsular polysaccharides

Evidence for a common molecular origin of the capsule gene loci in Gram‐negative bacteria expressing group II capsular polysaccharides

Molecular analysis of a region of the group B streptococcus chromosome involved in type III capsule expression

Enzyme recruitment in vitro: use of cloned genes to extend the range of haloaromatics degraded by Pseudomonas sp. strain B13

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