Cloning and analysis of the 5′-flanking region of rat Na+/K+-ATPase α1 subunit gene

Yagawa, Yuriko; Kawakami, Kiyoshi; Nagano, Kei

doi:10.1016/0167-4781(90)90099-n

Cited by 55 publications

(22 citation statements)

References 28 publications

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“…Conceivably, these second messengers induce transcription factors that may increase Na,KATPase subunit transcription by oxidant stress. The 5' flanking region of the rat Na,K-ATPase gene has been shown to contain multiple transcription factor binding consensus sequences, including: AP-1, stimulatory protein-1 (SP-1), and glucocorticoid response elements (GRE) [22,23]. We observed that the transcription factor jun is increased 6 h after recovery from HBO exposure and decreases gradually by 24 h of recovery ( fig.…”

Section: Discussionmentioning

confidence: 63%

Hyperbaric oxygenation upregulates rat lung Na,K-ATPase

et al. 1996

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H Hy yp pe er rb ba ar ri ic c o ox xy yg ge en na at ti io on n u up pr re eg gu ul la at te es s r ra at t l lu un ng g N Na a, ,K K--A AT TP Pa as se e In the present study, we tested whether short-term HBO, prior to inducing lung injury, would upregulate lung Na,K-ATPase.The results show that HBO, either intermittent or single 2.5 h exposure, increased lung Na,K-ATPase α-1 and β-1 messenger ribonucleic acid (mRNA) transcript levels up to fourfold. Na,K-ATPase activity in lungs of rats exposed to HBO increased twofold during the first 2 h following removal from the hyperbaric chamber, and remained elevated for up to 6 h following HBO.Conceivably, the increase in Na,K-ATPase activity following HBO is due to an increase in activity from a basal to a higher rate, or possibly due to recruitment/translocation of Na,K-ATPases from inner membranes to the plasma membrane.

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Section: Discussionmentioning

confidence: 63%

Hyperbaric oxygenation upregulates rat lung Na,K-ATPase

et al. 1996

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“…A more important explanation for the developmental differences in GC sensitivity may be the fact that the GC hormone receptor complex interacts with other transcriptional factors to regulate Na+,K+-ATPase mRNA and that the expression of these factors is tissue and age dependent. Binding domains for several transcriptional factors, such as AP-1, Sp 1, and CREB, have been identified in the promoter region of the Na+,K+-ATPase gene, in addition to GC-binding domains (21). The GC effect might be dependent on the expression of these factors.…”

Section: Discussionmentioning

confidence: 99%

Sensitive Periods for Glucocorticoids' Regulation of Na+,K+-ATPase mRNA in the Developing Lung and Kidney

et al. 1993

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ABSTRACT. We have previously reported that in the of Na+K+-ATPase in renal tissue from preweanling rats (10). The infant rat renal cortex, a saturating dose of glucocorticoid effect was so rapid (-20 min) that it is likely to be a direct effect hormones (GC) rapidly increases the abundance of Na+,K+-of the hormone on the transcription of Na'Kf-ATPase subunit ATPase mRNA. We now show that this effect is dose genes. dependent. In the renal cortex of 10-d-old rats, an increaseWe have now examined whether the GC effect is developin renal Na+,K+-ATPase mRNA occurs with 2.5 ~g beta-mentally regulated and, if so, whether the timing of the effect is methasone/100 g body weight. In subsequent experiments, tissue specific. Na'Kf-ATPase is expressed in all eukaryotic cells performed 6 h after a saturating dose (60 pg/100 g body and is of vital importance for the function of most tissues. We weight), we show that the effect is age dependent. The used tissue from lung and kidney, because these organs must most marked effects on renal cortical a-mRNA were found rapidly mature in the perinatal and the weaning periods, respecat 10 d of age (5.3-f 0.9-fold). A significant increase was tively. To evaluate the role of GC receptor availability, the effect also found in 20-d-old rats (1.6-+ 0.2-fold), but no effect of a single dose of betamethasone on renal mRNA was also was found in fetal and 5-d-old rats. Studies were also studied in adult adrenalectomized rats. performed on the lung, where the most marked effect was noted in the perinatal period (2.0-f 0.1-fold 2 d before MATERIALS AND METHODS birth and 1.76 + 0.2 at 5 d of age), but no effect on amRNA was found at 10 and 20 d. In one protocol, the Animals. Experiments were performed on Sprague-Dawley rats effect of betamethasone on renal Na+K+-ATPase mRNAs (ALAB, Sollentuna, Sweden). Timed pregnant rats were housed abundance was determined in adult adrenalectomized rats. individually and given free access to food and water. On gestaIn these rats, betamethasone induced a significant 1.6-+ tional d 18 and 20 (i.e. 4 and 2 d before expected parturition), 0.2-fold and 1.8-f 0.3-fold increase in renal Na+,K+-the rats received an intraperitoneal injection of betamethasone ATPase mRNA. This effect, however, was significantly (Glaxo Labs, Ltd., Greenford, England) (60 pg/100 g body smaller than the increase induced in intact 10-d-old rats. weight) or vehicle. Six h after the injection, betamethasone-and GC induction of Na+,K+-ATPase mRNA is age and tissue vehicle-treated rats were anesthetized with thiobutabarbital (8 dependent and is dependent on factors other than GC-mg/100 g body weight). Thereafter, fetuses were extracted within receptor availability. The GC-sensitive period appears to 10-15 min, and the fetal lungs and kidneys were removed. Renal coincide with the physiologic need for organ maturation. cortex was rapidly dissected on ice. We pooled the lungs and (Pediatr Res 33: 5-9,1993) renal cortices from three to five fetuses before homogenization. Infant rats were kept with...

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“…), 5 (Chau et al 1992), 6 (Braidotti et al 1993), Z (Kreysing et al 1990), 8(Mitsubuchi et al 1991, 9 (Forrest et al 1991), t° (Tasanen et al 1992), ~ (Giallongo et al 1990), ~2 (Das et al 1993), la (Jenkins et al 1991), 14(Katz andKahana 1988), lS (Moshier et al 1992), 16 (Wen et al 1989), lZ (Amy et al 1990), 18 (Yagawa et al 1990), W (Miyake et al 1986), 2° (Cavailles et al 1993), 21 (Miyatake et al 1985), 22 (Degen et al 1991), 2a{Zhou et al 1992), ~4 (Tewari et al 1989), 2S (Mamula and Goldfine 1992), 26 (Lutfalla et al 1992), 27 (Haendler andHofer 1990), 28{Kutt et al 1989), 29 (Nojima 1989), S° (Parmentier and Lefort 1991), al (Tsukamoto et al 1991), a2 (Lee et al 1992), a3 (Khew-Goodall et al 1991), 34 (Kurihara et al 1990), aS (Pearson et al 1991), 36{Kunze et al 1991), 37 (Baer et al 1990), aa{Biamonti et al 1989, 39 Perry 1985), 4°{Hurt et al 1989), 4~ (Sittman et al 1983), 42 (Furuno et al 1991), 43 (Ku et al 1993), 44{Adachi and Tsujimoto 1990), 45 (Park et al 1992), 46 (Luethy et al 1990), 47 (Walter et al 1989), 48 …”

Section: Genes and Developmentmentioning

confidence: 99%

NRF-1, an activator involved in nuclear-mitochondrial interactions, utilizes a new DNA-binding domain conserved in a family of developmental regulators.

Virbasius¹,

Virbasius²,

Scarpulla³

1993

Genes & Development

312

253

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Nuclear respiratory factor 1 (NRF-1) was first discovered as an activator of the cytochrome c gene and was subsequently found to play a broader role in nuclear-mitochondrial interactions. We have now cloned a HeLa cDNA encoding NRF-1 using degenerate oligomers derived from tryptic peptide sequences for PCR amplification. The cDNA-encoded protein was indistinguishable from the authentic HeLa cell factor on denaturing gels, displayed the expected NRF-1 DNA-binding specificity, and made the same guanine nucleotide contacts as HeLa NRF-1 on binding known NRF-1 recognition sites. Antiserum raised against the highly purified recombinant protein recognized the identical DNA-protein complex formed using either a crude nuclear fraction or nearly homogeneous HeLa NRF-1. Recombinant NRF-1 also activated transcription through specific sites from several NRF-l-responsive promoters, confirming both the transcriptional activity and specificity of the cDNA product. Portions of NRF-1 are closely related to sea urchin P3A2 and the erect wing (EWG) protein of Drosophila. Both are recently identified developmental regulatory factors. The region of highest sequence identity with P3A2 and EWG was in the amino-terminal half of the molecule, which was found by deletion mapping to contain the DNA-binding domain, whereas the carboxy-terminal half of NRF-1 was highly divergent from both proteins. The DNA-binding domain in these molecules is unrelated to motifs found commonly in DNA-binding proteins; thus, NRF-1, P3A2, and EWG represent the founding members of a new class of highly conserved sequence-specific regulatory factors.

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Cloning and analysis of the 5′-flanking region of rat Na+/K+-ATPase α1 subunit gene

Cited by 55 publications

References 28 publications

Hyperbaric oxygenation upregulates rat lung Na,K-ATPase

Hyperbaric oxygenation upregulates rat lung Na,K-ATPase

Sensitive Periods for Glucocorticoids' Regulation of Na+,K+-ATPase mRNA in the Developing Lung and Kidney

NRF-1, an activator involved in nuclear-mitochondrial interactions, utilizes a new DNA-binding domain conserved in a family of developmental regulators.

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