Cloning and analysis of cDNAs encoding the hypusine‐containing protein eIF5A of two lepidopteran insect species

Oers, Monique M. van; Marwijk, M. van; Kwa, M.S.G.; Vlak, Just M.; Thomas, Adri A. M.

doi:10.1046/j.1365-2583.1999.00148.x

Cited by 23 publications

(23 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The AcMNPV ie1 gene, included as a control for virus infection, was expressed in all the virus-infected larvae, but not in the mock-infected ones. The S. exigua eIF5A gene, encoding eukaryotic translation initiation factor 5A [46], was included as a host control and showed expression in both mock- and virus-infected larvae.…”

Section: Resultsmentioning

confidence: 99%

Protein Tyrosine Phosphatase-Induced Hyperactivity Is a Conserved Strategy of a Subset of BaculoViruses to Manipulate Lepidopteran Host Behavior

et al. 2012

Self Cite

View full text Add to dashboard Cite

Many parasites manipulate host behavior to increase the probability of transmission. To date, direct evidence for parasitic genes underlying such behavioral manipulations is scarce. Here we show that the baculovirus Autographa californica nuclear polyhedrovirus (AcMNPV) induces hyperactive behavior in Spodoptera exigua larvae at three days after infection. Furthermore, we identify the viral protein tyrosine phosphatase (ptp) gene as a key player in the induction of hyperactivity in larvae, and show that mutating the catalytic site of the encoded phosphatase enzyme prevents this induced behavior. Phylogenetic inference points at a lepidopteran origin of the ptp gene and shows that this gene is well-conserved in a group of related baculoviruses. Our study suggests that ptp-induced behavioral manipulation is an evolutionarily conserved strategy of this group of baculoviruses to enhance virus transmission, and represents an example of the extended phenotype concept. Overall, these data provide a firm base for a deeper understanding of the mechanisms behind baculovirus-induced insect behavior.

show abstract

Section: Resultsmentioning

confidence: 99%

Protein Tyrosine Phosphatase-Induced Hyperactivity Is a Conserved Strategy of a Subset of BaculoViruses to Manipulate Lepidopteran Host Behavior

et al. 2012

Self Cite

View full text Add to dashboard Cite

show abstract

“…Previous studies have shown that mRNA levels of actin, histones, heat shock protein 70 (46), and the translation initiation factors eIF4E (57) and eIF5A (56) were substantially reduced from 12 to 24 h following infection with AcMNPV. In contrast, a dramatic increase in TBP was observed in AcMNPV-infected TN-368 and Sf21 cells (50); those authors also showed that TBP localized to viral DNA replication centers at the late stage of infection, suggesting a role for TBP during late viral transcription.…”

Section: Discussionmentioning

confidence: 95%

ERK- and JNK-Dependent Signaling Pathways Contribute to Bombyx mori Nucleopolyhedrovirus Infection

Katsuma

Mita

Shimada

2007

J Virol

111

View full text Add to dashboard Cite

Mitogen-activated protein kinases (MAPKs) often play important roles in virus infection. To explore intracellular signaling pathways induced by baculovirus infection, we examined the involvement of MAPKs inBombyx mori nucleopolyhedrovirus (BmNPV) infection of BmN cells. We found that specific inhibitors of extracellular signal-regulated kinase (ERK) kinase and c-Jun NH 2 -terminal kinase (JNK) significantly reduced occlusion body (OB) formation and budded virus (BV) production. Next, we quantified OB and BV production after applying the inhibitors at different times postinfection (p.i.). The inhibitors significantly reduced OB and BV production to various extents when applied at 12 h p.i., indicating that the reduction of BmNPV infectivity by these inhibitors occurs at the late stage of infection. Also, we observed that these inhibitors markedly repressed or deregulated the expression of delayed early, late, and very late gene products. Western blot analysis using phospho-MAPK-specific antibodies showed that ERK and JNK were activated at the late stage of BmNPV infection. In addition, the magnitude and pattern of MAPK activation were dependent on the multiplicity of infection. To verify the effects of the inhibitors on BmNPV infection, we also attempted to knock down the B. mori genes BmErk and BmJnk, which encode ERK and JNK, respectively. Knockdown of BmErk and BmJnk resulted in the reduced production of OBs and BVs, confirming that BmERK and BmJNK are involved in the BmNPV infection process. Taken together, these results indicate that the activation of MAPK signaling pathways is required for efficient infection by BmNPV.

show abstract

“…They showed by Northern blot analysis that mRNA levels of actin, histone and heat shock protein 70 were substantially reduced from 12 to 18 h following infection with AcMNPV. More recently, expression levels of Sf21 translation initiation factors eIF4E (Van Oers et al, 2001) and eIF5A (Van Oers et al, 1999) were also shown to be down-regulated at the mRNA level following infection with AcMNPV, despite the reliance of the virus on the host translation machinery to produce its viral proteins. This decrease in mRNA levels was found to begin between 12 and 24 h after infection.…”

Section: Introductionmentioning

confidence: 99%

Autographa californica nucleopolyhedrovirus infection of Spodoptera frugiperda cells: a global analysis of host gene regulation during infection, using a differential display approach

Nobiron

O’Reilly

Olszewski

2003

Journal of General Virology

View full text Add to dashboard Cite

Autographa californica nucleopolyhedrovirus (AcMNPV), the type member of the virus family Baculoviridae, infects pest insects and has been the subject of many studies for its development as a biopesticide. It is also the virus upon which most of the commercial baculovirus protein expression systems are based. AcMNPV infection of cultured host Spodoptera frugiperda (Sf9) cells can induce a number of alterations of host cell properties including altering the cellular cytoskeleton, an arrest of the cell cycle in G 2 /M, and the global shutoff of host protein translation. Additionally, several cellular transcripts have been shown to be down-regulated following AcMNPV infection. In this study, we take a differential display approach to address whether a global down-regulation of Sf9 host transcripts occurs at late times of infection. Additionally, we also use this approach to search for host mRNAs which are up-regulated at early times of infection, and may be important for facilitating baculovirus infection. From these experiments we can confirm a global down-regulation of Sf9 mRNA levels at late times of infection. We also found that up-regulation of individual host gene RNA levels at early times of infection did not occur frequently. One host transcript which was found to be transiently up-regulated as a result of AcMNPV infection was an Sf9 Hsc70 gene. Hsc70 proteins have been shown to play a vital role in the life-cycle of other large DNA viruses, which suggests that this protein is also important for baculovirus infection.

show abstract

Cloning and analysis of cDNAs encoding the hypusine‐containing protein eIF5A of two lepidopteran insect species

Cited by 23 publications

References 33 publications

Protein Tyrosine Phosphatase-Induced Hyperactivity Is a Conserved Strategy of a Subset of BaculoViruses to Manipulate Lepidopteran Host Behavior

Protein Tyrosine Phosphatase-Induced Hyperactivity Is a Conserved Strategy of a Subset of BaculoViruses to Manipulate Lepidopteran Host Behavior

ERK- and JNK-Dependent Signaling Pathways Contribute to Bombyx mori Nucleopolyhedrovirus Infection

Autographa californica nucleopolyhedrovirus infection of Spodoptera frugiperda cells: a global analysis of host gene regulation during infection, using a differential display approach

Contact Info

Product

Resources

About