Cloning and analysis of bile salt hydrolase genes from Lactobacillus plantarum CGMCC No. 8198

Gu, Xiangchao; Luo, Xue‐Gang; Wang, Chong-Xi; Ma, De-Yun; Wang, Yan; He, Yingying; Li, Wen; Zhou, Hao; Zhang, Tongcun

doi:10.1007/s10529-013-1434-9

Cited by 54 publications

(36 citation statements)

References 26 publications

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“…Kumar et al (2013) studied the characteristics of recombinant (r) BSH of L. fermentum NCDO394 and identified that purified rBSH enzyme hydrolyzed six major bile substrates, with a special preference toward glycine-conjugated bile salts and exhibited maximal activity against GCA. Previous studies also confirmed that BSHs from Lactobacillus spp., including L. salivarius (Wang et al, 2012), L. plantarum CGMCC 8198 (Gu et al, 2014), and BSH-C of L. johnsonii PF01 (Chae et al, 2013), had a substrate specificity toward glyco-conjugated bile salts. Biochemical characterization studies revealed that the pH and temperature optima of Lg BSH were, respectively, found to be 5.5 and 52°C.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Bile Salt Hydrolase from Lactobacillus gasseri FR4 and Demonstration of Its Substrate Specificity and Inhibitory Mechanism Using Molecular Docking Analysis

2017

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Probiotic bacteria are beneficial to the health of poultry animals, thus are used as alternative candidates for antibiotics used as growth promoters (AGPs). However, they also reduce the body weight gain due to innate bile salt hydrolase (BSH) activity. Hence, the addition of a suitable BSH inhibitor along with the probiotic feed can decrease the BSH activity. In this study, a BSH gene (981 bp) encoding 326-amino acids was identified from the genome of Lactobacillus gasseri FR4 (LgBSH). The LgBSH-encoding gene was cloned and purified using an Escherichia coli BL21 (DE3) expression system, and its molecular weight (37 kDa) was confirmed by SDS–PAGE and a Western blot analysis. LgBSH exhibited greater hydrolysis toward glyco-conjugated bile salts compared to tauro-conjugated bile salts. LgBSH displayed optimal activity at 52°C at a pH of 5.5, and activity was further increased by several reducing agents (DTT), surfactants (Triton X-100 and Tween 80), and organic solvents (isopropanol, butanol, and acetone). Riboflavin and penicillin V, respectively, inhibited LgBSH activity by 98.31 and 97.84%. A homology model of LgBSH was predicted using EfBSH (4WL3) as a template. Molecular docking analysis revealed that the glycocholic acid had lowest binding energy of -8.46 kcal/mol; on the other hand, inhibitors, i.e., riboflavin and penicillin V, had relatively higher binding energies of -6.25 and -7.38 kcal/mol, respectively. Our results suggest that L. gasseri FR4 along with riboflavin might be a potential alternative to AGPs for poultry animals.

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Section: Discussionmentioning

confidence: 99%

Characterization of Bile Salt Hydrolase from Lactobacillus gasseri FR4 and Demonstration of Its Substrate Specificity and Inhibitory Mechanism Using Molecular Docking Analysis

2017

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“…BSH deconjugation activity is well represented among intestinal microbial genera 10,22 , it is predominantly characterized among gram-positive species Bifidobacterium [23][24][25] , Clostridium 26,27 , Enterococcus 28,29 , Listeria 30 , and Lactobacillus [31][32][33][34][35][36][37][38] , including Lb. plantarum species [39][40][41][42][43] . In addition, several investigators reported that bile tolerance is a strain-specific trait and tolerances of species cannot be generalized within a species or a genus 15,16 .…”

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confidence: 99%

Beneficial bile acid metabolism from Lactobacillus plantarum of food origin

Prete

Long

Gallardo

et al. 2020

Sci Rep

102

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Bile acid (BA) signatures are altered in many disease states. BA metabolism is an important microbial function to assist gut colonization and persistence, as well as microbial survival during gastro intestinal (GI) transit and it is an important criteria for potential probiotic bacteria. Microbes that express bile salt hydrolase (BSH), gateway BA modifying enzymes, are considered to have an advantage in the gut. This property is reported as selectively limited to gut-associated microbes. Food-associated microbes have the potential to confer health benefits to the human consumer. Here, we report that food associated Lactobacillus plantarum strains are capable of BA metabolism, they can withstand BA associated stress and propagate, a recognised important characteristic for GIT survival. Furthermore, we report that these food associated Lactobacillus plantarum strains have the selective ability to alter BA signatures in favour of receptor activation that would be beneficial to humans. Indeed, all of the strains examined showed a clear preference to alter human glycol-conjugated BAs, although clear strain-dependent modifications were also evident. This study demonstrates that BA metabolism by food-borne nonpathogenic bacteria is beneficial to both microbe and man and it identifies an evolutionary-conserved characteristic, previously considered unique to gut residents, among food-associated non-pathogenic isolates.

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“…The intricate interconnection of the genera Lactobacillus [102][103][104][105][106] Enterococcus [107,108], Bifidobacterium [109,110], Clostridium [111], Bacteroides [112], Peptostreptococcus [113] regulates primary bile salt synthesis and secondary bile salt metabolism of the host [114]. Growth-promoting mechanism of most subtherapeutic dose antibiotics can be related to decreased activity of the bile salt hydrolase (BSH) enzyme that catalyses deconjugation of bile salts [115].…”

Section: Discussionmentioning

confidence: 99%

Nutraceuticals Induced Structural Changes in Broiler Gastrointestinal Tract Microbiota

Tolnai¹,

Fauszt²,

Fidler³

et al. 2020

Preprint

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Background The effects of nutraceuticals on the modulation of the intestinal microbiota are receiving increased attention however there are scarce number of studies investigating their effects in broiler meat production. The aim of this study was to implement feeding strategies and carry out a comprehensive trial on the interplay between carotenoids, anthocyanins, fructo-oligosaccharides, probiotics and gastrointestinal tract microbiota. Our feeding program was applied on an intensive production system with 1080 broiler Ross 308 flock.Results We observed that nutraceuticals and synbiotics did not affect growth performance remarkably nevertheless, positive correlation was found between body weight and the beneficious Bacteroidales, Corynebacteriales and Pseudomonadales. Nutraceuticals were shown to boost broiler intestinal diversity and differentially enriched Lactobacillus, Enterococcus, Streptococcus and Escherichia-Shigella in core microbiome during the different stages of broiler rearing. Furthermore, diet supplemented with different nutraceuticals was shown to increase the number of unique beneficial bacteria (Faecalibacterium, Akkermansia).Conclusion We concluded that nutraceutical-based feeding strategies can offer a promising, green approach for intensive poultry rearing by improving the health and production of livestock. We believe that the conversion of food industrial waste would result more sustainable bioactive component rich forages which invention could be implemented by other commercial antibiotic-free animal production system providing safe and quality meat.

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Cloning and analysis of bile salt hydrolase genes from Lactobacillus plantarum CGMCC No. 8198

Cited by 54 publications

References 26 publications

Characterization of Bile Salt Hydrolase from Lactobacillus gasseri FR4 and Demonstration of Its Substrate Specificity and Inhibitory Mechanism Using Molecular Docking Analysis

Characterization of Bile Salt Hydrolase from Lactobacillus gasseri FR4 and Demonstration of Its Substrate Specificity and Inhibitory Mechanism Using Molecular Docking Analysis

Beneficial bile acid metabolism from Lactobacillus plantarum of food origin

Nutraceuticals Induced Structural Changes in Broiler Gastrointestinal Tract Microbiota

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