Cloning and analysis of a gene cluster from Streptomyces coelicolor that causes accelerated aerial mycelium formation in Streptomyces lividans

Ma, Hongwen; Kendall, Kevin

doi:10.1128/jb.176.12.3800-3811.1994

Cited by 54 publications

(59 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Streptomyces lividans requires a large amount of copper for proper aerial growth (243). This defect can be rescued by enhanced expression of the ram cluster (196), which ultimately leads to the production of the surfactant SapB (see the next section). Recently, Sébastien Rigali and colleagues showed that development can be restored to bldJ and bldK mutants by supplementing R2YE agar with iron (244).…”

Section: Environmental Control Of Aerial Hypha Formationmentioning

confidence: 99%

“…Several new classes of developmental genes have been identified on the basis of physiological criteria, such as the acceleration of aerial mycelium formation in S. lividans (ram genes, for rapid aerial mycelium [196]), complementation of mutants of S. griseus with disturbed sporulation (ssgA-like genes, for sporulation of Streptomyces griseus [197]), or disruptions in sugar metabolism (186,198,199).…”

Section: The Streptomyces Life Cyclementioning

confidence: 99%

“…SapB-type proteins are widespread in streptomycetes; well-studied examples include AmfS in S. griseus and SapT in S. tendae (265, 266). In S. coelicolor, SapB is encoded by the ramS gene in the ramCSAB gene cluster (196,267), which is controlled by the orphan response regulatory gene ramR (268-270). In turn, at least in S. griseus, transcription of the amf operon, and thus of amfS, depends on AdpA and therefore ultimately also on BldA (215).…”

Section: Facilitating Aerial Growth: the Roles Of Chaplins Rodlins mentioning

confidence: 99%

See 2 more Smart Citations

Taxonomy, Physiology, and Natural Products of Actinobacteria

Barka

Vatsa

Sanchez

et al. 2016

Microbiol Mol Biol Rev

1,550

1,048

View full text Add to dashboard Cite

SUMMARYActinobacteriaare Gram-positive bacteria with high G+C DNA content that constitute one of the largest bacterial phyla, and they are ubiquitously distributed in both aquatic and terrestrial ecosystems. ManyActinobacteriahave a mycelial lifestyle and undergo complex morphological differentiation. They also have an extensive secondary metabolism and produce about two-thirds of all naturally derived antibiotics in current clinical use, as well as many anticancer, anthelmintic, and antifungal compounds. Consequently, these bacteria are of major importance for biotechnology, medicine, and agriculture.Actinobacteriaplay diverse roles in their associations with various higher organisms, since their members have adopted different lifestyles, and the phylum includes pathogens (notably, species ofCorynebacterium,Mycobacterium,Nocardia,Propionibacterium, andTropheryma), soil inhabitants (e.g.,MicromonosporaandStreptomycesspecies), plant commensals (e.g.,Frankiaspp.), and gastrointestinal commensals (Bifidobacteriumspp.).Actinobacteriaalso play an important role as symbionts and as pathogens in plant-associated microbial communities. This review presents an update on the biology of this important bacterial phylum.

show abstract

Section: Environmental Control Of Aerial Hypha Formationmentioning

confidence: 99%

Section: The Streptomyces Life Cyclementioning

confidence: 99%

Section: Facilitating Aerial Growth: the Roles Of Chaplins Rodlins mentioning

confidence: 99%

See 1 more Smart Citation

Taxonomy, Physiology, and Natural Products of Actinobacteria

Barka

Vatsa

Sanchez

et al. 2016

Microbiol Mol Biol Rev

1,550

1,048

View full text Add to dashboard Cite

show abstract

“…This strategy has led to the cloning of a gene cluster including amfR, amfA, and amfB (31). A similar gene cluster from S. coelicolor A3 (2) has been cloned as a DNA fragment that causes accelerated aerial mycelium formation in Streptomyces lividans (22). An additional gene which we cloned by this strategy was orf1590, which was shown by Babcock and Kendrick (1,2) to complement one class of mutants of S. griseus.…”

Section: Members Of the Gram-positive Bacterial Genus Streptomycesmentioning

confidence: 99%

Cloning and characterization of a gene involved in aerial mycelium formation in Streptomyces griseus

et al. 1995

View full text Add to dashboard Cite

, S. griseus HH1, was cloned from this strain on a high-copy-number plasmid. Subcloning and nucleotide sequencing revealed that one open reading frame with 218 amino acids, named AmfC, served as a multicopy suppressor of the aerial mycelium-defective phenotype of the A-factor-deficient strain. The amfC gene did not restore A-factor or streptomycin production, indicating that amfC is involved in aerial mycelium formation independently of secondary metabolic function. Disruption of the chromosomal amfC gene in the wild-type S. griseus strain caused a severe reduction in the abundance of spores but no effect on the shape or size of the spores. The infrequent sporulation of the amfC disruptant was reversed by introduction of amfC on a plasmid. The amfC-defective phenotype was also restored by the orf1590 gene but not by the amfR-amfA-amfB gene cluster. Nucleotide sequences homologous to the amfC gene were distributed in all of 12 Streptomyces species tested, including Streptomyces coelicolor A3(2). The amfC homolog of S. coelicolor A3(2) was cloned and its nucleotide sequence was determined. The AmfC products of S. griseus and S. coelicolor A3(2) showed a 60% identity in their amino acid sequences. Introduction of the amfC gene of S. coelicolor A3(2) into strain HH1 induced aerial mycelium formation and sporulation, which suggests that both play the same functional role in morphogenesis in the strains.

show abstract

“…The ram genes were discovered by virtue of their ability to induce rapid aerial mycelium formation when overexpressed in the wild type (32), and they have recently been shown to specify production of the morphogenetic peptide SapB (29). SapB is a 21-amino-acid peptide that plays a vital role in aerial mycelium formation during growth on rich medium (16,26,51,52).…”

mentioning

confidence: 99%

ThebldCDevelopmental Locus ofStreptomyces coelicolorEncodes a Member of a Family of Small DNA-Binding Proteins Related to the DNA-Binding Domains of the MerR Family

et al. 2005

View full text Add to dashboard Cite

The bldC locus, required for formation of aerial hyphae in Streptomyces coelicolor, was localized by map-based cloning to the overlap between cosmids D17 and D25 of a minimal ordered library. Subcloning and sequencing showed that bldC encodes a member of a previously unrecognized family of small (58-to 78-residue) DNAbinding proteins, related to the DNA-binding domains of the MerR family of transcriptional activators. BldC family members are found in a wide range of gram-positive and gram-negative bacteria. Constructed ⌬bldC mutants were defective in differentiation and antibiotic production. They failed to form an aerial mycelium on minimal medium and showed severe delays in aerial mycelium formation on rich medium. In addition, they failed to produce the polyketide antibiotic actinorhodin, and bldC was shown to be required for normal and sustained transcription of the pathway-specific activator gene actII-orf4. Although ⌬bldC mutants produced the tripyrrole antibiotic undecylprodigiosin, transcripts of the pathway-specific activator gene (redD) were reduced to almost undetectable levels after 48 h in the bldC mutant, in contrast to the bldC ؉ parent strain in which redD transcription continued during aerial mycelium formation and sporulation. This suggests that bldC may be required for maintenance of redD transcription during differentiation. bldC is expressed from a single promoter. S1 nuclease protection assays and immunoblotting showed that bldC is constitutively expressed and that transcription of bldC does not depend on any of the other known bld genes. The bldC18 mutation that originally defined the locus causes a Y49C substitution that results in instability of the protein.

show abstract

Cloning and analysis of a gene cluster from Streptomyces coelicolor that causes accelerated aerial mycelium formation in Streptomyces lividans

Cited by 54 publications

References 51 publications

Taxonomy, Physiology, and Natural Products of Actinobacteria

Taxonomy, Physiology, and Natural Products of Actinobacteria

Cloning and characterization of a gene involved in aerial mycelium formation in Streptomyces griseus

ThebldCDevelopmental Locus ofStreptomyces coelicolorEncodes a Member of a Family of Small DNA-Binding Proteins Related to the DNA-Binding Domains of the MerR Family

Contact Info

Product

Resources

About