1985
DOI: 10.1128/mcb.5.3.563
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Cloned mouse DNA fragments can replicate in a simian virus 40 T antigen-dependent system in vivo and in vitro.

Abstract: Mouse liver DNA was cut out with BamHI and cloned into YIp5, which contained the URA3 gene of Saccharomyces cerevisiae in pBR322. Of the several plasmids isolated, two plasmids, pMU65 and pMU1l1, could transform S. cerevisiae from the URA-to the URA+ phenotype and could replicate autonomously within the transformant, indicating that mouse DNA fragments present in pMU65 or pMU111 contain autonomously replicating sequences (ARS) for replication in S. cerevisiae. Furthermore, to determine the correlation between … Show more

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Cited by 12 publications
(6 citation statements)
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“…Autonomous replication of isolated mammalian sequences has also been reported (1,31); however, autonomous replication of mammalian DNA shown to contain an OBR by biochemical methods (12,13,20,27) has not yet been observed. Our data strongly indicate that origins which initiate the replication of chromosomal sequences can also initiate the replication of extrachromosomal sequences.…”
Section: Discussionmentioning
confidence: 99%
“…Autonomous replication of isolated mammalian sequences has also been reported (1,31); however, autonomous replication of mammalian DNA shown to contain an OBR by biochemical methods (12,13,20,27) has not yet been observed. Our data strongly indicate that origins which initiate the replication of chromosomal sequences can also initiate the replication of extrachromosomal sequences.…”
Section: Discussionmentioning
confidence: 99%
“…It is not yet clear whether SV40 T-antigen and the ori sequence are required for replication in embryos. Thus, SV40 replication may represent either a low level of T-antigen-dependent viral DNA replication that results from the large number of viral DNA molecules injected or it may be analogous to replication of a cellular o(i sequence similar to the one described by Ariga et al (3).…”
Section: -Cellmentioning
confidence: 99%
“…With the advent of soluble systems capable of initiating SV40 DNA replication in vitro (21)(22)(23)(24), it was possible to determine which DNA sequence exhibited the strongest affinity for replication initiation factors by its ability to inhibit replication of a second plasmid containing the SV40 oriregion. Surprisingly, the DNA binding site for a limiting factor(s) required to initiate SV40 DNA replication did not coincide with ori-core and did not include the strongest T-Ag binding sequences, although it was specific for SV40.…”
mentioning
confidence: 99%