Cloned cDNA Probes for the Detection of Tomato Spotted Wilt Virus

Ronco, Alicia Estela; Bó, E. Dal; Ghiringhelli, P. D.; Medrano, Celia; Romanowski, Vı́ctor; Sarachu, Alberto N.; Grau, Oscar

doi:10.1094/phyto-79-1309

Cited by 17 publications

(4 citation statements)

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“…Huguenot et al (1990) compared the usefulness of different ELISA procedures using MAbs with a molecular hybridization technique using riboprobes for the detection of TSWV. The use of riboprobes and also of cDNA (Ronco et al, 1989) has not yet been widely applied.…”

Section: Introductionmentioning

confidence: 99%

Serological Differentiation of 20 Isolates of Tomato Spotted Wilt Virus

Ávila

Huguenot

Resende

et al. 1990

Journal of General Virology

133

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Twenty tomato spotted wilt virus (TSWV) isolates were serologically compared in ELISA employing five different procedures using a rabbit polyclonal antiserum against nucleocapsid proteins (NuAb R) and mouse monoclonal antibodies (MAbs), two directed to nucleocapsid proteins (N1 and N2) and four directed to glycoproteins G1 to G4. All the antisera were raised against TSWV-CNPH~. The 20 isolates were differentiated into two distinct serogroups. Serogroup I consisting of 16 isolates strongly reacted with NuAb R. The other four isolates were poorly recognized by NuAb ~ and were placed in another serogroup, designated II. The panel of MAbs differentiated the TSWV isolates into three serotypes. The 16 isolates forming serogroup I reacted strongly with the MAbs generated and were identified as serotype I isolates. The four isolates which made up serogroup II were split into serotypes II and III. The serotype II isolates did not respond or responded poorly with MAbs N1, N2 and G3. The two other isolates placed in serotype III were recognized by N 1 but not by N2 and G3. Two isolates became defective after several mechanical passages and failed to respond or responded very poorly with MAbs directed to glycoproteins. Our results show that ELISA employing polyclonal and monoclonal antisera is a useful tool to differentiate TSWV isolates and to detect defective forms. The results also strongly suggest that TSWV nucleocapsid proteins are less conserved than the glycoproteins.

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Section: Introductionmentioning

confidence: 99%

Serological Differentiation of 20 Isolates of Tomato Spotted Wilt Virus

Ávila

Huguenot

Resende

et al. 1990

Journal of General Virology

133

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“…Como ocurrió con muchos otros patógenos vegetales, el desarrollo de técnicas moleculares brinda amplias oportunidades y ha permitido mejorar el diagnóstico y profundizar en el conocimiento de estos virus. Desde sus inicios con el uso de sondas de cADN (Cho et al, 1989;Ronco et al, 1989), pasando por las diferentes variantes de PCR, los aportes han sido abundantes, no solo en diagnóstico sino en el conocimiento de la enfermedad.…”

Section: Reacción En Cadena De La Polimerasa (Pcr)unclassified

La Peste Negra Del Tomate Y Morrón: Aportes Experimentales Para Su Manejo Integrado

Tozzi¹

2021

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“…Field diagnosis of TSWV infections based on symptoms may mislead as the virus induces a variety of symptoms, often influenced by environment. ELISA, dot-blot hybridization, RT-PCR, immunocapture-RT-PCR (IC-RT-PCR), and real-time fluorescent RT-PCR were applied for the detection of TSWV and other tospoviruses (Bandla et al, 1994;Boonham et al, 2002;Huguenot et al, 1990;Jain, Pappu, Pappu, Culbreath, & Todd, 1998;Resende, de Avila, Goldbach, & Peters, 1991;Rice, German, Mau, & Fujimoto, 1990;Ronco et al, 1989;Weekes, Mumford, Barker, & Wood, 1996).…”

Section: Spotted Wiltmentioning

confidence: 99%