Clonality, outer-membrane proteins profile and efflux pump in KPC- producing Enterobacter sp. in Brazil

Rosa, Juliana Ferraz; Rizek, Camila; Marchi, Ana Paula; Guimarães, Thaís; Miranda, Lourdes; Carrilho, Claudia M.D.M.; Levin, Anna S.; Costa, Sílvia Figueiredo

doi:10.1186/s12866-017-0970-1

Cited by 15 publications

(13 citation statements)

References 24 publications

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“…So far, few studies have reported the effect of efflux pump on carbapenems resistance in Enterobacter spp. [55][56][57] The current study showed that the efflux pump inhibitor CCCP was not able to restore the susceptibility of carbapenem-resistant E. coli, indicating that efflux pump was not involved in the carbapenem resistance in our study.…”

Section: Discussioncontrasting

confidence: 49%

Molecular Mechanisms and Epidemiology of Carbapenem-Resistant Escherichia coli Isolated from Chinese Patients During 2002–2017

Tian¹,

Zheng²,

Sun³

et al. 2020

IDR

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Background: The emergence and spread of carbapenem-resistant Escherichia coli (E. coli) pose a serious threat to human health worldwide. This study aimed to investigate the molecular mechanisms underlying carbapenem resistance and their prevalence among E. coli in China. Methods: A collection of 5796 E. coli clinical isolates were collected from the First Affiliated Hospital of Wenzhou Medical University from 2002 to 2017. Sensitivity to antibiotics was determined using the agar dilution method. The detection of carbapenemases production and the prevalence of resistance-associated genes were investigated through modified carbapenem inactivation method (mCIM), PCR and sequencing. The mutations in outer membrane porins genes (ompC and ompF) were also analyzed by PCR and sequencing assays. The effect of efflux pump mechanism on carbapenem resistance was also tested. E. coli were typed by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Results: A total of 58 strains (1.0%) of carbapenem-resistant E. coli were identified. The strains carrying bla KPC-2 and bla NDM accounted for 22.4% (13/58) and 51.7% (30/58), respectively. Among bla NDM -positive strains, 27 bla NDM genes were assigned to bla NDM-5 , while the remaining three strains were bla NDM-1 , whereas bla VIM , bla IMP , bla OXA-48 , and bla SHV were not found. The CTX-M-type β-lactamase genes accounted for 96.6% (56/58). In addition, bla TEM-1 genes were identified in 58.6% of tested strains. In carbapenem-resistant isolates, mutations in OmpC (the majority of mutated sites were D192G and Q104_F141del, accounting for 54.5%) and OmpF (large deletions S75_V127del, W83_D135del and Q88_D135del) were detected. Of note, the antibiotic resistance was not associated with overexpression of efflux pump. Moreover, MLST categorized the 58 carbapenem-resistant isolates into 19 different sequence types. PFGE analysis revealed that homology among the carbapenem-resistant isolates was low and sporadic. Conclusion: The bla NDM was the principal resistance mechanism of carbapenem-resistant E. coli in the hospital. bla NDM-5 is becoming a new threat to public health and the alteration of outer membrane porins might help further increase the MIC of carbapenem.

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Section: Discussioncontrasting

confidence: 49%

Molecular Mechanisms and Epidemiology of Carbapenem-Resistant Escherichia coli Isolated from Chinese Patients During 2002–2017

Tian¹,

Zheng²,

Sun³

et al. 2020

IDR

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“…In agreement with our survey, in two study from China and Germany 91 and 37.8% of isolates were obtained from RTIs, respectively [10,26]. In contrast, in several investigations blood and abdominal samples were the most frequent sites of Enterobacter isolation [8, 27,28]. In this study, 64.4% of isolates were recovered from ICU ward.…”

Section: Discussionsupporting

confidence: 89%

“…In susceptibility testing, all isolates were resistant to all of the tested antimicrobials, with 93% of strains showing MDR phenotype, making them a major therapeutic treat in our area. Based on CLSI, resistance to one or more carbapenems is considered as carbapenem resistance [27]. Hence, 27.8% our isolates were non-susceptible to imipenem (carbapenem resistant).…”

Section: Discussionmentioning

confidence: 99%

Emergence of OXA-10 and OXA-48 like carbapenemases among Enterobacter isolates from inpatients in southern Iran

Moradi

Khashei

Malekzadegan

et al. 2021

Preprint

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Background The global spread of carbapenemase-producing Enterobacteriaceae represents a public health concern. The aim of this study was to investigate prevalence of carbapenem resistance, oxacillinase types and the presence of class 1–3 integrons among Enterobacter clinical isolates from an Iranian inpatients’ population. Methods Ninety Enterobacter isolates recovered from hospitalized patients were diagnosed by the standard microbiological methods. Antibiogram pattern was also determined. The presence of class 1–3 integrons and four types of oxacillinase genes were assessed using PCR. Results Of the 90 Enterobacter isolates, the most common species was identified as E. aerogenes, (45.6%), followed by E. cloacae (30%). The highest resistance rate was against to ampicillin (96.7%). Multi-drug resistance (MDR) was substantial (93%). Carbapenemase producers were detected in 96% of carbapenem resistant isolates by mCIM test. The frequency of evaluated genes was as follows: intI1 = 50 (55.6%), intI2 = 12 (13.3%), blaoxa−1 =6 (6.7%), blaoxa−2 =5 (5.6%), blaoxa−10 =18 (20%), and blaoxa−48 =18 (20%). Conclusion The determinants of class 1 integron with OXA-10 and OXA-48 like carbapememases have been responsible of relatively considerable of carbapenem resistance among isolates. This is the first OXA-10 and OXA-48-producing Enterobacter spp. in Iran, indicating that the prevalence of oxacillinases might be on the rise in country.

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“…Regarding efflux pumps, the resistance-nodulation-division (RND) group is a major mechanism of multi-drug resistance in Enterobacteriaceae. Among the different efflux systems, the AcrAB-TolC RND system is the most common, and has been identified among carbapenem-resistant E. cloacae [189], E. coli [190], and K. pneumoniae [191]. Alteration of porin synthesis, such as deficiency of OmpK-35 and 36 in K. pneumoniae [192,193], and loss of OmpF and OmpC in E. coli [194], have been described in AmpCand carbapenemase-producing Enterobacteriaceae.…”

Section: Enterobacteriaceaementioning

confidence: 99%

The Current Burden of Carbapenemases: Review of Significant Properties and Dissemination among Gram-Negative Bacteria

2020

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Carbapenemases are β-lactamases belonging to different Ambler classes (A, B, D) and can be encoded by both chromosomal and plasmid-mediated genes. These enzymes represent the most potent β-lactamases, which hydrolyze a broad variety of β-lactams, including carbapenems, cephalosporins, penicillin, and aztreonam. The major issues associated with carbapenemase production are clinical due to compromising the activity of the last resort antibiotics used for treating serious infections, and epidemiological due to their dissemination into various bacteria across almost all geographic regions. Carbapenemase-producing Enterobacteriaceae have received more attention upon their first report in the early 1990s. Currently, there is increased awareness of the impact of nonfermenting bacteria, such as Acinetobacter baumannii and Pseudomonas aeruginosa, as well as other Gram-negative bacteria that are carbapenemase-producers. Outside the scope of clinical importance, carbapenemases are also detected in bacteria from environmental and zoonotic niches, which raises greater concerns over their prevalence, and the need for public health measures to control consequences of their propagation. The aims of the current review are to define and categorize the different families of carbapenemases, and to overview the main lines of their spread across different bacterial groups.

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Clonality, outer-membrane proteins profile and efflux pump in KPC- producing Enterobacter sp. in Brazil

Cited by 15 publications

References 24 publications

<p>Molecular Mechanisms and Epidemiology of Carbapenem-Resistant <em>Escherichia coli</em> Isolated from Chinese Patients During 2002–2017</p>

<p>Molecular Mechanisms and Epidemiology of Carbapenem-Resistant <em>Escherichia coli</em> Isolated from Chinese Patients During 2002–2017</p>

Emergence of OXA-10 and OXA-48 like carbapenemases among Enterobacter isolates from inpatients in southern Iran

The Current Burden of Carbapenemases: Review of Significant Properties and Dissemination among Gram-Negative Bacteria

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