Clonality of Streptococcus pneumoniae Serotype 1 Isolates from Pediatric Patients in the United States

Abstract

We compared Streptococcus pneumoniae serotype 1 isolates causing disease among children in six geographic regions of the United States to determine genetic relatedness. Genomic fingerprints were determined by repetitive element polymorphism PCR (Rep-PCR). Multilocus sequence type characterization was performed on selected isolates. Four different genomic banding patterns were identified by Rep-PCR. One profile (clone 1) was predominant and matched sequence type 227.

Streptococcus pneumoniae serotype 1 causing invasive disease among children in Barcelona over a 20-year period (1989–2008)

“…Nevertheless, in our series, the incidence of serotype 1 was significantly higher in older children than in younger children (<2 years). This observation has also been reported by other authors [14]. The low prevalence of serotype 1 in carriers could also be an explanation for the low rate of antibiotic resistance and the relative stability of clonal composition.…”

Temporal Trends of Invasive Disease Due to Streptococcus pneumoniae among Children in the Intermountain West: Emergence of Nonvaccine Serogroups

“…Children with non-PCV7 infections had hospital stays nearly twice as long as children with IPD due to the PCV7 serogroups, in part because of the high rate of empyema. Other regions of the United States and the United Kingdom are reporting increases among children in the incidence of complicated pneumonia related to serotype 1 disease, specifically pneumonia caused by sequence type 227 [33,[43][44][45][46][47][48]. We believe that surveillance for serotype 1 disease and strategies to prevent its further emergence in the United States will become increasingly important.…”

An Outbreak of Serotype 1Streptococcus pneumoniaeMeningitis in Northern Ghana with Features That Are Characteristic ofNeisseria meningitidisMeningitis Epidemics

“…Individual clonal complexes that belong to the same serotype have different abilities to cause invasive disease [23], suggesting that clonal complex-specific virulence determinants might be important as well. It is not clear whether the virulence of the 3 major subgroups of serotype 1 pneumococci (which have distinct geographic distributions [9,25]) is determined primarily by the capsular serotype-and is, therefore, uniform-or whether lineage-specific genetic differences modulate the potential to cause particular types of invasive disease. Our results suggest that the ST217 clonal complex might have at University of Ulster at Coleraine on March 31, 2015 http://jid.oxfordjournals.org/ Downloaded from a particular propensity to cause meningitis.…”