Clonal structure and 21-year evolution of Streptococcus pneumoniae serotype 1 isolates in northern Spain

Marimón, José María; Ercibengoa, María; Alonso, Marta; Zubizarreta, Marco López; Pérez-Trallero, Emilio

doi:10.1111/j.1469-0691.2009.02883.x

Cited by 11 publications

(8 citation statements)

References 14 publications

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“…In Portugal serotype 1 was the second most important serotype in adult IPD between 2006–2008 [6]. In neighboring Spain, outside the Madrid area where PCV7 was available but not included in the national immunization plan similar to Portugal, an increased importance of serotype 1 in IPD both in the group targeted for PCV7 vaccination as well as in older children and adults was also documented [39]. The decline in the importance of serotype 1 as a cause of IPD with age in adults reported previously [6] was also seen in this period.…”

Section: Discussionmentioning

confidence: 92%

The Majority of Adult Pneumococcal Invasive Infections in Portugal Are Still Potentially Vaccine Preventable in Spite of Significant Declines of Serotypes 1 and 5

Horácio

Diamantino-Miranda²,

Aguiar³

et al. 2013

PLoS ONE

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In Portugal, pneumococcal conjugate vaccines have been administered to children outside of the national immunization plan since 2001. We determined the serotype and antimicrobial susceptibility of 1265 isolates responsible for adult invasive pneumococcal infections (IPD) between 2009 and 2011 and compared the results with previously published data from 1999 to 2008. Serotypes 3 (12.6%), 7F (10.0%), 19A (9.1%), 14 (8.4%), 1 (6.9%) and 8 (6.2%) were the most frequent and together accounted for 53.2% of adult IPD. Serotypes 1 and 5 declined significantly while serotype 34, not included in any vaccine, increased. Taken together, the serotypes included in the 13-valent conjugate vaccine (PCV13) peaked among adult IPD isolates in 2008 (70.2%) and declined since then reaching 53.5% in 2011. The decline in the serotypes included in the 23-valent polysaccharide vaccine since 2007 was also significant but much more modest with 79.2% of the isolates causing IPD in 2011 expressing these serotypes. Since the changes in serotypes causing IPD in adults coincided with the 10-valent and PCV13 introduction in children, it is unlikely that vaccination triggered these changes although it may have accelerated them. The proportion of IPD caused by serotypes included in the 7-valent conjugate vaccine remained stable (19.0%). Both penicillin non-susceptibility and erythromycin resistance increased in the study period, with serotypes 14 and 19A accounting for the majority of resistant isolates.

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Section: Discussionmentioning

confidence: 92%

The Majority of Adult Pneumococcal Invasive Infections in Portugal Are Still Potentially Vaccine Preventable in Spite of Significant Declines of Serotypes 1 and 5

Horácio

Diamantino-Miranda²,

Aguiar³

et al. 2013

PLoS ONE

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“…The MLST306 serotype 1 clone has emerged as being responsible for over 80% of serotype 1 diseases in many parts of the world, and has been associated with invasive pneumococcal disease outbreaks [9,10,12,13]. MLST306 pneumococci express allele 5 PLY which differs in a few amino acids from haemolytic allele 1 PLY and lacks any haemolytic activity but retains the ability to bind to cholesterol-containing membranes [9,21].…”

Section: Discussionmentioning

confidence: 99%

“…In contrast, dominating clones of serotypes 1 and 8 are frequently found in invasive diseases that, however, show lower case-fatality rates [4][5][6][7]. Among serotype 1 pneumococci, the recently emerging MLST306 clone now dominates by over 80% in many parts of the world [8][9][10][11][12][13]. While the degree of encapsulation positively associates with colonization prevalence and virulence [5], other bacterial factors that affect the interaction with the host´s innate immune system might also play a role.…”

Section: Introductionmentioning

confidence: 99%

Serotype 1 and 8 Pneumococci Evade Sensing by Inflammasomes in Human Lung Tissue

et al. 2015

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Streptococcus pneumoniae is a major cause of pneumonia, sepsis and meningitis. The pore-forming toxin pneumolysin is a key virulence factor of S. pneumoniae, which can be sensed by the NLRP3 inflammasome. Among the over 90 serotypes, serotype 1 pneumococci (particularly MLST306) have emerged across the globe as a major cause of invasive disease. The cause for its particularity is, however, incompletely understood. We therefore examined pneumococcal infection in human cells and a human lung organ culture system mimicking infection of the lower respiratory tract. We demonstrate that different pneumococcal serotypes differentially activate inflammasome-dependent IL-1β production in human lung tissue and cells. Whereas serotype 2, 3, 6B, 9N pneumococci expressing fully haemolytic pneumolysins activate NLRP3 inflammasome-dependent responses, serotype 1 and 8 strains expressing non-haemolytic toxins are poor activators of IL-1β production. Accordingly, purified haemolytic pneumolysin but not serotype 1-associated non-haemolytic toxin activates strong IL-1β production in human lungs. Our data suggest that the evasion of inflammasome-dependent innate immune responses by serotype 1 pneumococci might contribute to their ability to cause invasive diseases in humans.

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“…The most prevalent serotype in patients identified by culture is serotype 1, as in other regions of Spain and in Portugal. Marimon et al [19] reported an increase in the number of IPD cases in children caused by serotype 1 in the Basque region of Spain following the introduction of PCV7. Nunes et al [20] reported an emergence of serotype 1 lineage of pneumococci among healthy carriers in Portugal after 2003.…”

Section: Discussionmentioning

confidence: 99%

Serotype 3 is a common serotype causing invasive pneumococcal disease in children less than 5 years old, as identified by real-time PCR

Selva

Ciruela

Esteva

et al. 2011

Eur J Clin Microbiol Infect Dis

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Serotype 3 is one of the most often detected pneumococcal serotypes in adults and it is associated with serious disease. In contrast, the isolation of serotype 3 by bacterial culture is unusual in children with invasive pneumococcal disease (IPD). The purpose of this study was to learn the serotype distribution of IPD, including culture-negative episodes, by using molecular methods in normal sterile samples. We studied all children<5 years of age with IPD admitted to two paediatric hospitals in Catalonia, Spain, from 2007 to 2009. A sequential real-time polymerase chain reaction (PCR) approach was added to routine methods for the detection and serotyping of pneumococcal infection. Among 257 episodes (219 pneumonia, 27 meningitis, six bacteraemia and five others), 33.5% were identified by culture and the rest, 66.5%, were detected exclusively by real-time PCR. The most common serotypes detected by culture were serotypes 1 (26.7%) and 19A (25.6%), and by real-time PCR, serotypes 1 (19.8%) and 3 (18.1%). Theoretical coverage rates by the PCV7, PCV10 and PCV13 vaccines were 10.5, 52.3 and 87.2%, respectively, for those episodes identified by culture, compared to 5.3, 31.6 and 60.2% for those identified only by real-time PCR. Multiplex real-time PCR has been shown to be useful for surveillance studies of IPD. Serotype 3 is underdiagnosed by culture and is important in paediatric IPD.

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Clonal structure and 21-year evolution of Streptococcus pneumoniae serotype 1 isolates in northern Spain

Cited by 11 publications

References 14 publications

The Majority of Adult Pneumococcal Invasive Infections in Portugal Are Still Potentially Vaccine Preventable in Spite of Significant Declines of Serotypes 1 and 5

The Majority of Adult Pneumococcal Invasive Infections in Portugal Are Still Potentially Vaccine Preventable in Spite of Significant Declines of Serotypes 1 and 5

Serotype 1 and 8 Pneumococci Evade Sensing by Inflammasomes in Human Lung Tissue

Serotype 3 is a common serotype causing invasive pneumococcal disease in children less than 5 years old, as identified by real-time PCR

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