Clonal Spread of IMP-1-Producing
            <i>Pseudomonas aeruginosa</i>
            in Two Hospitals in Singapore

Koh, Tse Hsien; Wang, Grace Chee Yeng; Sng, Li-Hwei

doi:10.1128/jcm.42.11.5378-5380.2004

Cited by 18 publications

(17 citation statements)

References 10 publications

(3 reference statements)

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“…This was due entirely to the clonal expansion of only two lineages, ST235 (BURST group 3) and ST357 (BURST group 108) (4). This is similar to the situation that existed in Singapore in 2000, where only two lineages (BURST groups 29 and 44) accounted for the majority of MBL-producing P. aeruginosa (3).…”

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confidence: 61%

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Multilocus Sequence Types of Carbapenem-Resistant Pseudomonas aeruginosa in Singapore Carrying Metallo-β-Lactamase Genes, Including the Novel bla _IMP-26 Gene

et al. 2010

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Nine imipenem-resistant Pseudomonas aeruginosa isolates were found to contain a variety of metallo-␤-lactamase genes, including bla IMP-1 , bla IMP-7 , bla VIM-2 , bla VIM-6 , and the novel bla IMP-26 . Multilocus sequence typing showed a diversity of sequence types. Comparison with isolates from an earlier study showed that the epidemic clones from 2000 have not become established.Carbapenem-resistant Pseudomonas aeruginosa is an increasing problem worldwide. While many underlying mechanisms may account for carbapenem resistance in this species, the possession of metallo-␤-lactamase (MBL) genes is of particular concern because these enzymes are able to hydrolyze all ␤-lactam antimicrobials with the exception of aztreonam. In addition, these genes may be mobilized and transferred between different species of bacteria. We conducted a study in 2008 to investigate if there were any changes in the epidemiology of P. aeruginosa isolates containing MBL genes in our hospital compared to results from an earlier survey carried out in 2000 (3).Of 2,552 nonduplicate P. aeruginosa organisms isolated in 2008, 123 isolates were imipenem resistant. Of these, 11 were positive for MBL production by imipenem-EDTA disk diffusion (5). Nine of these yielded a product by multiplex PCR for MBL genes (2). The individual MBL genes were then amplified and sequenced. The clonal relationship between isolates with MBL genes was determined by pulsed-field gel electrophoresis (PFGE) of chromosomal DNA restricted with SpeI (3). The PFGE band patterns were analyzed with Bionumerics (Applied Maths NV, Sint-Martens-Latem, Belgium), and all strains with more than 85% similarity were considered to belong to the same clone. All strains were further subjected to multilocus sequence typing (MLST) (1). Because it is a nucleic acid sequence-based method, MLST is able to characterize bacterial types in an unambiguous fashion and establish evolutionary relationships between strains better than band-based methods like PFGE. Representative MBL-producing P. aeruginosa isolates from the 2000 survey were also subjected to PFGE and MLST. MLST profiles were submitted to eBURST V3 (http://eburst.mlst.net/) on 10 March 2010. Isolates sharing six out of seven alleles were assigned to the same BURST group and can be considered to belong to the same clonal complex descended from a common founder genotype. The PFGE, MBL gene sequence, and MLST results are summarized in Fig. 1.In our previous study, 21 of 2,094 (1.0%) of all nonduplicate P. aeruginosa isolates in our hospital had MBL genes (3). With the exception of one isolate with bla IMP-7 , all other isolates had bla IMP-1 and belonged to one of two PFGE clones. Isolates belonging to clone A had sequences identical to that of the original bla IMP-1 first reported in Japan. Four representatives of clone A isolated from our hospital in 2000 had sequence type 964 (ST964) by MLST. Isolates belonging to clone B isolated in 2000 had sequences for variant bla IMP-1 (bla IMP-1v ) with four silent mutations. Three represent...

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confidence: 61%

“…In our previous study, 21 of 2,094 (1.0%) of all nonduplicate P. aeruginosa isolates in our hospital had MBL genes (3). With the exception of one isolate with bla IMP-7 , all other isolates had bla IMP-1 and belonged to one of two PFGE clones.…”

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confidence: 99%

Multilocus Sequence Types of Carbapenem-Resistant Pseudomonas aeruginosa in Singapore Carrying Metallo-β-Lactamase Genes, Including the Novel bla _IMP-26 Gene

et al. 2010

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“…16,17 In Southeast Asia, IMP-1 MBL has been detected in carbapenem-resistant P. aeruginosa from Singapore. 18 The occurrence of the bla IMP-1 gene in various countries suggests the possibility of therapeutic failure in patients infected with organisms that produce IMP-1, because this enzyme possesses a broad-spectrum activity profi le that includes activity against carbapenems. 19 Moreover, IMP-1 is notable for its special character, in that it is resistant to β-lactamase inhibitors such as clavulanate, sulbactam, and tazobactam.…”

Section: Discussionmentioning

confidence: 99%

Class 1 integron containing metallo β-lactamase gene blaIMP-1 in carbapenem-resistant Pseudomonas aeruginosa in Thailand

Boonkerd

Pibalpakdi

Tiloklurs

et al. 2009

Journal of Infection and Chemotherapy

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“…In Asia, single bla IMP-7 -positive P. aeruginosa isolates have been reported in Malaysia 8 and Singapore. 9 The patient in our case had developed respiratory insufficiency while he was in Singapore and he had moved to Hiroshima in January 2006. It is speculated that the blaIMP-7-positive strain was accidentally imported in the patient as an inapparent infection, but there is no evidence for this.…”

Section: Shuntaro Kouda · Ryuichi Kuwahara · Masaru Ohara Masanobu Shmentioning

confidence: 94%

First isolation of blaIMP-7 in a Pseudomonas aeruginosa in Japan

Kouda

Kuwahara

Ohara

et al. 2007

Journal of Infection and Chemotherapy

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Clonal Spread of IMP-1-Producing Pseudomonas aeruginosa in Two Hospitals in Singapore

Cited by 18 publications

References 10 publications

Multilocus Sequence Types of Carbapenem-Resistant Pseudomonas aeruginosa in Singapore Carrying Metallo-β-Lactamase Genes, Including the Novel bla _IMP-26 Gene

Multilocus Sequence Types of Carbapenem-Resistant Pseudomonas aeruginosa in Singapore Carrying Metallo-β-Lactamase Genes, Including the Novel bla _IMP-26 Gene

Class 1 integron containing metallo β-lactamase gene blaIMP-1 in carbapenem-resistant Pseudomonas aeruginosa in Thailand

First isolation of blaIMP-7 in a Pseudomonas aeruginosa in Japan

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Clonal Spread of IMP-1-Producing Pseudomonas aeruginosa in Two Hospitals in Singapore

Cited by 18 publications

References 10 publications

Multilocus Sequence Types of Carbapenem-Resistant Pseudomonas aeruginosa in Singapore Carrying Metallo-β-Lactamase Genes, Including the Novel bla IMP-26 Gene

Multilocus Sequence Types of Carbapenem-Resistant Pseudomonas aeruginosa in Singapore Carrying Metallo-β-Lactamase Genes, Including the Novel bla IMP-26 Gene

Class 1 integron containing metallo β-lactamase gene blaIMP-1 in carbapenem-resistant Pseudomonas aeruginosa in Thailand

First isolation of blaIMP-7 in a Pseudomonas aeruginosa in Japan

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Multilocus Sequence Types of Carbapenem-Resistant Pseudomonas aeruginosa in Singapore Carrying Metallo-β-Lactamase Genes, Including the Novel bla _IMP-26 Gene

Multilocus Sequence Types of Carbapenem-Resistant Pseudomonas aeruginosa in Singapore Carrying Metallo-β-Lactamase Genes, Including the Novel bla _IMP-26 Gene