Clonal Relationship between U.S. and French Serotype V Group B Streptococcus Isolates

Thomas-Bories, Isabelle Le; Fitoussi, F.; Mariani‐Kurkdjian, Patricia; Raymond, Josette; Brahimi, N.; Bidet, Philippe; Lefranc, Valerie; Bingen, Édouard

doi:10.1128/jcm.39.12.4526-4528.2001

Cited by 18 publications

(16 citation statements)

References 26 publications

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“…Extension of PFGE analysis to 21 erythromycinsensitive serotype V isolates revealed diversity within the GBS serotype V population as well as one predominant RDP (pattern B) accounting for 48% of all sensitive serotype V isolates (10 of 21). These findings are in good agreement with data published in previous studies (8,14). Even though direct comparative analysis of our isolates with the predominant U.S. or French serotype V GBS strains was not performed in this work, the B pattern obtained from the majority of our isolates seems to be very similar to the pattern published as being predominant in the United States and France.…”

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confidence: 82%

A Serotype V Clone Is Predominant among Erythromycin-Resistant Streptococcus agalactiae Isolates in a Southwestern Region of Germany

et al. 2003

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One hundred ninety-three Streptococcus agalactiae isolates of neonatal origin and 146 isolates from adult women were analyzed for macrolide resistance and investigated for clonality. Among erythromycin-resistant isolates, serotype V turned out to be the most frequent. Comparative pulsed-field gel electrophoresis analysis revealed genetic clustering of resistant strains and predominance of a single clone family within an otherwise heterogeneous serotype V population. [GBS]) is the leading cause of meningitis and early-onset sepsis in neonates (3,21). Penicillin or ampicillin is the drug of choice for prophylaxis and treatment of GBS disease (1, 5). However, erythromycin and clindamycin are the recommended second-line drugs and the first alternative in case of a ␤-lactam allergy. In recent years, increasing macrolide resistance rates have been reported (9,15,16,17,19). Resistance mechanisms include target site modification mediated by erm genes leading to resistance to macrolides-lincosamides-streptogramin B (MLS phenotype) with either inducible or constitutive expression or an efflux mechanism encoded by mef genes mediating resistance only to 14-and 15-membered macrolides (M phenotype). After the first description of serotype V in 1977 (26), several studies reported a major increase of serotype V GBS among clinical isolates in the early 1990s (8,13). To analyze the recent increase in macrolide resistance among GBS isolates in our region, the present study aimed to explore the issue of a clonal spread of resistant strains by means of randomly amplified polymorphic DNA (RAPD) analysis and restriction digest patterns (RDP) derived from pulsed-field gel electrophoresis (PFGE). Moreover, a possible correlation between the increase of a certain serotype and macrolide resistance was explored. Streptococcus agalactiae (group B StreptococcusThe bacterial strains used in this study have been presented previously (20). In brief, of 193 neonatal GBS strains, 26 invasive isolates were cultured from blood or cerebrospinal fluid while 167 isolates were grown from urine samples and swab cultures. A total of 146 GBS isolates from vaginal swabs of pregnant women were randomly collected. Serotyping was performed by using an enzymatic extraction method (2). All erythromycin-resistant isolates were screened by PCR analysis for erythromycin resistance genes (mefA or mefE, ermB, and ermTR) by using primers previously described (7,23,24). PCR assays were reproducibly repeated at least three times for every strain. Differentiation of specific MLS phenotypes was performed by use of a triple-disk test (10). For RAPD analysis, the core sequence of phage M13 and the previously described primer OPS16 were used (6, 12) and visual inspection of bands served to compare RAPD patterns for similarity. Different isolates with the same migration distances of all visible bands were considered highly similar. The presence or absence of two distinct bands was deemed to indicate a difference. Chromosomal DNA of GBS strains was prepared by modifications o...

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confidence: 82%

A Serotype V Clone Is Predominant among Erythromycin-Resistant Streptococcus agalactiae Isolates in a Southwestern Region of Germany

et al. 2003

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“…Genetic diversity among type V isolates has also been observed by Thomas-Bories et al (17), as they found 11 distinct patterns in 64 isolates. In contrast to our V/R1,R4 isolates, the NT/R1,R4 isolates appeared to have retained genetic homogeneity, with three-quarters still classified within PFGE profile 4.…”

Section: Discussionsupporting

confidence: 53%

DNA Macrorestriction Analysis of Nontypeable Group B Streptococcal Isolates: Clonal Evolution of Nontypeable and Type V Isolates

et al. 2005

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Group B streptococci (GBS) are serotyped according to capsular polysaccharide (CPS) type (Ia to VIII); an isolate is classified as nontypeable (NT) if no detectable CPS is found. Surface-localized protein antigens (␣,␤, R1, and R4) serve as additional markers to classify GBS isolates, which is particularly useful since NT isolates often express one or more of these proteins. To compare genetic resemblance among isolates with similar protein profiles, we studied 58 NT isolates digested with the SmaI macrorestriction enzyme prior to pulsed-field gel electrophoresis (PFGE). Of these 58, 15.5% expressed ␣ only, 20.7% expressed ␣؉␤, 15.5% expressed R4, and 25.8% expressed R1,R4, while 22.4% of the isolates expressed no detectable proteins. The largest PFGE profile group, with 48% of the isolates, was group 4, composed primarily of isolates that expressed R1,R4 or no proteins. The second most common profiles were 3 and 32, each with 13.8% of the isolates. Since NT isolates in profile group 4 were highly related to type V isolates, as demonstrated by PFGE profiles, we investigated 45 type V isolates. Two-thirds of the type V isolates within profile group 4 were classified into subgroup 4a, compared to 28.2% of 39 NT isolates. Only 11% of the V/R1,R4 isolates were identical to the prototype group 4 profile, in contrast to 75% of the NT/R1,R4 isolates. A shift of type V isolates into profile 4 subgroups may be indicative of a genetic change over time. PFGE is a valuable approach for comparison of GBS isolate relatedness and for monitoring of NT and typeable GBS isolates for potential clonal divergence.Group B streptococcal (GBS; Streptococcus agalactiae) isolates are classified according to their capsular polysaccharide (CPS) into one of nine types: Ia, Ib, and II to VIII (13,19). However, when tested by routine typing methods, approximately 2.9% of colonizing isolates and 1.4% of invasive isolates lack a detectable CPS type and consequently are categorized as nontypeable (NT) (4, 9, 10).In addition to the CPS type, protein markers can be useful for classification since most GBS isolates express either the c or the R surface-localized protein(s) (9, 10). The c protein is made of two components that are distinguished on the basis of their reaction with trypsin; ␣ is trypsin resistant, while ␤ is trypsin sensitive and binds immunoglobulin A. Isolates may possess only one or both of these components (14). The R proteins (R1, R2, R3, and R4) are the second group of surface proteins and are trypsin resistant; isolates may express one or more of them (10, 18).Identification of surface-localized proteins is an important aspect of the classification of GBS isolates since some proteins are highly associated with specific CPS types. For example, ␣ protein is commonly expressed by the majority of serotype Ia isolates, while more than half of serotype V isolates possess the R1 and R4 proteins (10). Thus, these CPS type-protein profile associations may permit one to compare similarities among NT and typeable isolates on the basis of ...

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“…Similarly, Fasola et al (1993) and Ferrieri et al (1997) obtained more SmaI PFGE patterns than REA with HindIII or HindIII followed by EcoRI and conventional agarose gel electrophoresis. Many researchers who used PFGE analysis of GBS strains have documented considerable genetic diversity among strains of similar serotypes (Fasola et al, 1993;Rolland et al, 1999;Le Thomas-Bories et al, 2001;Moyo et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

“…Of these, PFGE is the most versatile and powerful technique for the study of chromosomal relatedness among bacterial strains as it is reproducible and very discriminative. Many researchers have demonstrated the discriminatory ability and reliability of PFGE in further differentiation of GBS strains of the same serotype (Rolland et al, 1999;Le Thomas-Bories et al, 2001;Benson et al, 2002;Moyo et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

“…Hence molecular approaches which are reproducible and highly discriminatory for differentiating individual strains of bacterial pathogens are often applied to complement serotyping. These approaches include multilocus enzyme analysis (Quentin et al, 1995), ribotyping (Huet et al, 1993;Chatellier et al, 1996), random amplified polymorphic DNA analysis (Chatellier et al, 1997;Limansky et al, 1998;Martinez et al, 2000) and PFGE (Fasola et al, 1993;Gordillo et al, 1993;Rolland et al, 1999;Le Thomas-Bories et al, 2001;Benson et al, 2002;Moyo et al, 2002), analysis of insertion sequences (Rolland et al, 1999;Dmitriev et al, 2004), analysis of mobile genetic elements (Kong et al, 2003) and, more recently, multilocus sequence typing (Jones et al, 2003). Of these, PFGE is the most versatile and powerful technique for the study of chromosomal relatedness among bacterial strains as it is reproducible and very discriminative.…”

Section: Introductionmentioning

confidence: 99%

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Macrorestriction analysis of Streptococcus agalactiae (group B Streptococcus) isolates from Malaysia

Thong

Ling

Kong

et al. 2004

Journal of Medical Microbiology

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Streptococcus agalactiae or group B streptococci (GBS) often colonize the gastrointestinal and urogenital tracts of women, who may transmit these organisms to their offspring during the birth process. Using PFGE analysis, the genetic diversity of GBS was studied for strains isolated from pregnant women and their newborn infants in a teaching hospital. A total of 48 different PFGE profiles were obtained from 123 strains, with one profile (S1) appearing to be predominant among both groups studied. There was good overall correlation between the profiles obtained for strains from mother-infant pairs and for strains isolated from different body sites in the same individual. Occasional discrepancies seen in related body sites and among mother-infant pairs suggest concurrent carriage of different strains in the same individual as well as the possibility of an environmental source of organism for the neonate. The overall results demonstrated that many variants of GBS strains occur in Malaysia.

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Clonal Relationship between U.S. and French Serotype V Group B Streptococcus Isolates

Cited by 18 publications

References 26 publications

A Serotype V Clone Is Predominant among Erythromycin-Resistant Streptococcus agalactiae Isolates in a Southwestern Region of Germany

A Serotype V Clone Is Predominant among Erythromycin-Resistant Streptococcus agalactiae Isolates in a Southwestern Region of Germany

DNA Macrorestriction Analysis of Nontypeable Group B Streptococcal Isolates: Clonal Evolution of Nontypeable and Type V Isolates

Macrorestriction analysis of Streptococcus agalactiae (group B Streptococcus) isolates from Malaysia

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