Clonal relationship between multidrug-resistant <i>Escherichia coli</i> ST69 from poultry and humans in Lusaka, Zambia

Shawa, Misheck; Furuta, Yoshikazu; Paudel, Atmika; Kabunda, O'Brian; Mulenga, Evans; Mubanga, Maron; Kamboyi, Harvey; Zorigt, Tuvshinzaya; Chambaro, Herman M.; Simbotwe, Manyando; Hang’ombe, Bernard M.; Higashi, Hideaki

doi:10.1093/femsle/fnac004

Cited by 13 publications

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“…This correlates with a previous publication that reported potential clonal transmission between the two reservoirs (humans and poultry) in a rural Ghanaian town [30]. Another recent African study, which also correlates with results from the present study, showed a complex relationship between multi-resistant E. coli isolates in poultry and humans indicating that multi-resistance control must involve a One-Health approach and a multi-sectoral collaboration [32]. In our study, the isolation of closely related E. coli ST1722 isolates from humans that were not in close proximity to each other furthermore indicates clonal spread of resistant bacteria in the human community.…”

Section: Discussionsupporting

confidence: 93%

“…Another recent African study, which also correlates with results from the present study, showed a complex relationship between multi-resistant E. coli isolates in poultry and humans indicating that multi-resistance control must involve a One-Health approach and a multi-sectoral collaboration [32]. In our study, the isolation of closely related E.…”

Section: Discussionsupporting

confidence: 90%

“…MLST analysis showed that the six E. coli isolates with serogroup H9 belonged to sequence type (ST)155, three isolates with serogroup O1:H25 belonged to ST1722 and two isolates with ST10 were predicted to have serogroup H27. Six different FimH types (23,24,32,41,54,153) were identified among all the E. coli isolates except one in which it was not detected. Different virulence factors belonging to the four main virulence classes of E. coli pathotypes, namely colonization, fitness, toxins, and effectors, were identified among the E. coli isolates (Table S1).…”

Section: Virulence Factors Of the Recovered Resistant Isolatesmentioning

confidence: 95%

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Genetic characterization of Escherichia coli and Klebsiella spp. from humans and poultry in Nigeria

Jesumirhewe

Rosel

Allerberger

et al. 2023

Access Microbiology

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The emergence of antibiotic resistance in livestock, especially food-producing animals, is of major public health importance as a result of the possibility of these bacteria entering the food chain. In this study, the genetic characteristics of antibiotic-resistant Escherichia coli and Klebsiella spp. isolates from humans and poultry in Edo state, Nigeria, were investigated. In April 2017, 45 Klebsiella spp. and 46 E. coli isolates were obtained from urine, clinical wounds, nasal and chicken faecal samples. Isolates were recovered and identified as previously described. Species identification was achieved by matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry and ribosomal multilocus sequence typing. Antimicrobial susceptibility testing was carried out using the Kirby–Bauer method for 12 antibiotics. A double disc synergy test was used to screen for extended-spectrum beta-lactamse (ESBL) production. Whole genome sequencing was performed for strain characterization of the isolates. Thirteen Klebsiella spp. isolates yielded positive results by the ESBL phenotypic test and harboured ESBL genes. Of the 46 E. coli isolates, 21 human and 13 poultry isolates were resistant to at least one of the tested antibiotics. Four human E. coli isolates harboured ESBL genes and revealed positive results when applying ESBL double disc synergy tests. ESBL genes in the Klebsiella spp. and E. coli isolates include bla CTX-M-15 and bla SHV-28. Whole genome-based core gene multilocus sequence typing of the Klebsiella spp. and E. coli isolates revealed a close relatedness among the isolates. An integrated ‘One Health’ surveillance system is required to monitor transmission of antimicrobial resistance in Nigeria.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 90%

Section: Virulence Factors Of the Recovered Resistant Isolatesmentioning

confidence: 95%

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Genetic characterization of Escherichia coli and Klebsiella spp. from humans and poultry in Nigeria

Jesumirhewe

Rosel

Allerberger

et al. 2023

Access Microbiology

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“…The other STs: ST10, ST69, ST58 and ST1308 have previously been isolated from both humans and livestock (Borges et al, 2019;Day et al, 2019;McKinnon et al, 2018;Seo et al, 2023;Shawa et al, 2021). ST10, ST58, and ST69 are commonly associated with extraintestinal infections in humans (Manges et al, 2019;Riley, 2014).…”

Section: Discussionmentioning

confidence: 99%

The characterisation of antimicrobial resistantEscherichia colifrom dairy calves

Mwenifumbo¹,

Zhao²,

Ahmed³

et al. 2023

Preprint

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Dairy calves, particularly pre-weaned calves have been identified as a common source of multidrug (MDR) resistant E. coli. However, the strains and whether their resistance genes are plasmid or chromosomally located have not been well characterised. Our study examined the phenotype and genotype of antimicrobial resistant E. coli isolated from young calves (≤ 14 days old). Recto-anal swab enrichments from 40 dairy calves located on four dairy farms were examined for tetracycline, streptomycin, ciprofloxacin, and third-generation cephalosporin resistant E. coli. Fifty-eight percent (23/40) of calves harboured antimicrobial resistant E. coli: 18/40 (45%) harboured tetracycline resistant and 25% (10/40) harboured chromosomal mediated AmpC producing E. coli. Whole genome sequencing of 27 isolates revealed five sequence types, with ST88 being the dominant ST (17/27, 63% of the sequenced isolates) followed by ST1308 (3/27, 11%), along with the extraintestinal pathogenic E. coli lineages ST69 (3/27), ST10 (2/27, 7%), and ST58 (1/27, 4%). Additionally, 16 isolates were MDR, harbouring additional resistance genes that were not tested phenotypically. Oxford Nanopore long-read sequencing technologies enabled the location of multiple resistant gene cassettes in IncF plasmids to be determined. A phylogenetic comparison of the ST10 and ST69 isolates demonstrated that the calf derived isolates were distinct from other New Zealand animal, human, and environmental isolates. and highlights the importance of understanding the sources of antimicrobial resistance.

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“…ST131 is the predominant lineage associated with ESBL-associated human urinary tract and blood infections globally [56,57], although other lineages such as ST69 are on the rise [56,58]. Both ST131 and ST69 strains have been isolated from other animals, particularly poultry and dogs, worldwide [27,[59][60][61].…”

Section: Genetic Relatednessmentioning

confidence: 99%

Whole genome sequence analysis of ESBL-producing Escherichia coli recovered from New Zealand freshwater sites

2022

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Extended-spectrum beta lactamase (ESBL)-producing Escherichia coli are often isolated from humans with urinary tract infections and may display a multidrug-resistant phenotype. These pathogens represent a target for a One Health surveillance approach to investigate transmission between humans, animals and the environment. This study examines the multidrug-resistant phenotype and whole genome sequence data of four ESBL-producing E . coli isolated from freshwater in New Zealand. All four isolates were obtained from a catchment with a mixed urban and pastoral farming land-use. Three isolates were sequence type (ST) 131 (CTX-M-27-positive) and the other ST69 (CTX-M-15-positive); a phylogenetic comparison with other locally isolated strains demonstrated a close relationship with New Zealand clinical isolates. Genes associated with resistance to antifolates, tetracyclines, aminoglycosides and macrolides were identified in all four isolates, together with fluoroquinolone resistance in two isolates. The ST69 isolate harboured the bla CTX-M-15 gene on a IncHI2A plasmid, and two of the three ST131 isolates harboured the bla CTX-M-27 genes on IncF plasmids. The last ST131 isolate harboured bla CTX-M-27 on the chromosome in a unique site between gspC and gspD. These data highlight a probable human origin of the isolates with subsequent transmission from urban centres through wastewater to the wider environment.

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Clonal relationship between multidrug-resistant Escherichia coli ST69 from poultry and humans in Lusaka, Zambia

Cited by 13 publications

References 0 publications

Genetic characterization of Escherichia coli and Klebsiella spp. from humans and poultry in Nigeria

Genetic characterization of Escherichia coli and Klebsiella spp. from humans and poultry in Nigeria

The characterisation of antimicrobial resistantEscherichia colifrom dairy calves

Whole genome sequence analysis of ESBL-producing Escherichia coli recovered from New Zealand freshwater sites

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