Clonal Propagation of Vetiveria zizanioides L. through Tissue Culture Technique

Abstract

AbstrakMetode perbanyakan in vitro tanaman akar wangi (Vetiveria zizanioides) secara efektif telah dikembangkan pada penelitian ini. Beberapa variasi media digunakan untuk inisiasi dan multiplikasi tunas. Pembentukan tunas dapat diinisiasi dari eksplan crown yang dikulturkan pada medium MS dengan penambahan zat pengatur tumbuh Benzyladenine (BA) 2 mgL -1 . Sedangkan untuk multiplikasi tunas, konsentrasi BA yang efektif dalam media adalah 3 mgL -1 , dengan rata-rata jumlah tunas yang terbentuk sebesar 126 tunas/eksplan. Penggunaan BA konsentrasi tinggi (3-5 mgL -1 ) pada media multiplikasi dapat memacu terbentuknya lebih banyak tunas, namun tunas yang dihasilkan lebih pendek. Sebaliknya, penggunaan BA dengan konsentrasi rendah (1-2 mgL -1 ) pada media multiplikasi menghasilkan lebih sedikit tunas, tetapi tunas yang dihasilkan lebih panjang. Regenerasi planlet diperoleh dengan menginduksi akar pada tunas pada media MS yang ditambah dengan zat pengatur tumbuh NAA 1 mgL -1 . Tanaman in vitro akar wangi telah berhasil ditumbuhkan pada media tanah di bawah kondisi rumah kaca. Dengan menggunakan metode di atas, maka dimungkinkan perbanyakan bibit akar wangi secara massal melalui teknik kultur jaringan. Kata kunci-Vetiveria zizanioides, in vitro, inisiasi dan multiplikasi tunas, regenerasi plantlet, AbstractIn vitro propagation method for vetiver (Vetiveria zizanioides L.) had been effectively developed in this study. Several variations of media were used for shoot initiation and multiplication. Shoot formation was initiated from crown explant cultured on MS media with the addition of 2 mgL -1 of growth regulator Benzyladenine (BA). Whereas for shoot multiplication, 3 mgL -1 of BA was evidently effective with the average shoot number was 126 shoots per explant. The application of high BA concentration (3-5 mgL -1 ) in multiplication media was capable of inducing more shoots, but the shoots resulted were shorter. In contrast, multiplication media supplemented with low BA concentration (1-2 mgL -1 ) yielded less shoots, but the shoots were longer. Plantlet regeneration was accomplished by inducing roots in the shoots yielded on MS media containing 1 mgL -1 growth regulator NAA. In vitro plants of vetiver had been successfully grown on soil media under greenhouse condition. By using foregoing method, it is possible to conduct mass propagation of vetiver through tissue culture technique.

High frequency plant regeneration of Chrysopogon zizanioides via organogenesis and somatic embryogenesis-an underutilized pharmaceutically valuable biofuel plant

“…Concomitantly, the results ofWidoretno et al (2017) also corroborated the present results that NAA @ 1.0 mg L -1 noted better-rooting e ciency on C. zizanioides. 154 Concomitantly, the results of Widoretno et al (2017) also corroborated the present results that NAA @ 1.0 mg L− 1 noted better-rooting e ciency on C. zizanioides.…”

Effect of Colchicine on In Vitro Growth and Ploidicity of Crown Vetiver Plant (Vetiveria zizanioides L. Nash)

“…Explant crowns were isolated and cultured on MS media supplemented with 2 mg.L -1 BAP. Formed shoots were multiplied on MS media supplemented with 3 mg.L -1 BAP [13]. In vitro shoots were used as the initial explants.…”