Clonal population structure of Pseudomonas avellanae strains of different origin based on multilocus enzyme electrophoresis

Scortichini, M.; Natalini, E.; Angelucci, L.

doi:10.1099/mic.0.26380-0

Cited by 8 publications

(9 citation statements)

References 32 publications

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“…The 22 strains used in this study are listed in Table 1. All of these strains have been previously described by Scortichini et al (2002Scortichini et al ( , 2003. Strains were propagated in the laboratory on nutrient agar (Oxoid) with 5 % sucrose, or King's B medium (King et al, 1954) at 25 uC.…”

Section: Methodsmentioning

confidence: 99%

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Convergent evolution of phytopathogenic pseudomonads onto hazelnut

et al. 2007

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Pseudomonas syringae pv. avellanae (synonym: P. avellanae, Pav) is the causal agent of hazelnut decline in Greece and Italy. The population structure and evolutionary relationships of 22 strains from these two countries were examined by multilocus sequence typing (MLST) of four housekeeping genes (gapA, gltA, gyrB and rpoD). Neighbour-joining and maximum-likelihood phylogenetic analysis revealed that Greek strains isolated from the original 1976 outbreak of hazelnut decline through 1990 were very similar to Italian strains isolated from 2002 through 2004. Other Italian strains that were isolated during the 1990s were very homogeneous and clustered in a clade that was quite distinct from the Greek isolates and Italian isolates from the 2000s. A split decomposition analysis found evidence for recombination between these two highly divergent clades in two of the four MLST housekeeping genes. Incorporating these data into a broad MLST analysis of the P. syringae species complex showed that the Pav Greek and Italian strains from the 2000s clustered with P. syringae phylogroup 1, which is predominantly composed of pathogens of tomato and Brassicaceae hosts, while the Pav Italian strains from the 1990s clustered in P. syringae phylogroup 2 and are most closely related to pea (Pisum sativum L.) pathogens. These results clearly indicate that the ability to infect hazelnuts has arisen twice. This evolutionary process may be due to de novo adaptation to hazelnut by local P. syringae strains (such as the colonizers of Leguminosae crops), or the result of genetic exchange from the original Greek Pav clonal group into a phylogroup 2 strain. The latter explanation is intriguing since there is no exchange of hazelnut propagative material between Italy and Greece, which would be a likely vector for the movement of these pathogens.

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Section: Methodsmentioning

confidence: 99%

“…Multilocus enzyme electrophoresis (MLEE) reveals that Pav is strongly clonal, and shows that the Greek strains are distinct from the Italian strains (Scortichini et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Convergent evolution of phytopathogenic pseudomonads onto hazelnut

et al. 2007

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“…The technique has been used to study epidemiology of several pathogenic bacteria [22-26]. Multilocus restriction typing (MLRT), a recently developed tool, analyses restriction fragment length polymorphism of several housekeeping genes [27-29].…”

Section: Introductionmentioning

confidence: 99%

Genetic relationships between clinical and non-clinical strains of Yersinia enterocolitica biovar 1A as revealed by multilocus enzyme electrophoresis and multilocus restriction typing

Mallik

Virdi

2010

BMC Microbiology

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BackgroundGenetic relationships among 81 strains of Y. enterocolitica biovar 1A isolated from clinical and non-clinical sources were discerned by multilocus enzyme electrophoresis (MLEE) and multilocus restriction typing (MLRT) using six loci each. Such studies may reveal associations between the genotypes of the strains and their sources of isolation.ResultsAll loci were polymorphic and generated 62 electrophoretic types (ETs) and 12 restriction types (RTs). The mean genetic diversity (H) of the strains by MLEE and MLRT was 0.566 and 0.441 respectively. MLEE (DI = 0.98) was more discriminatory and clustered Y. enterocolitica biovar 1A strains into four groups, while MLRT (DI = 0.77) identified two distinct groups. BURST (Based Upon Related Sequence Types) analysis of the MLRT data suggested aquatic serotype O:6,30-6,31 isolates to be the ancestral strains from which, clinical O:6,30-6,31 strains might have originated by host adaptation and genetic change.ConclusionMLEE revealed greater genetic diversity among strains of Y. enterocolitica biovar 1A and clustered strains in four groups, while MLRT grouped the strains into two groups. BURST analysis of MLRT data nevertheless provided newer insights into the probable evolution of clinical strains from aquatic strains.

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“…This protein-based method is used to estimate the overall genetic relatedness among strains by indicating allele variations in chromosomeencoded metabolic housekeeping enzymes [6]. Hence, MLEE is suitable for analyzing the genetic diversity and population structure of bacteria to identify genotypes, clones, antibiotic resistance, enterotoxin production and geographic origin.…”

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Classification and identification of bacteria using mass spectrometry-based proteomics

Dworzański¹,

Snyder²

2005

Expert Review of Proteomics

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Timely classification and identification of bacteria is of vital importance in many areas of public health. Mass spectrometry-based methods provide an attractive alternative to well-established microbiologic procedures. Mass spectrometry methods can be characterized by the relatively high speed of acquiring taxonomically relevant information. Gel-free mass spectrometry proteomics techniques allow for rapid fingerprinting of bacterial proteins using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry or, for high-throughput sequencing of peptides from protease-digested cellular proteins, using mass analysis of fragments from collision-induced dissociation of peptide ions. The latter technique uses database searching of product ion mass spectra. A database contains a comprehensive list of protein sequences translated from protein-encoding open reading frames found in bacterial genomes. The results of such searches allow the assignment of experimental peptide sequences to matching theoretical bacterial proteomes. Phylogenetic profiles of sequenced peptides are then used to create a matrix of sequence-to-bacterium assignments, which are analyzed using numerical taxonomy tools. The results thereof reveal the relatedness between bacteria, and allow the taxonomic position of an investigated strain to be inferred.

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Clonal population structure of Pseudomonas avellanae strains of different origin based on multilocus enzyme electrophoresis

Cited by 8 publications

References 32 publications

Convergent evolution of phytopathogenic pseudomonads onto hazelnut

Convergent evolution of phytopathogenic pseudomonads onto hazelnut

Genetic relationships between clinical and non-clinical strains of Yersinia enterocolitica biovar 1A as revealed by multilocus enzyme electrophoresis and multilocus restriction typing

Classification and identification of bacteria using mass spectrometry-based proteomics

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