Clonal micropropagation of Pistacia lentiscus L. and assessment of genetic stability using IRAP markers

Kılınç, Fatih; Süzerer, Veysel; Çiftçi, Yelda Özden; Onay, Ahmet; Yıldırım, Hakan; Uncuoğlu, Ahu Altınkut; Tilkat, Engin; Koç, İbrahim; Akdemir, Ömer Faruk; Metin, Özge Karakaş

doi:10.1007/s10725-014-9933-9

Cited by 27 publications

(19 citation statements)

References 26 publications

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“…The dendrogram of IRAP data was examined in order to evaluate phylogenetic relations and it was shown that 3 main groups and 1 genotype (LBB) were separated, and retrotransposon mobilities resulting in high polymorphism were affected by the geographical locations of the plant samples. The results of Kılınç et al (2014) and Koç et al (2014) on P. lentiscus based on IRAP and AFLP techniques were supported with the findings of amplification data obtained in our present study in relation to the LTR2 region. Kırdök & Çiftçi (2016) used iPBS, REMAP and IRAP techniques of retrotransposon markers for genetic diversity analysis of Pistacia genus.…”

Section: Discussionsupporting

confidence: 91%

INTRASPECIFIC VARIATIONS STUDIED BY ISSR AND IRAP MARKERS IN MASTIC TREE (Pistacia lentiscus L.) FROM TURKEY

Serttaş¹,

Özcan²

2018

Trakya University Journal of Natural Sciences

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In this study, intra-specific variations in naturally growing and cultivated mastic tree (Pistacia lentiscus L.) samples obtained from western parts of Turkey were examined using ISSR and IRAP marker techniques. Samples from Crete and Chios were also included in the study. Morphological measurements of some leaf characteristics of the samples were performed and the measured data was evaluated statistically with a Pearson Correlation analysis to reveal the correlations between character pairs. ISSR primers produced 81 bands between 161-1884bp with 96.3% polymorphism and IRAP primers produced 72 bands between 124-2027bp with 91.67% polymorphism. Polymorphism information content (PIC) values were 0.458 and 0.418 for ISSR and IRAP, respectively. Genetic similarity matrix was examined with Jaccard's coefficient. Maximum similarity was found between the Cretan samples (LG2 and LG3) with the ISSR analysis (0.933) and between L25A (C1, Bodrum) and L29A (C1, Milas) with the IRAP analysis (0.593). Unweighted pair group method with arithmetic mean (UPGMA) dendrogram was divided into 12 and 4 groups by ISSR and IRAP methods, respectively. Specimens were segregated on 3 main different clusters by the Principal Component Analysis (PCA) based on the combined marker systems. The results showed that P. lentiscus has very high ratios of intraspecific variation. The present work is an original study in terms of large sampling including wild genotypes, cultivated specimen, Chios and Cretan varieties, use of ISSR and IRAP combination, determination of relations between culture and wild genotypes and the use of Bagy-1 retrotransposons in intraspecific polymorphism. This study may be considered as a reference study for studies on gene pools of P. lentiscus and phylogenetic relationships within the species and may contribute to species concept and agricultural breeding programs.Özet: Bu çalışmada, ISSR ve IRAP markör teknikleri kullanılarak Türkiye'nin batı kesiminde doğal olarak yetişen ve kültürü yapılan sakız ağacı (Pistacia lentiscus L.) örneklerindeki tür içi varyasyon analizi yapılmıştır. Girit ve Sakız Adası örnekleri de çalışmaya dahil edilmiştir. Bazı yaprak özelliklerinin morfolojik ölçümleri gerçekleştirilmiş ve aralarındaki korelasyon Pearson Korelasyon analizi ile belirlenmiştir. ISSR primerleri 161-1884bç arasında %96,3 polimorfizm ile 81 bant üretmiştir. IRAP primerleri ise 124-2027bç arasında %91,67 polimorfizm ile 72 bant üretmiştir. Polimorfizm bilgi içeriği (PIC) değerleri 0,458 (ISSR) ve 0,418 (IRAP) arasında bulunmuştur. Genetik benzerlik matriksleri Jaccard katsayısıyla oluşturulmuştur. ISSR sonuçlarında en yüksek benzerlik Girit örnekleri (LG2 ve LG3) arasında (0,933) ve IRAP sonuçlarında ise L25A (C1, Bodrum) ve L29A (C1, Milas) arasında bulunmuştur. UPGMA yöntemiyle kurulan dendrogramlarda sırasıyla ISSR için 12 grup ve IRAP için 4 grup ayrılmıştır. Her iki markör sistemi için ortak kurulan temel bileşen analizi (PCA) grafiğinde 3 farklı küme oluşmuştur. Sonuçlar göstermiştir ki, P. lentiscus yüksek oranlarda tü...

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Section: Discussionsupporting

confidence: 91%

INTRASPECIFIC VARIATIONS STUDIED BY ISSR AND IRAP MARKERS IN MASTIC TREE (Pistacia lentiscus L.) FROM TURKEY

Serttaş¹,

Özcan²

2018

Trakya University Journal of Natural Sciences

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“…The ingredients of culture medium, including mineral nutrients, significantly affect pistachio shoots growth (Akdemir et al, 2012; Kilinç et al, 2015; Marín et al, 2017). Recently, a new medium for pistachio was developed using artificial intelligence tools, even with a poorly sampled experimental design (Nezami-Alanagh et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

“…To avoid those problems, different alternatives have been suggested, based mainly on the combination of macro-, micro-elements and/or vitamins previously proposed for improving the effectiveness of the multiplication of shoots (Onay, 2000; Tilkat et al, 2005, 2008; Benmahioul et al, 2012), or on the incorporation of additional ingredients to the basal culture media, such as calcium gluconate, silver nitrate, ascorbic acid or vanillin, among others (Abousalim and Mantell, 1992, 1994; Parfitt and Almehdi, 1994; Dolcet-Sanjuan and Claveria, 1995; Mederos-Molina and Trujillo, 1999; Akdemir et al, 2012; Kilinç et al, 2015; Marín et al, 2017). …”

Section: Introductionmentioning

confidence: 99%

Combining DOE With Neurofuzzy Logic for Healthy Mineral Nutrition of Pistachio Rootstocks in vitro Culture

et al. 2018

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The aim of this study was to determine the effects of Murashige and Skoog (MS) salts on optimal growth of two pistachio rootstocks, P. vera cv. “Ghazvini” and “UCB1” using design of experiments (DOE) and artificial intelligence (AI) tools. MS medium with 14 macro—and micro-elements was used as base point and its concentration varied from 0 to 5 × MS concentrations. Design of experiments (DOE) software was used to generate a five-dimensional design space by categorizing MS salts into five independent factors (NH4NO3, KNO3, mesos, micros and iron), reducing the experimental design space from 3,125 to just 29 treatments. Typical plant growth parameters such as shoot quality (SQ), proliferation rate (PR), shoot length (SL), and some physiological disorders including shoot-tip necrosis (STN) and callus formation at the base of explants (BC) were evaluated for each treatment. The results were successfully modeled using neurofuzzy logic software. The model delivered new insights, by different sets of “IF–THEN” rules, pinpointing the key role of some ion interactions (SO42- × Cl−, K+ × SO42- × EDTA−, and Fe2+ × Cu2+ × NO3-) for SQ, PR, and SL, whilst physiological disorders (STN and BC) were governed mainly by independent ions as Fe2+ and EDTA−, respectively. In our opinion, the methodology and results obtained in this study is extremely useful to understand the effect of mineral nutrients on pistachio in vitro culture, through discovering new complex interactions among macro—and micro-elements which can be implemented to design new media of plant tissue culture and improve healthy plant micropropagation for any plant species.

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“…The development of reliable protocols for micropropagation of fruit tree scions or rootstocks which are recalcitrant to conventional vegetative propagation, would enable mass production demands to be satisfied. Hence, in vitro propagation has become indispensable for the mass propagation of genetically superior material released in fruit tree genetic improvement programs for the commercialization of new fruit crops cultivars (Akdemir et al 2014;Arab et al 2014aArab et al , b, 2016Arab et al , 2017Arab et al , 2018aBenmahioul et al 2012a;Garoosi et al 2016;Jamshidi et al 2016;Kılınç et al 2015;Marín et al 2016;Nezami-Alanagh et al 2017;Ozden-Tokatli et al 2005;Yıldırım 2012). Propagation of pistachio is extremely difficult, and it is considered recalcitrant to conventional vegetative propagation, even when young plant materials are used in the in vitro culture process (Akdemir et al 2014;Benmahioul et al 2012a;Garoosi et al 2016;Kılınç et al 2015;Marín et al 2016;Nezami-Alanagh et al 2017;Ozden-Tokatli et al 2005;Yıldırım 2012).…”

Section: Micropropagation Without Integrating Callus Phasementioning

confidence: 99%

“…Also, grafting has not been successful to meet the demand of pistachio cultivars grafted onto suitable rootstocks (Marín et al 2016). Therefore, the development of a reliable pistachio micropropagation system is the most commercially-feasible method for mass production of true-to-type plantlets within a short space of time (Akdemir et al 2014;Kılınç et al 2015;Marín et al 2016;Nezami-Alanagh et al 2017). Several micropropagation studies have been carried out on different pistachio species using various plant materials such as explants excised from in vitro grown juvenile seedlings obtained by germinating the seeds and mature plants, different media culture and different plant growth regulators (PGR).…”

Section: Micropropagation Without Integrating Callus Phasementioning

confidence: 99%

Advances in Plant Breeding Strategies: Nut and Beverage Crops

2019

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Clonal micropropagation of Pistacia lentiscus L. and assessment of genetic stability using IRAP markers

Cited by 27 publications

References 26 publications

INTRASPECIFIC VARIATIONS STUDIED BY ISSR AND IRAP MARKERS IN MASTIC TREE (Pistacia lentiscus L.) FROM TURKEY

INTRASPECIFIC VARIATIONS STUDIED BY ISSR AND IRAP MARKERS IN MASTIC TREE (Pistacia lentiscus L.) FROM TURKEY

Combining DOE With Neurofuzzy Logic for Healthy Mineral Nutrition of Pistachio Rootstocks in vitro Culture

Advances in Plant Breeding Strategies: Nut and Beverage Crops

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