Clonal Expansion of Macrolide-Resistant Sequence Type 3<i>Mycoplasma pneumoniae</i>, South Korea

Lee, Joon Kee; Lee, Woo Jung; Lee, Hyunju; Ahn, Young Min; Eun, Byung Wook; Cho, Eun Young; Cho, Hwa Jin; Yun, Ki Wook; Lee, Hoan Jong; Choi, Eun Hwa

doi:10.3201/eid2408.180081

Cited by 31 publications

(43 citation statements)

References 33 publications

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“…Despite the fact that knowledge about the genotype of a clinically relevant strain has had no consequences for therapy up to now, the relations between resistance and typing markers are of epidemiological interest. Regarding the related species M. pneumoniae, results of different studies indicated a relationship between the occurrence of macrolide resistance as well as the clinical severity of infections with particular sequence or VNTR type strains [22][23][24].…”

Section: Discussionmentioning

confidence: 99%

MgpB Types among Mycoplasma genitalium Strains from Men Who Have Sex with Men in Berlin, Germany, 2016–2018

Dumke

Rust²,

Glaunsinger

2019

Pathogens

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Mycoplasma genitalium is a cell wall-less bacterium causing urethritis and other sexually transmitted diseases. Despite a strongly conserved genome, strains in clinical samples can be typed by different methods. To obtain data from the risk population of men having sex with men, we analyzed the typing region in the gene coding for the MgpB adhesin of M. genitalium first in 163 and 45 follow-up samples among patients of two specialized practices in Berlin, Germany. Strains belong to 43 different mgpB types emphasizing the diversity of the genome region. With respect to 133 types previously described, 27 new types were found. However, the majority of strains (64.4%) were assigned to types 4, 6, 113, and 108, respectively. A correlation between mgpB type and the occurrence of mutations associated with macrolide and quinolone resistance was not demonstrated. Investigation of follow-up samples from 35 patients confirmed the same mgpB and, additionally, MG_309 types in 25 cases. In 10 cases, differences between types in subsequent samples indicated an infection with a genetically different strain in the period between samplings. MgpB/MG_309 typing is a useful method to compare M. genitalium strains in samples of individual patients as well as those circulating in different populations

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Section: Discussionmentioning

confidence: 99%

MgpB Types among Mycoplasma genitalium Strains from Men Who Have Sex with Men in Berlin, Germany, 2016–2018

Dumke

Rust²,

Glaunsinger

2019

Pathogens

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“…In conclusion, STs in M. pneumoniae isolates differed by area and year in Japan (1,7,14). Thus, MLST analysis is helpful in understanding worldwide trends among pathogenic M. pneumoniae.…”

Section: Discussionmentioning

confidence: 90%

“…Mycoplasma pneumoniae is a major cause of community-acquired pneumonia, and macrolide-resistant M. pneumoniae is a serious concern in Asia ( 1 – 3 ). Throughout Japan, an outbreak of macrolide-resistant M. pneumoniae infection occurred during 2011–2012 ( 2 ).…”

mentioning

confidence: 99%

Sequence Type Changes Associated with Decreasing Macrolide-Resistant Mycoplasma pneumoniae, Japan

Morozumi¹,

Tajima²,

Sakuma³

et al. 2020

Emerg. Infect. Dis.

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M ycoplasma pneumoniae is a major cause of community-acquired pneumonia, and macrolideresistant M. pneumoniae is a serious concern in Asia (1-3). Throughout Japan, an outbreak of macrolideresistant M. pneumoniae infection occurred during 2011-2012 (2). After this outbreak, the number of drug-resistant strains decreased for every year from 2013 through 2019. In contrast, China and South Korea still showed a high rate of macrolide resistance in M. pneumoniae during 2014-2018 (1,3). We determined antimicrobial drug susceptibility and performed analysis by using multilocus sequence typing (MLST), clonal complexes (CCs), and P1 gene typing for M. pneumoniae isolated from children to identify trends concerning this bacterium in Japan. The Study We obtained nasopharyngeal swab samples from patients who had pneumonia or bronchitis at 21 medical institutions throughout Japan during October 2018-July 2019. We collected samples after obtaining informed consent from patients or their family members (Ethics Committee approval no. 2016-0015,

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“…The comparative whole genome approach was able to define high genetic identity, unique structural diversity, and phylogenetic associations among the 78 M. pneumoniae strains isolated worldwide. pneumoniae pneumonia were defined as previously described (17).…”

Section: Discussionmentioning

confidence: 99%

Comparative Genomics of Mycoplasma pneumoniae isolated from Children with Pneumonia: South Korea, 2010-2016

Lee

Seong

Shin

et al. 2019

Preprint

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Background Mycoplasma pneumoniae is a common cause of respiratory tract infections in children and adults. This study applied high-throughput whole genome sequencing (WGS) technologies to analyze the genomes of 30 M. pneumoniae strains isolated from children with pneumonia in South Korea during the two epidemics from 2010 to 2016 in comparison with a global collection of 48 M. pneumoniae strains which includes seven countries ranging from 1944 to 2017. Results The 30 Korean strains had approximately 40% GC content and ranged from 815,686 to 818,669 base pairs, coding for a total of 809 to 828 genes. Overall, BRIG revealed 99% to > 99% similarity among strains. The genomic similarity dropped to approximately 95% in the P1 type 2 strains when aligned to the reference M129 genome, which corresponded to the region of the p1 gene. MAUVE detected four subtype-specific insertions (three in P1 type 1 and one in P1 type 2), of which were all hypothetical proteins except for one tRNA insertion in all P1 type 1 strains. The phylogenetic associations of 30 strains were generally consistent with the multilocus sequence typing results. eBURST analysis demonstrated two clonal complexes which are accordant with the known P1 typing, with higher diversity among P1 type 2 strains. The phylogenetic tree constructed with 78 genomes including 48 genomes outside Korea formed three clusters, in which the sequence type 3 strains from Korea were divided into two P1 type 1 clusters. Conclusions The comparative genome analysis of the 78 M. pneumoniae strains including 30 strains from Korea by WGS reveals structural diversity and phylogenetic associations, even though the similarity across the strains was very high.

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Clonal Expansion of Macrolide-Resistant Sequence Type 3Mycoplasma pneumoniae, South Korea

Cited by 31 publications

References 33 publications

MgpB Types among Mycoplasma genitalium Strains from Men Who Have Sex with Men in Berlin, Germany, 2016–2018

MgpB Types among Mycoplasma genitalium Strains from Men Who Have Sex with Men in Berlin, Germany, 2016–2018

Sequence Type Changes Associated with Decreasing Macrolide-Resistant Mycoplasma pneumoniae, Japan

Comparative Genomics of Mycoplasma pneumoniae isolated from Children with Pneumonia: South Korea, 2010-2016

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