Clonal diversity of Acinetobacter baumannii clinical isolates in Myanmar: identification of novel ST1407 harbouring blaNDM-1

Aung, Meiji Soe; Hlaing, Myat Su; San, Nilar; Aung, Myint Thazin; Mar, Tin Tin; Kobayashi, Nobumichi

doi:10.1016/j.nmni.2021.100847

Cited by 4 publications

(1 citation statement)

References 11 publications

(10 reference statements)

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“…The majority of the CR-Ab were ST16, ST149 and ST25, which have increasingly been found since 2018 in addition to ST2 [ 5 ], indicating multiclonal dissemination in small animals. The predominant ST16 and ST149 were previously isolated from medical settings in various countries, including Thailand [ 2 , 6 , 9 , 23 , 33 , 34 , 35 ]. In Thailand’s healthcare settings, CR-Ab ST16 has been reported as a minor lineage with approximately 2.96% [ 17 ].…”

Section: Discussionmentioning

confidence: 99%

Emergence and multi-lineages of carbapenemase-producing <i>Acinetobacter baumannii-calcoaceticus</i> complex from canine and feline origins

Leelapsawas

Yindee

Nittayasut

et al. 2022

The Journal of Veterinary Medical Science

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The carbapenemase-producing Acinetobacter baumannii is an important opportunistic bacterium and frequently causes hospital-acquired infections in humans. It also has increasingly been reported in veterinary medicine. This study illustrates multiple clones of carbapenemase-producing A. baumannii disseminating and causing diseases in dogs and cats in Thailand. Between 2016 and 2020, 44 A. baumannii and two A. pittii isolates exhibiting imipenem resistance (MIC ≥ 16 μg/mL) from diagnostic samples were characterized by Pasteur multilocus sequence typing (MLST), sequence grouping (SG), repetitive extragenic palindromic element (rep)-PCR fingerprint analysis and antimicrobial resistance (AMR) profiling. All isolates contained blaOXA-23 in the Tn2006 family, and A. baumannii showed the sequence type (ST) 16 (14/44), ST149 (12/44), ST25 (6/44), ST2 (4/44), ST 1581 (3/44), ST23 (2/44), ST1575 (1/44) and ST1576 (1/44). DNA fingerprint analysis and SG illustrated clonal relationships in the STs and its single locus variants, and AMR gene profiles, including tetracycline and aminoglycoside resistance genes, showed minor variations in the clones. The findings suggest that blaOXA-23 has been spread in multiple clones of A. baumannii and A. pittii from canine and feline hosts. With the collection of multiple AMR genes and intrinsic resistance, antimicrobial options are limited for treatment, and pets can be a potential reservoir of extensively drug-resistant, carbapenemase-producing A. baumannii in the community. Epidemiological tracking by passive and active surveillance in animals, veterinary personnel and hospital environment and preventive measurements should be promoted to decrease the risk of infection and transmission to humans.

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Section: Discussionmentioning

confidence: 99%

Emergence and multi-lineages of carbapenemase-producing <i>Acinetobacter baumannii-calcoaceticus</i> complex from canine and feline origins

Leelapsawas

Yindee

Nittayasut

et al. 2022

The Journal of Veterinary Medical Science

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Role of transcriptomic and genomic analyses in improving the comprehension of cefiderocol activity in Acinetobacter baumannii

Stracquadanio,

Nicolosi,

Privitera

et al. 2024

mSphere

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The mechanisms of action and resistance of cefiderocol (FDC) in Acinetobacter baumannii are still not fully elucidated, but iron transport systems have been evoked in its entry into the cell to reach the penicillin-binding proteins (PBPs). To capture the dynamics of gene expression related to FDC action in various conditions, we report on the genomic and transcriptomic features of seven A. baumannii strains with different FDC susceptibility, focusing on the variants in genes associated with β-lactam resistance and the expression of the siderophore biosynthesis and transport systems acinetobactin and baumannoferrin. We also investigated the expression of the TonB energy transduction system (ETS) and siderophore receptors piu A and pir A. The four clinical samples belonged to the same clonal complex (CC2), and the two strains with the highest FDC MICs showed peculiar variants in PBP2 and amp C. Similarly, the two clinical strains with the lowest MICs shared variants in an outer membrane protein as well as amp C. Gene expression analyses highlighted the up-regulation of the acinetobactin and baumannoferrin genes in response to iron depletion and a down-regulation in the presence of high iron concentrations. In response to FDC, gene expression seemed strain-dependent, probably due to the different metabolic features of each strain. Overall, FDC activates the ETS, confirming the active import of the drug; baumannoferrin, more than acinetobactin, appeared stimulated by FDC in an iron-depleted medium. In conclusion, iron transport systems play a clear role in the FDC uptake, and their expression likely contributes to MIC variation together with β-lactam resistance determinants. IMPORTANCE Acinetobacter baumannii poses a threat to healthcare due to its ability to give difficult-to-treat infections as a consequence of our shortage of antibiotic molecules active on this multidrug-resistant bacterium. Cefiderocol (FDC) represents one of the few drugs active on A. baumannii, and to preserve its activity, this study explored the transcriptomic and genomic features of seven strains with varying susceptibility to FDC. Transcriptomic analyses revealed the different effects of FDC on iron transport systems, promoting mainly baumannoferrin expression—thus more likely related to FDC entry—and the energy transduction systems. These findings suggest that not all iron transport systems are equally involved in FDC entry into A. baumannii cells. Finally, mutations in PBPs and β-lactamases may contribute to the resistance onset. Overall, the study sheds light on the importance of iron availability and metabolic differences in FDC resistance, offering insights into understanding the evolution of resistance in A. baumannii strains.

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Whole - Genome Sequence Analysis of Carbapenem Resistant Acinetobacter baumannii isolated from Medan, North Sumatera, Indonesia

Wiranto,

Soebandrio,

Paramita

et al. 2023

Preprint

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Background Acinetobacter baumannii, an opportunistic pathogen that is responsible for nosocomial infections globally, presents a formidable challenge owing to its ability to develop resistance against antibiotics. This resistance, which includes resistance to carbapenems, the last-resort antibiotics, has led to the emergence of Carbapenem-Resistant Acinetobacter baumannii (CRAB) strains. The distribution of these strains encompasses Indonesia, particularly in Medan. Investigation on its resistance can only be performed thoroughly using Whole Genome Sequencing (WGS) process. Methods The samples used in this study is 32 bacterial isolates from Murni Teguh Memorial Hospital in Medan, North Sumatra, collected between July – December 2022 and has been confirmed as A. baumannii using VITEK-2 system. Bacterial isolates are tested for carbapenem sensitivity using disc diffusion method. DNA isolation is performed using QIAamp DNA mini kit and ran through WGS process using Novaseq 6000 Illumina. Bioinformatics analysis is performed consisting of MLST, phylogenetic tree, identification of elements such as resistance genes, plasmids, insertion sequences, prophage sequences, and CRISPR/Cas system sequences. Results 58 resistance genes and several mutations were identified. The majority of these genes exert their influence on resistance through efflux pump mechanisms. All the carbapenem-resistant isolates showing co-occurrence of blaOXA-23 and blaOXA-51 family genes. MLST analysis revealed that out of the 30 isolates, 27 were identified as A. baumannii and were further divided into 7 distinct Sequence Types (STs), with the majority belonging to ST 2 and ST 1. Furthermore, the analysis uncovered the presence of novel STs, specifically assigned as ST-Past 2456 and ST-Oxf 3213. Aside from resistance genes, 20 plasmids, 89 insertion sequences, 20 prophage sequences, and 1 CRISPR/Cas system identified in this research. Conclusion WGS analysis of A. baumannii isolates from Medan reveals the presence of several resistance determinants that can contribute to the high resistance levels observed in these isolates. Comparative analysis with global isolates indicates that the majority of isolates belong to globally recognized lineages with high resistance levels. Along with the discovery of the new ST in this study, the information obtained is expected to assist other researchers in further studies related to A. baumannii and its resistance to antimicrobials.

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Clonal diversity of Acinetobacter baumannii clinical isolates in Myanmar: identification of novel ST1407 harbouring blaNDM-1

Cited by 4 publications

References 11 publications

Emergence and multi-lineages of carbapenemase-producing <i>Acinetobacter baumannii-calcoaceticus</i> complex from canine and feline origins

Emergence and multi-lineages of carbapenemase-producing <i>Acinetobacter baumannii-calcoaceticus</i> complex from canine and feline origins

Role of transcriptomic and genomic analyses in improving the comprehension of cefiderocol activity in Acinetobacter baumannii

Whole - Genome Sequence Analysis of Carbapenem Resistant Acinetobacter baumannii isolated from Medan, North Sumatera, Indonesia

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