Clonal diversity and epidemiological characteristics of Staphylococcus aureus: high prevalence of oxacillin-susceptible mecA-positive Staphylococcus aureus (OS-MRSA) associated with clinical isolates in Brazil

Andrade-Figueiredo, Mariana; Leal-Balbino, Tereza Cristina

doi:10.1186/s12866-016-0733-4

Cited by 45 publications

(31 citation statements)

References 39 publications

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“…Likewise, ST59-t437-SCCmecV T was the most prevalent OS-MRSA clone in a study from Taiwan [42]. However, this finding differs from other reports; ST88 and ST8 were the most prevalent OS-MRSA clones in Africa [43] and OS-MRSA clones were highly diverse in Brazil [44], potentially due to geographic differences in genetic background. OS-MRSA strains may be misidentified as MSSA by traditional susceptibility testing thereby complicating the diagnosis and appropriate treatment of S. aureus infections.…”

Section: Colonizing or Infectious (No)contrasting

confidence: 62%

Epidemiology of Staphylococcus aureus in neonates on admission to a Chinese neonatal intensive care unit

Geng

et al. 2020

PLoS ONE

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Little is known about the molecular epidemiology of Staphylococcus aureus in Chinese neonatal intensive care units (NICUs). We describe the molecular epidemiology of S. aureus isolated from neonates on admission to Beijing Children's Hospital. Methods From May 2015-March 2016, nasal swabs were obtained on admission from 536 neonates. Cultures were also obtained from body sites with suspected infections. S. aureus isolates were characterized by staphylococcal chromosomal cassette (SCCmec) type, staphylococcal protein A (spa) type, multilocus sequence type (MLST), sasX gene, antimicrobial susceptibility and cytotoxicity. Logistic regression assessed risk factors for colonization. Results Overall, 92 (17%) infants were colonized with S. aureus and 20 (3.7%) were diagnosed with culture-positive S. aureus infection. Of the colonized infants, 70% (64/92) harbored methicillin-susceptible S. aureus (MSSA), 30% (28/92) harbored methicillin-resistant S. aureus (MRSA) while 70% (14/20) of infected infants were culture-positive for MRSA, 30% (6/20) were culture-positive for MSSA. Risk factors for colonization included female sex, age 7-28 days, higher birthweight (3270 IQR [2020-3655] grams) and vaginal delivery (p<0.05). The most common MRSA and MSSA clones were community-associated ST59-SCCmecIVa-t437 (60%) and ST188-t189 (15%), respectively. The sasX gene was not detected. Some MSSA isolates (16%) were penicillin-susceptible and some MRSA isolates (18%) were oxacillin-susceptible. MRSA and MSSA had similar cytotoxicity, but colonizing strains were less cytotoxic than strains associated with infections.

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Section: Colonizing or Infectious (No)contrasting

confidence: 62%

Epidemiology of Staphylococcus aureus in neonates on admission to a Chinese neonatal intensive care unit

Geng

et al. 2020

PLoS ONE

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“…This lineage has also been observed in blood samples in Brazil, highlighting that one of these reports describes two isolates of S. aureus resistant to daptomycin 27 . In Brazil, to the best of our knowledge, there are few reports of USA600/ST45-MRSA-IV isolates, which were collected from skin infection case and from catheter 23, 28…”

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confidence: 80%

Molecular characterization of methicillin-resistant Staphylococcus aureus isolated from blood in Rio de Janeiro displaying susceptibility profiles to non-β-lactam antibiotics

Zuma

Lima

Carvalho-Assef

et al. 2017

Brazilian Journal of Microbiology

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The distinction between healthcare-associated MRSA (HA-MRSA) and community-associated MRSA (CA-MRSA) infections has become increasingly blurred. We assessed the molecular characterization and antimicrobial resistance profile for MRSA isolates from blood. Most of all (81.9%) isolates are related to known HA-MRSA and CA-MRSA epidemic lineages, such as, USA300, USA400, USA600, USA800 and USA1100. This is the first multicenter study in Rio de Janeiro.

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“…[37] Likewise, ST59-t437-SCCmecV T was the most prevalent OS-MRSA clone in a study from Taiwan. [38] However, this finding differs from other reports; ST88 and ST8 were the most prevalent OS-MRSA clones in Africa[39] and OS-MRSA clones were highly diverse in Brazil,[40] potentially due to geographic differences in genetic background. OS-MRSA strains may be misidentified as MSSA by traditional susceptibility testing or as MRSA by molecular detection of mecA , thereby complicating the diagnosis and appropriate treatment of S. aureus infections.…”

Section: Discussionmentioning

confidence: 56%

Epidemiology ofStaphylococcus aureusin Neonates on Admission to a Chinese Neonatal Intensive Care Unit

Geng

et al. 2019

Preprint

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25Purpose: Little is known about the molecular epidemiology of Staphylococcus aureus in 26 Chinese neonatal intensive care units (NICUs). We describe the molecular epidemiology of S. 27 aureus isolated from neonates on admission to Beijing Children's Hospital. 28 29 Methods: From May 2015-March 2016, nasal swabs were obtained on admission from 536 30 neonates. Cultures were also obtained from body sites with suspected infections. S. aureus 31 isolates were characterized by staphylococcal chromosomal cassette (SCCmec) type, 32 staphylococcal protein A (spa) type, multilocus sequence type (MLST), sasX gene, antimicrobial 33 susceptibility and cytotoxicity. Logistic regression assessed risk factors for colonization. 34 35Results: Overall, 92 (18%) infants were colonized with S. aureus and 23 (4%) were diagnosed 36 with culture-positive S. aureus infection. Of the colonized infants, 72% harbored MSSA, while 37 74% of infected infants were culture-positive for MRSA. Risk factors for colonization included 38 female sex, age 7-28 days, birthweight and vaginal delivery. The most common MRSA and 39 MSSA clones were community-associated ST59-SCCmecIVa-t437 (60%) and ST188-t189 40 (15%), respectively. The sasX gene was not detected. Some MSSA isolates (16%) were 41 penicillin-susceptible and some MRSA isolates (18%) were oxacillin-susceptible. MRSA and 42 MSSA had similar cytotoxicity, but colonizing strains were less cytotoxic than strains associated 43 with infections. 44 45 Conclusions: S. aureus colonization was common in infants admitted to our NICU and two 46 community-associated clones predominated. Several non-modifiable risk factors for S. aureus 50 51 Key words: Staphylococcus aureus, neonatal intensive care unit, colonization, MRSA, MSSA 52 4 53 54 Staphylococcus aureus infections represent a significant clinical burden for infants worldwide 55 and were recently found to be the second most common cause of late-onset sepsis in very-low 56 birth weight (VLBW) infants admitted to neonatal intensive care units (NICU) in the United 57 States and United Kingdom.[1, 2] Preterm infants are also at high risk for S. aureus 58 colonization[3], a potential risk factor for subsequent infection. In a recent meta-analysis 59 involving patients admitted to NICUs and ICUs, methicillin-resistant S. aureus (MRSA) 60 colonization was associated with a 24.2 times increased MRSA infection risk.[4] Endemic 61 transmission and outbreaks due to MRSA and methicillin-susceptible S. aureus (MSSA) occur 62 frequently in NICUs.[5] Studying the molecular epidemiology and virulence factors of S. aureus 63 in the NICU population can promote an increased understanding of pathogenesis and ultimately 64 guide preventive strategies. 65 66 While the molecular characteristics of and risk factors for S. aureus colonization and infection 67 have been described for NICU populations across the globe and have increased our knowledge of 68 the global burden,[3, 6] no previous reports have described the molecular characteristics of S. 69 aureus strains...

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Clonal diversity and epidemiological characteristics of Staphylococcus aureus: high prevalence of oxacillin-susceptible mecA-positive Staphylococcus aureus (OS-MRSA) associated with clinical isolates in Brazil

Cited by 45 publications

References 39 publications

Epidemiology of Staphylococcus aureus in neonates on admission to a Chinese neonatal intensive care unit

Epidemiology of Staphylococcus aureus in neonates on admission to a Chinese neonatal intensive care unit

Molecular characterization of methicillin-resistant Staphylococcus aureus isolated from blood in Rio de Janeiro displaying susceptibility profiles to non-β-lactam antibiotics

Epidemiology ofStaphylococcus aureusin Neonates on Admission to a Chinese Neonatal Intensive Care Unit

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