Clonal distribution of pneumococcal serotype 19F isolates from Ghana

Sparding, Nadja; Dayie, Nicholas T. K. D.; Mills, Richael Odarkor; Newman, Mercy J.; Dalsgaard, Anders; Frimodt‐Møller, Niels; Slotved, Hans Christian

doi:10.1016/j.meegid.2015.01.016

Cited by 3 publications

(3 citation statements)

References 30 publications

(11 reference statements)

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“…However, since we did not compare in detail the cps of the serotype 23F with that of the 19F included in ST802, we can only infer from the data the possible exchange of cps loci within this clonal complex [49]. ST802 was previously identified among serotype 19F and 23F pneumococcal strains in Ghana during the pre-vaccination era [9,15,17]. ST802 was previously reported from other countries [24].…”

Section: Discussionmentioning

confidence: 99%

“…The only serotype 23F, which did not cluster into ST802, belonged to ST2174. ST2174 is a double locus variant (DLV) of ST9090, a major clone associated with serotype 19F described in Ghana during the pre-vaccination era [17].…”

Section: Discussionmentioning

confidence: 99%

“…With the increasing trend in MDR among pneumococcal strains globally, the pneumococcal molecular epidemiology network (PMEN) and the PubMLST databases provide a platform for comparison of multilocus sequence type (MLST) strains with other globally disseminated MDR strains. Previous studies in Ghana have identified the proliferation and dissemination of lineages of ST802, ST9090, ST4194, and the hypervirulent ST217 among both carriage and invasive pneumococcal isolates [9,[15][16][17].…”

Section: Introductionmentioning

confidence: 99%

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Molecular Epidemiology of Multidrug-Resistant Pneumococci among Ghanaian Children under Five Years Post PCV13 Using MLST

et al. 2022

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Antibiotic resistance in pneumococci contributes to the high pneumococcal deaths in children. We assessed the molecular characteristics of multidrug-resistant (MDR) pneumococci isolated from healthy vaccinated children under five years of age in Cape Coast, Ghana. A total of 43 MDR isolates were selected from 151 pneumococcal strains obtained from nasopharyngeal carriage. All isolates were previously serotyped by multiplex PCR and Quellung reaction. Susceptibility testing was performed using either the E-test or disk diffusion method. Virulence and antibiotic resistance genes were identified by PCR. Molecular epidemiology was analyzed using multilocus sequence typing (MLST). Vaccine-serotypes 23F and 19F were predominant. The lytA and pavB virulence genes were present in all isolates, whiles 14–86% of the isolates carried pilus-islets 1 and 2, pcpA, and psrP genes. Penicillin, tetracycline, and cotrimoxazole resistance were evident in >90% of the isolates. The ermB, mefA, and tetM genes were detected in (n = 7, 16.3%), (n = 4, 9.3%) and (n = 43, 100%) of the isolates, respectively. However, >60% showed alteration in the pbp2b gene. MLST revealed five novel and six known sequence types (STs). ST156 (Spain9V-3) and ST802 were identified as international antibiotic-resistant clones. The emergence of international-MDR clones in Ghana requires continuous monitoring of the pneumococcus through a robust surveillance system.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular Epidemiology of Multidrug-Resistant Pneumococci among Ghanaian Children under Five Years Post PCV13 Using MLST

et al. 2022

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Re-emergence of pneumococcal colonization by vaccine serotype 19F in persons aged ≥5 years after 13-valent pneumococcal conjugate vaccine introduction—Alaska, 2008–2013

Gounder

Bruden

Rudolph

et al. 2018

Vaccine

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Etiologic Profile of the Pneumococcus in Ghana: A Systematic Review

Arhin,

Donkor,

Slotved

et al. 2024

BioMed Research International

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Objective: To describe the profile of Streptococcus pneumoniae, identify research gaps, and provide in‐depth insights into various aspects related to the pathogen.Methods: Google Scholar, PubMed, and ScienceDirect were searched for all studies on the pneumococcus in Ghana that reported on specimen collected, population and sample size, carriage prevalence, incidence of pneumococcal diseases, age of the study population, types of test performed, serotypes identified, antimicrobial susceptibilities, or molecular analysis on the pneumococci for data extraction.Results: Overall, a total of 7954 results were obtained from the three‐database search, and of this, 24 articles were selected after screening. A total of 924 isolates were accounted for by serotyping/serogrouping. The prevalence of pneumococcal carriage in Ghana ranges from 11.0% to 51.4% in the population depending on the age (≤ 24–80 years), sickle cell disease (SCD), human immunodeficiency virus (HIV) status, or health of the study population, and penicillin (Pen)‐nonsusceptible isolates ranged from 17% to 63%. The prevalence of pneumococci found as the etiologic agent of diseases among Ghanaians ranges from 3.4% for otitis media to 77.7% for meningitis. Overall, the 13‐valent pneumococcal conjugate vaccine (PCV) (PCV‐13) carriage serotypes accounted for 28.4% of the reported pneumococcal isolates. PCV‐13 invasive serotypes accounted for 22.4% of the reported isolates. The non‐PCV‐13 carriage serotypes accounted for most (43.9%) of the reported isolates. In the pre‐PCV‐13 era, the nontypeable (NT) (5.5%) and other nonvaccine types (NVTs) (6.4%) were reported as being predominant. The non‐PCV‐13 serotypes accounted for 4.4% of the reported isolates in invasive pneumococcal disease (IPD) cases. Multidrug resistance (MDR) ranged from 7.8% to 100%.Conclusion: Predicting the invasiveness of pneumococci using molecular typing is the way to go in the future as this will provide answers to the extent to which capsular switching is contributing to the pneumococcal disease burden in Ghana almost a decade after introducing PCV‐13. Continuous monitoring of antibiotic resistance patterns at both phenotypic and genotypic levels, along with serotyping and molecular typing, should be a standard practice in the surveillance of pneumococcal disease burden in Ghana.

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Clonal distribution of pneumococcal serotype 19F isolates from Ghana

Cited by 3 publications

References 30 publications

Molecular Epidemiology of Multidrug-Resistant Pneumococci among Ghanaian Children under Five Years Post PCV13 Using MLST

Molecular Epidemiology of Multidrug-Resistant Pneumococci among Ghanaian Children under Five Years Post PCV13 Using MLST

Re-emergence of pneumococcal colonization by vaccine serotype 19F in persons aged ≥5 years after 13-valent pneumococcal conjugate vaccine introduction—Alaska, 2008–2013

Etiologic Profile of the Pneumococcus in Ghana: A Systematic Review

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