Clonal dissemination of two MRSA strains in Germany

Witte, Wolfgang; Cuny, Cécile; Braulke, C; Heuck, D

doi:10.1017/s0950268800051475

Cited by 28 publications

(14 citation statements)

References 20 publications

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“…Since then, investigators in many other countries have reported the occurrence of the predominant spread of 1 strain in a defined geographic area (Germany [27,28], Poland [16], Turkey [29], Portugal and Spain [18,30,31], Holland [32], and Australia [33]). …”

Section: Discussionmentioning

confidence: 99%

Unusual Spread of a Penicillin-Susceptible Methicillin-Resistant Staphylococcus aureus Clone in a Geographic Area of Low Incidence

Blanc¹,

Petignat²,

Moreillon³

et al. 1999

Clinical Infectious Diseases

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We describe the unusual spread of a penicillin-susceptible methicillin-resistant Staphylococcus aureus (MRSA) clone in hospitals in western Switzerland, where the incidence of MRSA is usually low. During a 2-year period, this clone had been responsible for several outbreaks and had been isolated from >156 persons in 21 institutions. Molecular typing by pulsed-field gel electrophoresis (PFGE) demonstrated that all of these isolates belonged to the same clone. In 1 of the outbreaks, involving 30 cases, the clone was responsible for at least 17 secondary cases. In contrast, during the period of the latter outbreak, 9 other patients harboring different MRSA strains, as assessed by PFGE, were hospitalized in the same wards, but no secondary cases occurred. These observations suggest that this clone, compared with other MRSA strains, had some intrinsic factor(s) that contributed to its ability to disseminate and could thus be considered epidemic.

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Section: Discussionmentioning

confidence: 99%

Unusual Spread of a Penicillin-Susceptible Methicillin-Resistant Staphylococcus aureus Clone in a Geographic Area of Low Incidence

Blanc¹,

Petignat²,

Moreillon³

et al. 1999

Clinical Infectious Diseases

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“…The introduction of SmaI macrorestriction patterns at the beginning of the 1990s led to identification of epidemic strains at national and international level (Witte et al, 1994Criso´stomo et al, 2001).…”

Section: Understanding Evolution Emergence and Spread Of Mrsa By Molmentioning

confidence: 99%

Emergence and spread of antibiotic-resistant Gram-positive bacterial pathogens

Witte

Cuny

Klare

et al. 2008

International Journal of Medical Microbiology

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“…As test organisms for antimicrobial activity assays we used the Gram-negative bacteria Escherichia coli ATCC 25922, E. coli DC2 (antibiotic-susceptible penetration mutant), [37] Pseudomonas aeruginosa ATCC 9027, P. aeruginosa K799/61 (antibiotic-susceptible penetration mutant), [38] Burkholderia cepacia DSM 7288 (resistant to cationic peptides), [39] B. vietnamiensis DSM 11319, B. multivorans BF7 (clinical isolate obtained from Prof. Dr. A. Bauernfeind, MICOER-Institute, Munich, Germany) and the Gram-positive bacteria Staphylococcus aureus SG511, S. aureus 134/ 93 (MRSA), [40] Bacillus subtilis ATCC 6633, Micrococcus luteus ATCC 10240 and ATCC 9341, Mycobacterium vaccae IMET 10670, and Mycobacterium fortuitum B.…”

Section: Antimicrobial Inhibition Assaysmentioning

confidence: 99%

4‐Fluorophenylglycine as a Label for ¹⁹F NMR Structure Analysis of Membrane‐Associated Peptides

et al. 2003

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The non-natural amino acid 4-fluorophenylglycine (4F-Phg) was incorporated into several representative membrane-associated peptides for dual purpose. The (19)F-substituted ring is directly attached to the peptide backbone, so it not only provides a well-defined label for highly sensitive (19)F NMR studies but, in addition, the D and L enantiomers of the stiff side chain may serve as reporter groups on the transient peptide conformation during the biological function. Besides peptide synthesis, which is accompanied by racemisation of 4F-Phg, we also describe separation of the epimers by HPLC and removal of trifluoroacetic acid. As a first example, 18 different analogues of the fusogenic peptide "B18" were prepared and tested for induction of vesicle fusion; the results confirmed that hydrophobic sites tolerated 4F-Phg labelling. Similar fusion activities within each pair of epimers suggest that the peptide is less structured in the fusogenic transition state than in the helical ground state. In a second example, five doubly labelled analogues of the antimicrobial peptide gramicidin S were compared by using bacterial growth inhibition assays. This cyclic beta-sheet peptide could accommodate both L and D substituents on its hydrophobic face. As a third example, we tested six analogues of the antimicrobial peptide PGLa. The presence of d-4F-Phg reduced the biological activity of the peptide by interfering with its amphiphilic alpha-helical fold. Finally, to illustrate the numerous uses of l-4F-Phg in (19)F NMR spectroscopy, we characterised the interaction of labelled PGLa with uncharged and negatively charged membranes. Observing the signal of the free peptide in an aqueous suspension of unilamellar vesicles, we found a linear saturation behaviour that was dominated by electrostatic attraction of the cationic PGLa. Once the peptide is bound to the membrane, however, solid-state (19)F NMR spectroscopy of macroscopically oriented samples revealed that the charge density has virtually no further influence on the structure, alignment or mobility of the peptide.

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Clonal dissemination of two MRSA strains in Germany

Cited by 28 publications

References 20 publications

Unusual Spread of a Penicillin-Susceptible Methicillin-Resistant Staphylococcus aureus Clone in a Geographic Area of Low Incidence

Unusual Spread of a Penicillin-Susceptible Methicillin-Resistant Staphylococcus aureus Clone in a Geographic Area of Low Incidence

Emergence and spread of antibiotic-resistant Gram-positive bacterial pathogens

4‐Fluorophenylglycine as a Label for ¹⁹F NMR Structure Analysis of Membrane‐Associated Peptides

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Clonal dissemination of two MRSA strains in Germany

Cited by 28 publications

References 20 publications

Unusual Spread of a Penicillin-Susceptible Methicillin-Resistant Staphylococcus aureus Clone in a Geographic Area of Low Incidence

Unusual Spread of a Penicillin-Susceptible Methicillin-Resistant Staphylococcus aureus Clone in a Geographic Area of Low Incidence

Emergence and spread of antibiotic-resistant Gram-positive bacterial pathogens

4‐Fluorophenylglycine as a Label for 19F NMR Structure Analysis of Membrane‐Associated Peptides

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4‐Fluorophenylglycine as a Label for ¹⁹F NMR Structure Analysis of Membrane‐Associated Peptides