Clonal dissemination of epidemic methicillin-resistant Staphylococcus aureus in Belgium and neighboring countries

Deplano, Ariane; Witte, Wolfgang; Leeuwen, W. J. Van; Brun, Y.; Struelens, Marc

doi:10.1046/j.1469-0691.2000.00064.x

Cited by 83 publications

(77 citation statements)

References 18 publications

(47 reference statements)

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“…For all isolates, SmaI restriction fragments of genomic DNA were separated by pulsed-field gel electrophoresis (PFGE) as previously described (22). Bands in a size range between 36 and 700 kb were analyzed using the Bionumerics software version 6.5 by the Dice similarity coefficient, with an optimization set to 0.8% and a position tolerance set to 1%.…”

Section: Iiia Ant(4=)-ia Aac(6=)-ie Plus Aph(2љ) Erm(a) Erm(b) Ementioning

confidence: 99%

Comparative Epidemiology of Staphylococcus epidermidis Isolates from Patients with Catheter-Related Bacteremia and from Healthy Volunteers

et al. 2013

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Staphylococcus epidermidis is a major cause of catheter-related bloodstream infections (CRBSIs). Recent studies suggested the existence of well-adapted, highly resistant, hospital-associated S. epidermidis clones. The molecular epidemiology of S. epidermidis in Belgian hospitals and the Belgian community has not been explored yet. We compared a set of 33 S. epidermidis isolates causing CRBSI in hospitalized patients with a set of 33 commensal S. epidermidis isolates. The factors analyzed included resistance to antibiotics and genetic diversity as determined by pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), and SCCmec typing. Additionally, the presence of virulence-associated mobile genetic elements, the ica operon and the arginine catabolic mobile element (ACME), was assessed and compared against clinical data. CRBSI S. epidermidis isolates were significantly resistant to more antibiotics than commensal S. epidermidis isolates. The two populations studied were very diverse and genetically distinct as only 23% of the 37 PFGE types observed were harbored by both CRBSI and commensal isolates. ACME was found in 76% of S. epidermidis strains, regardless of their origin, while the ica operon was significantly more prevalent in CRBSI isolates than in commensal isolates (P < 0.05). Nine patients presented a clinically severe CRBSI, eight cases of which were due to an ica-positive multiresistant isolate belonging to sequence type 2 (ST2) or ST54. S. epidermidis isolates causing CRBSI were more resistant and more often ica positive than commensal S. epidermidis isolates, which were genetically heterogeneous and susceptible to the majority of antibiotics tested. Clinically severe CRBSIs were due to isolates belonging to two closely related MLST types, ST2 and ST54.

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Section: Iiia Ant(4=)-ia Aac(6=)-ie Plus Aph(2љ) Erm(a) Erm(b) Ementioning

confidence: 99%

Comparative Epidemiology of Staphylococcus epidermidis Isolates from Patients with Catheter-Related Bacteremia and from Healthy Volunteers

et al. 2013

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“…Staphylococcus aureus continues to be a major cause of health care-associated infections (1,18,19,45,53). Recently, strains of methicillin (oxacillin)-resistant S. aureus (MRSA) have been recovered from infections in community settings (8,11,27,44,49,64) and among the urban poor in San Francisco, California (12).…”

mentioning

confidence: 99%

Characterization of a Strain of Community-AssociatedMethicillin-Resistant Staphylococcus aureus WidelyDisseminated in the UnitedStates

et al. 2006

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A highly stable strain of Staphylococcus aureus with a pulsed-field gel electrophoresis type of USA300 and multilocus sequence type 8 has been isolated from patients residing in diverse geographic regions of the United States. This strain, designated USA300-0114, is a major cause of skin and soft tissue infections among persons in community settings, including day care centers and correctional facilities, and among sports teams, Native Americans, men who have sex with men, and military recruits. The organism is typically resistant to penicillin, oxacillin, and erythromycin (the latter mediated by msrA) and carries SCCmec type IVa. This strain is variably resistant to tetracycline [mediated by tet(K)]; several recent isolates have decreased susceptibility to fluoroquinolones. S. aureus USA300-0114 harbors the genes encoding the Panton-Valentine leucocidin toxin. DNA sequence analysis of the direct repeat units within the mec determinant of 30 USA300-0114 isolates revealed differences in only a single isolate. Plasmid analysis identified a common 30-kb plasmid that hybridized with blaZ and msrA probes and a 3.1-kb cryptic plasmid. A 4.3-kb plasmid encoding tet(K) and a 2.6-kb plasmid encoding ermC were observed in a few isolates. DNA microarray analysis was used to determine the genetic loci for a series of virulence factors and genes associated with antimicrobial resistance. Comparative genomics between USA300-0114 and three other S. aureus lineages (USA100, USA400, and USA500) defined a set of USA300-0114-specific genes, which may facilitate the strain's pathogenesis within diverse environments.

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“…This MRSA clone was first described in Spain, 1989(Dominguez et al 1994) and later on, in Portugal (Ferrari et al 2002), Italy and United Kingdom (Mato et al 1998), Germany (Witte et al 1994), Belgium, Switzerland, France (Deplano et al 2000), Poland (Leski et al 1998), Check Republic (Melter et al 2003), and United States (Roberts et al 1998a).…”

Section: Evolution Of Typing Techniques and The Characterization Of Mmentioning

confidence: 97%

Application of molecular techniques in the study of Staphylococcus aureus clonal evolution - A Review

Vivoni

Moreira

2005

Mem. Inst. Oswaldo Cruz

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Clonal dissemination of epidemic methicillin-resistant Staphylococcus aureus in Belgium and neighboring countries

Cited by 83 publications

References 18 publications

Comparative Epidemiology of Staphylococcus epidermidis Isolates from Patients with Catheter-Related Bacteremia and from Healthy Volunteers

Comparative Epidemiology of Staphylococcus epidermidis Isolates from Patients with Catheter-Related Bacteremia and from Healthy Volunteers

Characterization of a Strain of Community-AssociatedMethicillin-Resistant Staphylococcus aureus WidelyDisseminated in the UnitedStates

Application of molecular techniques in the study of Staphylococcus aureus clonal evolution - A Review

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