Clonal Composition of<i>Escherichia coli</i>Causing Community-Acquired Urinary Tract Infections in the State of Rio de Janeiro, Brazil

Dias, Rubens C. S.; Marangoni, D; Smith, Sherry P.; Alves, Elizabeth M.; Pellegrino, Flávia Lúcia Piffano Costa; Riley, Lee W.; Moreira, Beatriz Meurer

doi:10.1089/mdr.2009.0067

Cited by 41 publications

(46 citation statements)

References 33 publications

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“…These sequence types have also previously been isolated from food products and include the ST10 complex, ST69, ST117, ST131, and ST405 (30). ST69, previously labeled CgA, was first reported from UTI patients in Berkeley, California, USA, and has also been reported from UTI patients in Rio de Janeiro, Brazil (31,32). ST62 has previously been associated with K1 E. coli, a serotype that frequently causes neonatal meningitis (20).…”

Section: Discussionmentioning

confidence: 99%

Distribution of Strain Type and Antimicrobial Susceptibility of Escherichia coli Isolates Causing Meningitis in a Large Urban Setting in Brazil

et al. 2014

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The clinical management of meningitis caused by Escherichia coli is greatly complicated when the organism becomes resistant to broad-spectrum antibiotics. We sought to characterize the antimicrobial susceptibilities, sequence types (ST), and presence of known drug resistance genes of E. coli isolates that caused meningitis between 1996 and 2011 in Salvador, Brazil. We then compared these findings to those for E. coli isolates from community-acquired urinary tract infections (UTI) that occurred during the same time period and in the same city. We found that 19% of E. coli isolates from cases of meningitis and less than 1% of isolates from UTI were resistant to third-generation cephalosporins. The sequence types of E. coli isolates from cases of meningitis included ST131, ST69, ST405, and ST62, which were also found among isolates from UTI. Additionally, among the E. coli isolates that were resistant to third-generation cephalosporins, we found genes that encode the extended-spectrum beta-lactamases CTX-M-2, CTX-M-14, and CTX-M-15. These observations demonstrate that compared to E. coli strains isolated from cases of community-acquired UTI, those isolated from cases of meningitis are more resistant to third-generation cephalosporins, even though the same sequence types are shared between the two forms of extraintestinal infections.

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Section: Discussionmentioning

confidence: 99%

Distribution of Strain Type and Antimicrobial Susceptibility of Escherichia coli Isolates Causing Meningitis in a Large Urban Setting in Brazil

et al. 2014

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“…On the other hand, MLST itself may be less discriminatory than PFGE. In the study of UPEC, isolates included in one ST are usually also clustered by PFGE with at least 68% similarity (4,15,20).…”

Section: Discussionmentioning

confidence: 99%

“…One hundred twenty-seven and 12 isolates were recovered from urine samples of women and men, respectively, with UTI in the community of Rio de Janeiro (4,5); 42 hospital isolates were recovered from different clinical specimens (including blood, lower respiratory tract, surgical site, normally sterile secretions and tissues, catheter tip, and other sources) of patients (22 men and 20 women) at a public university-affiliated hospital (3); and 120 and 41 isolates were recovered from urine samples of hospitalized female and male patients, respectively (unpublished data). Only one isolate per patient was included.…”

Section: Bacterial Isolates Between 2001 and 2006mentioning

confidence: 99%

“…Additional strain typing by E. coli reference collection (ECOR) phylogenetic grouping, pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), serotyping, and virulence profiling revealed that the CgA strains belonged to an identical or a very closely related lineage of E. coli (9,14,20). In 2009, in Rio de Janeiro, Brazil, CgA was also found to comprise the largest clonal group observed among women with community-acquired UTI (4).…”

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Use of fimH Single-Nucleotide Polymorphisms for Strain Typing of Clinical Isolates of Escherichia coli for Epidemiologic Investigation

2010

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Strain typing methods that compare electrophoresis banding patterns are commonly used but are difficult to standardize and poorly portable. Multilocus sequence typing (MLST) is a sequence-based alternative, but it is not practical for large-scale epidemiological studies. In the present study, the usefulness of fimH singlenucleotide polymorphisms (SNPs) for Escherichia coli typing was explored. fimH SNPs were determined for 345 E. coli clinical isolates (including 3 reference strains) and compared to PCR-based ECOR (E. coli reference collection) phylogrouping. The fimH gene could be amplified for 316 (92%) of the 345 isolates. fimH SNP analysis found 46 distinct terminal groups in the nucleotide sequence-based phylogenetic tree (fimH types). A subset of the E. coli isolates (162 clinical isolates and the 3 reference strains) were compared by fimH type, PCR phylogroup, and MLST. These isolates fell into 27 fimH types and 18 MLST clonal complexes (CCs) that contained 2 to 28 isolates per complex. The combination of PCR phylogroup and fimH type corresponded to a single CC for 113 (68%) isolates and 2 or 3 CCs for the other 52 (32%) isolates. We propose that the combination of PCR phylogrouping and fimH SNP analysis may be a useful method to type a large collection of clinical E. coli isolates for epidemiologic studies.Molecular strain typing methods have enhanced our understanding of the epidemiology of many infectious diseases by contributing to the characterization of new modes of transmission, vehicles, and risk factors for infections. Recently, a new understanding about the epidemiology of community-acquired urinary tract infections (UTI) emerged from the systematic strain typing analysis of uropathogenic Escherichia coli (UPEC). Community-acquired UTI typically occurs as an endemic infection. In 2001, Manges and colleagues identified clusters of UTI on 3 different college campuses in the United States caused by an E. coli clonal group that was designated CgA based on a characteristic enterobacterial repetitive intergenic consensus (ERIC)-PCR electrophoresis banding pattern (14). Additional strain typing by E. coli reference collection (ECOR) phylogenetic grouping, pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), serotyping, and virulence profiling revealed that the CgA strains belonged to an identical or a very closely related lineage of E. coli (9,14,20). In 2009, in Rio de Janeiro, Brazil, CgA was also found to comprise the largest clonal group observed among women with community-acquired UTI (4).Although reference laboratory surveillance systems using PFGE, such as PulseNet, have been highly successful, the analysis of multiple electrophoresis banding patterns is subject to interlaboratory variability and is not amenable to simple standardization for portability and the creation of public databases. MLST was developed as an attractive sequence-based genotyping technique because it provides reproducibility, comparability, and transferability between laboratories. Although MLST seems...

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“…The CgA isolates are closely related to the clonal O15:K52:H1 group. CgA isolates are characterized by belonging to phylogroup D and MLST clonal complex 69 (CC69) and often possess resistance to trimethoprim-sulfonamides (3,9). In Denmark, the frequency of resistance to sulfonamides-the drugs of choice for treatment of uncomplicated UTI-has remained constant (Ϸ40%) during the past years despite a decline in their use in humans.…”

Section: Urinary Tract Infection (Uti) Is One Of the Most Common Bactmentioning

confidence: 99%

Detection of Clonal Group A Escherichia coli Isolates from Broiler Chickens, Broiler Chicken Meat, Community-Dwelling Humans, and Urinary Tract Infection (UTI) Patients and Their Virulence in a Mouse UTI Model

Jakobsen

Hammerum

Frimodt‐Møller

2010

Appl Environ Microbiol

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Escherichia coli clonal group A isolates cause infections in people. We investigated 158 phylogroup D E. coli isolates from animals, meat, and humans. Twenty-five of these isolates were of clonal group A, and 15 isolates were shown to cause infection in a mouse urinary tract infection (UTI) model. We conclude that clonal group A isolates are found in both broiler chickens and broiler chicken meat and may cause UTI in humans.

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Clonal Composition ofEscherichia coliCausing Community-Acquired Urinary Tract Infections in the State of Rio de Janeiro, Brazil

Cited by 41 publications

References 33 publications

Distribution of Strain Type and Antimicrobial Susceptibility of Escherichia coli Isolates Causing Meningitis in a Large Urban Setting in Brazil

Distribution of Strain Type and Antimicrobial Susceptibility of Escherichia coli Isolates Causing Meningitis in a Large Urban Setting in Brazil

Use of fimH Single-Nucleotide Polymorphisms for Strain Typing of Clinical Isolates of Escherichia coli for Epidemiologic Investigation

Detection of Clonal Group A Escherichia coli Isolates from Broiler Chickens, Broiler Chicken Meat, Community-Dwelling Humans, and Urinary Tract Infection (UTI) Patients and Their Virulence in a Mouse UTI Model

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