Clonal analysis and virulent traits of pathogenic extraintestinal Escherichia coliisolates from swine in China

Ding, Yi; Tang, Xibiao; Lü, Ping; Wu, Bin; Zhao, Xu; Liu, Wugang; Zhang, Ruixuan; Bei, Weicheng; Chen, Huanchun; Tan, Chen

doi:10.1186/1746-6148-8-140

Cited by 35 publications

(35 citation statements)

References 28 publications

(34 reference statements)

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“…Two previous studies in China have found that CC10 was frequently identified among ESBLproducing or non-ESBL-producing E. coli isolates from food animals. 6,11 These studies, along with this study, indicate a correlation between ESBL-producing E. coli isolates in food animals and humans. Only four ESBL-producers were identified as the B2-ST131 clone harboring bla CTX-M-14 , which is different from many studies in which the CTX-M-15 carrier predominated among B2-ST131.…”

Section: Discussionsupporting

confidence: 65%

High Prevalence of CTX-M Beta-Lactamases in Enterobacteriaceae from Healthy Individuals in Guangzhou, China

Yong

Zhang

et al. 2015

Microbial Drug Resistance

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This study aimed to determine the prevalence of extended-spectrum beta-lactamases (ESBLs) in Enterobacteriaceae and to characterize the genetic composition of ESBL determinants among Enterobacteriaceae isolates from healthy people in Guangzhou, China. A total of 200 rectal swab samples were collected from healthy asymptomatic individuals and tested for ESBL production using ChromID ESBL agar. Phenotypic ESBL producers were screened for blaCTX-M, blaTEM, and blaSHV genes using PCR and DNA sequencing. The prevalence of ESBL-producing Enterobacteriaceae among rectal swab samples was 69.5%. All ESBL-producing isolates harbored blaCTX-M genes (n=138) except for one isolate that harbored blaSHV-2a. Eleven CTX-M ESBL genes were detected. The most predominant CTX-M-type genes were blaCTX-M-14 (n=82), followed by blaCTX-M-55 (n=19), blaCTX-M-65 (n=10), and blaCTX-M-27 (n=9). Isolates carrying blaCTX-M-38,-3,-15,-14b,-98,-121 and -123 were also identified. Molecular homology analysis of the selected isolates was performed by phylogenetic grouping and multilocus sequence typing and indicated that the predominant clone belonged to A-CC10. This study showed a high rate of CTX-M-type ESBL genes among Enterobacteriaceae isolates from healthy individuals in China.

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Section: Discussionsupporting

confidence: 65%

High Prevalence of CTX-M Beta-Lactamases in Enterobacteriaceae from Healthy Individuals in Guangzhou, China

Yong

Zhang

et al. 2015

Microbial Drug Resistance

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“…The ST10, ST58, and ST155 clones have been detected in animals, water, and human isolates in the Netherlands, Canada, Chile, and China (28,30,35,(38)(39)(40)(41)(42)(43)(44)(45). We detected numerous ST10 or ST58 clonal complexes in high-density chicken-farming areas.…”

Section: Discussionmentioning

confidence: 72%

“…As shown in Table 5, various ESBL-producing E. coli clones have been isolated from animals, although ST131 clones have not been detected in certain studies (28,30,(35)(36)(37)(38)(39)(40)(42)(43)(44)(45). The ST10, ST58, and ST155 clones have been detected in animals, water, and human isolates in the Netherlands, Canada, Chile, and China (28,30,35,(38)(39)(40)(41)(42)(43)(44)(45).…”

Section: Discussionmentioning

confidence: 99%

Characteristics of CTX-M Extended-Spectrum β-Lactamase-Producing Escherichia coli Strains Isolated from Multiple Rivers in Southern Taiwan

Chen

Hung

Huang

et al. 2016

Appl Environ Microbiol

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h Extended-spectrum ␤-lactamase (ESBL)-producing Escherichia coli sequence type ST131 has emerged as the leading cause of community-acquired urinary tract infections and bacteremia worldwide. Whether environmental water is a potential reservoir of these strains remains unclear. River water samples were collected from 40 stations in southern Taiwan from February to August 2014. PCR assay and multilocus sequence typing (MLST) analysis were conducted to determine the CTX-M group and sequence type, respectively. In addition, we identified the seasonal frequency of ESBL-producing E. coli strains and their geographical relationship with runoffs from livestock and poultry farms between February and August 2014. ESBL-producing E. coli accounted for 30% of the 621 E. coli strains isolated from river water in southern Taiwan. ESBL-producing E. coli ST131 was not detected among the isolates. The most commonly detected strain was E. coli CTX-M group 9. Among the 92 isolates selected for MLST analysis, the most common ESBL-producing clonal complexes were ST10 and ST58. The proportion of ESBL-producing E. coli was significantly higher in areas with a lower river pollution index (P ‫؍‬ 0.025) and regions with a large number of chickens being raised (P ‫؍‬ 0.013). ESBL-producing E. coli strains were commonly isolated from river waters in southern Taiwan. The most commonly isolated ESBL-producing clonal complexes were ST10 and ST58, which were geographically related to chicken farms. ESBL-producing E. coli ST131, the major clone causing community-acquired infections in Taiwan and worldwide, was not detected in river waters.

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“…ExPEC are also among the top causes for neonatal bacterial meningitis in newborns (Stoll et al, 2002). In addition, ExPEC cause septicemia and sudden death in swine and avian species leading tosignificant economic losses for these industries (Dho-Moulin and Fairbrother, 1999;Ding et al, 2012). Although there are different subtypes of ExPEC including uropathogenic E. coli (UPEC), neonatal meningitis-associated E. coli (NMEC) and septicemia-associated E.coli (SEPEC), the common feature of many ExPEC is their ability to cause bloodstream infection leading to septicemia (Kaper, 2005).…”

Section: Introductionmentioning

confidence: 99%

Extraintestinal pathogenic Escherichia coli increase extracytoplasmic polysaccharide biosynthesis for serum resistance in response to bloodstream signals

Jiang

et al. 2018

Molecular Microbiology

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Extraintestinal pathogenic Escherichia coli (ExPEC) is one of the leading causes of bloodstream infections. Characteristically, these organisms exhibit strong resistance to the bactericidal action of host serum. Although numerous serum resistance factors in ExPEC have been identified, their regulatory mechanisms during in vivo infection remain largely unknown. Here, RNA sequencing analyses together with quantitative reverse-transcription PCR revealed that ExPEC genes involved in the biosynthesis of extracytoplasmic polysaccharides (ECPs) including K-capsule, lipopolysaccharide (LPS), colanic acid, peptidoglycan and Yjb exopolysaccharides were significantly upregulated in response to serum under low oxygen conditions and during bloodstream infection. The oxygen sensor FNR directly activated the expression of K-capsule and colanic acid and also indirectly modulated the expression of colanic acid, Yjb exopolysaccharides and peptidoglycan via the known Rcs regulatory system. The global regulator Fur directly or indirectly repressed the expression ofECP biosynthesis genes in iron replete media, whereas the low iron conditions in the bloodstream could relieve Fur repression. Using in vitro and animal models, FNR, Fur and the Rcs system were confirmed as contributing to ExPEC ECP production, serum resistance and virulence. Altogether, these findings indicated that the global regulators FNR andFur and the signaling transduction system Rcs coordinately regulated the expression of ECP biosynthesis genes leading to increased ExPEC serum resistance in response to low oxygen and low iron levels in the bloodstream.

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Clonal analysis and virulent traits of pathogenic extraintestinal Escherichia coliisolates from swine in China

Cited by 35 publications

References 28 publications

High Prevalence of CTX-M Beta-Lactamases in Enterobacteriaceae from Healthy Individuals in Guangzhou, China

High Prevalence of CTX-M Beta-Lactamases in Enterobacteriaceae from Healthy Individuals in Guangzhou, China

Characteristics of CTX-M Extended-Spectrum β-Lactamase-Producing Escherichia coli Strains Isolated from Multiple Rivers in Southern Taiwan

Extraintestinal pathogenic Escherichia coli increase extracytoplasmic polysaccharide biosynthesis for serum resistance in response to bloodstream signals

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