Clomiphene citrate modulates the expression of endometrial carbohydrates (especially N-acetyl-d-glucosamine and sialic acid) in pseudopregnant rats

Khoza, Tshepo David; Hosie, Margot J.

doi:10.1016/j.theriogenology.2008.04.008

Cited by 3 publications

(1 citation statement)

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“…In addition, injection of LIF could modulate glycoconjugates on the apical surface of the murine uterine epithelium including terminal sialic acid [36]. However, the expression and roles of sialic acid epitopes in the embryo implantation site are still controversial [34,35,37]. In this study, we firstly found that the expression of a unique α2,6 sialic acid epitopes, sialyl-Tn antigen on the surface of endometrial cells might play a crucial role in receptivity toward trophoblast during the implantation window period.…”

Section: Discussionmentioning

confidence: 83%

Expression of ST6GalNAc1 and sialyl-Tn antigen enhances endometrial receptivity

Choi¹,

Chung²,

Kim³

et al. 2020

Preprint

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BackgroundTo characterize molecular mechanism underlying the regulation of sialylated glycan expression and its roles for endometrial receptivity and embryo implantation. Here, we characterized the role of a truncated form of sialylated O-glycan, sialyl-Tn, for endometrial receptivity. MethodsThe transcriptomes of human endometrium at mid-secretory phase were analyzed by Bioinformatics.Changes in gene expression, protein, and signal pathway were measured using RT-PCR and Western blot. The cell adhesion assay was visualized using a fluorescent microscope. In peri-implantation phase of mice, the expression of leukemia inhibitory factor (LIF) and sialyl-Tn were confirmed using immunohistochemistry and immnofluorescence analysis. The effect of sialy-Tn expression on embryo implantation was estimated by in vitro fertilization and embryo transfer using mice. ResultsIn in silico analysis, expression of O-glycosylation genes, especially ST6GalNAc1, was significantly increased in the human uterus of mid-secretory phase. Overexpression of the ST6GalNAc1 gene in non-receptive human endometrial AN3CA cells enhances the attachment of trophoblastic JAr cells. In an animal study, the results clearly indicated that sialyl-Tn was expressed on the surface of the midsecretory uterus. In addition, blockade of the receptor using free sialyl-Tn epitope diminished the implantation rates of intrauterine transferred murine embryos. ConclusionFrom these results, here we suggest that sialyl-Tn expression might be a novel factor regulating the endometrial receptivity for successful embryo implantation. BackgroundEmbryo implantation, a key process for a successful pregnancy, is one of the remaining unmet needs to improve the success rate of assisted reproduction [1]. The endometrium is receptive to embryo only during the implantation window, a short period of time about 6-10 days after ovulation [1,2]. A large number of molecular mediators including adhesive proteins, hormones, cytokines, and growth Authors' contributions HJC and TWC designed the experiments and performed the major molecular experiment. JK and JHJ performed in vitro fertilization and embryo transfer. BSK and MJP assisted the molecular experiments. KJK perfoermed the immunohistochemistry and immunoflurescent staining. JKJ and CHK analyzed the

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Section: Discussionmentioning

confidence: 83%

Expression of ST6GalNAc1 and sialyl-Tn antigen enhances endometrial receptivity

Choi¹,

Chung²,

Kim³

et al. 2020

Preprint

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Early and natural embryonic death in Lagostomus maximus: Association with the uterine glands, vasculature, and musculature

Acuña

Barbeito

Portiansky

et al. 2020

Journal of Morphology

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The uterus is an organ with great plasticity due to the morphological and physiological changes it experiences during the estrous cycle and pregnancy. In mammals, pregnancy requires diverse sex hormones, growth factors and cytokines, among others, for promoting uterine remodeling to favor implantation, placentation, and embryo/fetus survival and growth. The hystricognathi rodent Lagostomus maximus (plains viscacha) has a high rate of embryonic resorption. The cranial and middle implants are reabsorbed 25–35 days after intercourse while the caudal embryos continue with their development until two precocial offspring are born. So far, no uterine studies of non‐pregnant L. maximus females were performed to determine the possible existence of variations in the organ that could be related to the differential survival of the implants. We used ultrasonography, as well as morphological, morphometric, histochemical, lectinhistochemical, and immunohistochemical methods to study differences in the uterine glands (area), vasculature (area), and musculature (thickness) along the uterine horns in non‐pregnant females. Along the uterus, all these structures were in more advanced developmental condition in the caudal region as compared to more anterior positions. These regional variations could be decisive in explaining the reason why only caudal implantations come to term. In contrast, no differences in the in the luminal and glandular epithelial cells, nor in the degree of cell proliferation and apoptosis, and hormonal receptor staining were found. These parameters could be related to implantation along the uterine horns, but not to the differential survival of the implants.

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Sialyltransferase, Sialylation, and Sulfoylation

Kim

2020

Ganglioside Biochemistry

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Clomiphene citrate modulates the expression of endometrial carbohydrates (especially N-acetyl-d-glucosamine and sialic acid) in pseudopregnant rats

Cited by 3 publications

References 51 publications

Expression of ST6GalNAc1 and sialyl-Tn antigen enhances endometrial receptivity

Expression of ST6GalNAc1 and sialyl-Tn antigen enhances endometrial receptivity

Early and natural embryonic death in Lagostomus maximus: Association with the uterine glands, vasculature, and musculature

Sialyltransferase, Sialylation, and Sulfoylation

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